ID   VASX_VIBCH              Reviewed;        1085 AA.
AC   Q9KNE5;
DT   26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 79.
DE   RecName: Full=Toxin VasX {ECO:0000303|PubMed:24348240};
GN   Name=vasX {ECO:0000303|PubMed:24348240}; OrderedLocusNames=VC_A0020;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=24348240; DOI=10.1371/journal.ppat.1003752;
RA   Miyata S.T., Unterweger D., Rudko S.P., Pukatzki S.;
RT   "Dual expression profile of type VI secretion system immunity genes
RT   protects pandemic Vibrio cholerae.";
RL   PLoS Pathog. 9:E1003752-E1003752(2013).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=23362380; DOI=10.1073/pnas.1222783110;
RA   Dong T.G., Ho B.T., Yoder-Himes D.R., Mekalanos J.J.;
RT   "Identification of T6SS-dependent effector and immunity proteins by Tn-seq
RT   in Vibrio cholerae.";
RL   Proc. Natl. Acad. Sci. U.S.A. 110:2623-2628(2013).
CC   -!- FUNCTION: Toxin secreted by the type VI (T6SS) secretion system that
CC       acts on prokaryotic target cells. Acts in conjunction with VasW, an
CC       accessory protein to VasX, to compromise the inner membrane of
CC       prokaryotic target cells. {ECO:0000269|PubMed:23362380,
CC       ECO:0000269|PubMed:24348240}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:24348240}. Host
CC       membrane {ECO:0000255}; Multi-pass membrane protein {ECO:0000255}.
CC       Note=Delivered to the target cell by the type VI (T6SS) secretion
CC       system. {ECO:0000269|PubMed:23362380}.
CC   -!- DISRUPTION PHENOTYPE: Deletion of vasX gene together with deletion of
CC       vgrG3 fail to kill Escherichia coli. {ECO:0000269|PubMed:23362380}.
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DR   EMBL; AE003853; AAF95934.1; -; Genomic_DNA.
DR   PIR; H82511; H82511.
DR   RefSeq; NP_232421.1; NC_002506.1.
DR   RefSeq; WP_000070352.1; NZ_LT906615.1.
DR   AlphaFoldDB; Q9KNE5; -.
DR   STRING; 243277.VC_A0020; -.
DR   TCDB; 1.C.131.1.1; the vasx toxin (vasx) family.
DR   PRIDE; Q9KNE5; -.
DR   DNASU; 2612513; -.
DR   EnsemblBacteria; AAF95934; AAF95934; VC_A0020.
DR   GeneID; 57741495; -.
DR   KEGG; vch:VC_A0020; -.
DR   PATRIC; fig|243277.26.peg.2667; -.
DR   eggNOG; ENOG502ZW1U; Bacteria.
DR   HOGENOM; CLU_009101_0_0_6; -.
DR   OMA; NITIYLD; -.
DR   BioCyc; VCHO:VCA0020-MON; -.
DR   Proteomes; UP000000584; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Host membrane; Membrane; Reference proteome; Secreted; Transmembrane;
KW   Transmembrane helix; Virulence.
FT   CHAIN           1..1085
FT                   /note="Toxin VasX"
FT                   /id="PRO_0000449209"
FT   TRANSMEM        753..773
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        813..833
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        860..880
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        884..904
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          1..20
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1085 AA;  121043 MW;  D176D85571729945 CRC64;
     MSNPNQAAKT GQTNDAQNPA SACPFKQPLI GIIPVRYAFD VYDDQGQALH PLPKADRQWK
     GQFSIKQRSY TLRQLRDGWL YVYDETAKTL HEYEVVGCKL TKIDWSDDEA NKPTHERGSK
     GESKSCLLYP AQHTLSIGYA HQRWTWRVCE HMRSNTSSRH AVMRKVSLKQ FESNGTHPHA
     HFAQYLEDYV ADIGTPAEQD IFKDTCTPSL PVEKSEEAVK GTEFKFVADK AVVSSSDYLQ
     DLPEQNCGLF VALNDPLADV SDLFVTFTTQ VAKRTKAIGD ETQQHKMQMA ELTRTLGRIR
     LEEKEIPDFV KQDPIRILEL ERAITEYCAT AKLAEIESHH LASEGHSPSG NYALMQQQAE
     QKLAELKTLY RFEPTSAQMR KWRKKDNSFI DEVRWADLDN FLVEHYTELK GLDEQIKQHY
     AQFMSAFNQL GLDPLLFGMD NQDEVQQAYL LALTSQFLVV VTQVNHDEKS LEILKKDLSF
     DSPKNLMALA STGFSLQANQ AINNHIQGFS TAFLSTSNPS DMVAFATAIA NWDTFTGDER
     IQEKAWFKRW IEPAQSSFGA LQKAVANQAK ESWQAVMELL FPYQNQPKGG TPSLLANLRL
     LLVESLVREE AVLQHNPKYA AELKQFETKL NAILQEMNDA LELKPGNVSP KNHQIATAQS
     AQRKLGQLLS SELPMMLTLK NQAAMNTFQQ SVNEKLSALS KNVKTSSASV SQKLGGLGGL
     LFALNLWNTM TVLENIRYKV AQYPSWNPFK NPALGEAIYA TGNTIVVAGA ISAGRAWVTI
     AEQGLLDRTL KNALNTTKVL GTKDALKTFA KSIALVATVG MIASALETWE SWGKFNDSSK
     TDLERFGYLL KAGATGAQGI IFYIQFFTLL GSGIGGPSIA AISAGWMLAG FAVIGIVYLI
     GVILTNVFKR SELEIWLSKS TWGKESAHWP VGKELTELEH LLHRPSLRLS QVTQRKAAQW
     MDSGSLQWQL ELTLPDYLKG QTIGLQITRL PAQPAYYQPQ REAVTPILIN EQQGKWSIED
     NQPVYRITLG GSEKDTVGVC VALPLRWGKE LSLKFYASGT RAGELDLQSA EANDIATRNL
     VVGKG