ID   VASOT_HYBBI             Reviewed;          76 AA.
AC   P84843;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   02-MAY-2006, sequence version 1.
DT   03-AUG-2022, entry version 41.
DE   RecName: Full=Vasotab {ECO:0000303|PubMed:16391356};
DE   Flags: Precursor;
OS   Hybomitra bimaculata (Horse fly).
OC   Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC   Neoptera; Endopterygota; Diptera; Brachycera; Tabanomorpha; Tabanoidea;
OC   Tabanidae; Hybomitra.
OX   NCBI_TaxID=226809;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 21-67, FUNCTION,
RP   SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MASS SPECTROMETRY.
RC   TISSUE=Salivary gland;
RX   PubMed=16391356; DOI=10.1242/jeb.02003;
RA   Takac P., Nunn M.A., Meszaros J., Pechanova O., Vrbjar N., Vlasakova P.,
RA   Kozanek M., Kazimirova M., Hart G., Nuttall P.A., Labuda M.;
RT   "Vasotab, a vasoactive peptide from horse fly Hybomitra bimaculata
RT   (Diptera, Tabanidae) salivary glands.";
RL   J. Exp. Biol. 209:343-352(2006).
CC   -!- FUNCTION: Vasodilator protein that inhibits vasoconstriction of
CC       isolated rat femoral artery induced by phenylephrine. Since platelet
CC       aggregation and vasoconstriction are key hemostatic responses,
CC       particularly in small wounds, this protein likely participates in the
CC       antihemostatic responses during blood feeding. Blocks L-type calcium
CC       channels (Cav1/CACNA1) in left ventricular myocytes isolated from rat
CC       hearts. {ECO:0000269|PubMed:16391356}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16391356}.
CC   -!- TISSUE SPECIFICITY: Expressed by the salivary gland.
CC       {ECO:0000269|PubMed:16391356}.
CC   -!- MASS SPECTROMETRY: Mass=6141; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:16391356};
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DR   AlphaFoldDB; P84843; -.
DR   SMR; P84843; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0019855; F:calcium channel inhibitor activity; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   GO; GO:0005245; F:voltage-gated calcium channel activity; IDA:UniProtKB.
DR   GO; GO:0045986; P:negative regulation of smooth muscle contraction; IDA:UniProtKB.
DR   GO; GO:0042311; P:vasodilation; IEA:UniProtKB-KW.
DR   InterPro; IPR002350; Kazal_dom.
DR   InterPro; IPR036058; Kazal_dom_sf.
DR   Pfam; PF07648; Kazal_2; 1.
DR   SMART; SM00280; KAZAL; 1.
DR   SUPFAM; SSF100895; SSF100895; 1.
DR   PROSITE; PS51465; KAZAL_2; 1.
PE   1: Evidence at protein level;
KW   Calcium channel impairing toxin; Direct protein sequencing; Disulfide bond;
KW   Ion channel impairing toxin; Secreted; Signal; Toxin; Vasoactive;
KW   Vasodilator; Voltage-gated calcium channel impairing toxin.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000269|PubMed:16391356"
FT   CHAIN           21..76
FT                   /note="Vasotab"
FT                   /evidence="ECO:0000269|PubMed:16391356"
FT                   /id="PRO_0000233909"
FT   DOMAIN          21..76
FT                   /note="Kazal-like"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798"
FT   DISULFID        23..60
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798,
FT                   ECO:0000269|PubMed:16391356"
FT   DISULFID        27..53
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798,
FT                   ECO:0000269|PubMed:16391356"
FT   DISULFID        35..75
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00798,
FT                   ECO:0000269|PubMed:16391356"
SQ   SEQUENCE   76 AA;  8282 MW;  14C43EC91C278D9C CRC64;
     MKFALFSVLV VLLIATFVAA DECPRICTAD YRPVCGTPSG GRRSANRTFG NQCSLNAHNC
     LNKGDTYDKL HDGECK