ID   VASO3_TABYA             Reviewed;          76 AA.
AC   C8YJB4;
DT   03-AUG-2022, integrated into UniProtKB/Swiss-Prot.
DT   03-NOV-2009, sequence version 1.
DT   03-AUG-2022, entry version 20.
DE   RecName: Full=Vasotab-TY3 {ECO:0000303|PubMed:19531497};
DE   Flags: Precursor;
OS   Tabanus yao (Horsefly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Tabanomorpha; Tabanoidea;
OC   Tabanidae; Tabanus.
OX   NCBI_TaxID=485572;
RN   [1] {ECO:0000312|EMBL:ACS72309.1}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 22-50, SUBCELLULAR
RP   LOCATION, TISSUE SPECIFICITY, AND MASS SPECTROMETRY.
RC   TISSUE=Salivary gland;
RX   PubMed=19531497; DOI=10.1074/mcp.m900186-mcp200;
RA   Ma D., Wang Y., Yang H., Wu J., An S., Gao L., Xu X., Lai R.;
RT   "Anti-thrombosis repertoire of blood-feeding horsefly salivary glands.";
RL   Mol. Cell. Proteomics 8:2071-2079(2009).
CC   -!- FUNCTION: Vasodilator protein that inhibits vasoconstriction of
CC       isolated rat femoral artery induced by phenylephrine. Since platelet
CC       aggregation and vasoconstriction are key hemostatic responses,
CC       particularly in small wounds, this protein likely participates in the
CC       antihemostatic responses during blood feeding. Blocks L-type calcium
CC       channels (Cav1/CACNA1) in left ventricular myocytes isolated from rat
CC       hearts. {ECO:0000250|UniProtKB:P84843}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:19531497}.
CC   -!- TISSUE SPECIFICITY: Expressed by the salivary gland.
CC       {ECO:0000269|PubMed:19531497}.
CC   -!- MASS SPECTROMETRY: Mass=5975.1; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:19531497};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FJ469621; ACS72309.1; -; mRNA.
DR   InterPro; IPR002350; Kazal_dom.
DR   InterPro; IPR036058; Kazal_dom_sf.
DR   Pfam; PF00050; Kazal_1; 1.
DR   SMART; SM00280; KAZAL; 1.
DR   SUPFAM; SSF100895; SSF100895; 1.
DR   PROSITE; PS51465; KAZAL_2; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Secreted; Signal; Toxin; Vasoactive;
KW   Vasodilator; Voltage-gated calcium channel impairing toxin.
FT   SIGNAL          1..21
FT                   /evidence="ECO:0000305|PubMed:19531497"
FT   CHAIN           22..76
FT                   /note="Vasotab-TY3"
FT                   /evidence="ECO:0000305"
FT                   /id="PRO_5002993623"
FT   DOMAIN          22..76
FT                   /note="Kazal-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        23..60
FT                   /evidence="ECO:0000250|UniProtKB:P84843,
FT                   ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        27..53
FT                   /evidence="ECO:0000250|UniProtKB:P84843,
FT                   ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        35..75
FT                   /evidence="ECO:0000250|UniProtKB:P84843,
FT                   ECO:0000255|PROSITE-ProRule:PRU00798"
SQ   SEQUENCE   76 AA;  8262 MW;  F16163B6D50888E9 CRC64;
     MKFALFSVLV LMLIATFVAA DDCPRICTSD YTPVCGTPSG GRRSANRTFA NQCGLDSHNC
     LNKGDTYDKL HDGECK