VAT1_MOUSE
ID VAT1_MOUSE Reviewed; 406 AA.
AC Q62465; Q8R3G0; Q8R3T8;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 27-SEP-2004, sequence version 3.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Synaptic vesicle membrane protein VAT-1 homolog;
DE EC=1.-.-.-;
GN Name=Vat1; Synonyms=Vat-1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II, and FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 105-209, PROTEIN SEQUENCE OF 239-258;
RP 264-308; 369-378; 386-390 AND 396-404, AND FUNCTION.
RX PubMed=9581869;
RX DOI=10.1002/(sici)1097-4644(19980601)69:3<304::aid-jcb8>3.0.co;2-v;
RA Hayess K., Kraft R., Sachsinger J., Janke J., Beckmann G., Rohde K.,
RA Jandrig B., Benndorf R.;
RT "Mammalian protein homologous to VAT-1 of Torpedo californica: isolation
RT from Ehrlich ascites tumor cells, biochemical characterization, and
RT organization of its gene.";
RL J. Cell. Biochem. 69:304-315(1998).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas,
RC Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Plays a part in calcium-regulated keratinocyte activation in
CC epidermal repair mechanisms. Has no effect on cell proliferation (By
CC similarity). Possesses ATPase activity. Negatively regulates
CC mitochondrial fusion in cooperation with mitofusin proteins (MFN1-2)
CC (By similarity). {ECO:0000250, ECO:0000269|PubMed:9581869}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Mitochondrion outer membrane;
CC Peripheral membrane protein. Note=The majority is localized in the
CC cytoplasm a small amount is associated with mitochondria.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the zinc-containing alcohol dehydrogenase
CC family. Quinone oxidoreductase subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK034795; BAC28833.1; -; mRNA.
DR EMBL; BC024586; AAH24586.1; -; mRNA.
DR EMBL; BC025496; AAH25496.1; -; mRNA.
DR EMBL; X95562; CAA64807.1; -; mRNA.
DR CCDS; CCDS25472.1; -.
DR RefSeq; NP_036167.1; NM_012037.2.
DR AlphaFoldDB; Q62465; -.
DR SMR; Q62465; -.
DR BioGRID; 205084; 12.
DR IntAct; Q62465; 4.
DR STRING; 10090.ENSMUSP00000048350; -.
DR iPTMnet; Q62465; -.
DR PhosphoSitePlus; Q62465; -.
DR SwissPalm; Q62465; -.
DR EPD; Q62465; -.
DR jPOST; Q62465; -.
DR MaxQB; Q62465; -.
DR PaxDb; Q62465; -.
DR PRIDE; Q62465; -.
DR ProteomicsDB; 297572; -.
DR TopDownProteomics; Q62465; -.
DR Antibodypedia; 29494; 90 antibodies from 25 providers.
DR DNASU; 26949; -.
DR Ensembl; ENSMUST00000040430; ENSMUSP00000048350; ENSMUSG00000034993.
DR GeneID; 26949; -.
DR KEGG; mmu:26949; -.
DR UCSC; uc007loy.1; mouse.
DR CTD; 10493; -.
DR MGI; MGI:1349450; Vat1.
DR VEuPathDB; HostDB:ENSMUSG00000034993; -.
DR eggNOG; KOG1197; Eukaryota.
DR GeneTree; ENSGT00940000157579; -.
DR HOGENOM; CLU_026673_3_1_1; -.
DR InParanoid; Q62465; -.
DR OMA; MQYVTAW; -.
DR OrthoDB; 1085436at2759; -.
DR PhylomeDB; Q62465; -.
DR TreeFam; TF314255; -.
DR Reactome; R-MMU-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 26949; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Vat1; mouse.
DR PRO; PR:Q62465; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q62465; protein.
DR Bgee; ENSMUSG00000034993; Expressed in lip and 230 other tissues.
DR ExpressionAtlas; Q62465; baseline and differential.
DR Genevisible; Q62465; MM.
DR GO; GO:0005741; C:mitochondrial outer membrane; ISS:UniProtKB.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0010637; P:negative regulation of mitochondrial fusion; ISS:UniProtKB.
DR InterPro; IPR013154; ADH_N.
DR InterPro; IPR011032; GroES-like_sf.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020843; PKS_ER.
DR InterPro; IPR002364; Quin_OxRdtase/zeta-crystal_CS.
DR Pfam; PF08240; ADH_N; 1.
DR SMART; SM00829; PKS_ER; 1.
DR SUPFAM; SSF50129; SSF50129; 1.
DR SUPFAM; SSF51735; SSF51735; 1.
DR PROSITE; PS01162; QOR_ZETA_CRYSTAL; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Direct protein sequencing; Membrane; Mitochondrion;
KW Mitochondrion outer membrane; Oxidoreductase; Phosphoprotein;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q99536"
FT CHAIN 2..406
FT /note="Synaptic vesicle membrane protein VAT-1 homolog"
FT /id="PRO_0000160919"
FT REGION 1..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 39..54
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000250|UniProtKB:Q99536"
FT MOD_RES 2
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q3MIE4"
FT MOD_RES 33
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q99536"
FT MOD_RES 42
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q99536"
SQ SEQUENCE 406 AA; 43097 MW; 3F704C3667C21E37 CRC64;
MSAEREAAEA ATVAAATEAG AETGTGAGEG APSQPPTVEV ASDPQPPPAP EASASASAPP
LRCLVLTGFG GYDKVKLQSR PAVPPAPGPG QLTLRVRACG LNFADLMGRQ GLYDRLPPLP
VTPGMEGAGV VVAVGEGVGD RKAGDRVMVL NRSGMWQEEV TVPSAQTFLM PEAMTFEEAA
ALLVNYITAY MVLFDFGNLR PGHSVLVHMA AGGVGMAALQ LCRTVENVTV FGTASASKHE
VLKENGVTHP IDYHTTDYVD EIKKISPKGV DIVMDPLGGS DTAKGYHLLK PMGKVVTYGM
ANLLTGPKRN LMAMARTWWN QFSVTALQLL QANRAVCGFH LGYLDGEVEL VNSVVTRLVA
LYNQGHIKPR IDSVWPFEKV ADAMKQMQEK KNIGKVLLVP GPEKET
