ID   VATA_STAAU              Reviewed;         219 AA.
AC   P26839;
DT   01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 2.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Virginiamycin A acetyltransferase;
DE            EC=2.3.1.-;
GN   Name=vat;
OS   Staphylococcus aureus.
OG   Plasmid pIP630.
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=1280;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8344533; DOI=10.1016/0378-1119(93)90350-c;
RA   Allignet J., Loncle V., Simenel C., Delpierre M., el Solh N.;
RT   "Sequence of a staphylococcal gene, vat, encoding an acetyltransferase
RT   inactivating the A-type compounds of virginiamycin-like antibiotics.";
RL   Gene 130:91-98(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-112.
RX   PubMed=3149758; DOI=10.1016/0147-619x(88)90034-0;
RA   Allignet J., Loncle V., Mazodier P., El Solh N.;
RT   "Nucleotide sequence of a staphylococcal plasmid gene, vgb, encoding a
RT   hydrolase inactivating the B components of virginiamycin-like
RT   antibiotics.";
RL   Plasmid 20:271-275(1988).
CC   -!- FUNCTION: Inactivates the A compounds of virginiamycin-like
CC       antibiotics, thus providing resistance to these antibiotics.
CC   -!- SIMILARITY: Belongs to the transferase hexapeptide repeat family.
CC       {ECO:0000305}.
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DR   EMBL; L07778; AAA26683.1; -; Genomic_DNA.
DR   EMBL; M36022; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; JN0822; JN0822.
DR   RefSeq; WP_032489635.1; NG_048537.1.
DR   PDB; 4HUR; X-ray; 2.15 A; A/B/C=1-219.
DR   PDB; 4HUS; X-ray; 2.36 A; A/B/C=1-219.
DR   PDB; 4MYO; X-ray; 2.70 A; A/B/C=7-219.
DR   PDB; 6X3C; X-ray; 3.05 A; A/B/C/D/E/F=7-213.
DR   PDB; 6X3J; X-ray; 2.70 A; A/B/C/D/E/F=7-215.
DR   PDBsum; 4HUR; -.
DR   PDBsum; 4HUS; -.
DR   PDBsum; 4MYO; -.
DR   PDBsum; 6X3C; -.
DR   PDBsum; 6X3J; -.
DR   AlphaFoldDB; P26839; -.
DR   SMR; P26839; -.
DR   KEGG; ag:AAA26683; -.
DR   GO; GO:0016746; F:acyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR001451; Hexapep.
DR   InterPro; IPR018357; Hexapep_transf_CS.
DR   InterPro; IPR011004; Trimer_LpxA-like_sf.
DR   Pfam; PF00132; Hexapep; 1.
DR   SUPFAM; SSF51161; SSF51161; 1.
DR   PROSITE; PS00101; HEXAPEP_TRANSFERASES; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Acyltransferase; Antibiotic resistance; Plasmid; Repeat;
KW   Transferase.
FT   CHAIN           1..219
FT                   /note="Virginiamycin A acetyltransferase"
FT                   /id="PRO_0000068664"
FT   ACT_SITE        87
FT                   /evidence="ECO:0000250"
FT   CONFLICT        1
FT                   /note="M -> L (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   STRAND          21..26
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   HELIX           27..29
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   STRAND          35..37
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   STRAND          42..44
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   STRAND          46..48
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   HELIX           51..54
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   STRAND          55..57
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   TURN            60..62
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   STRAND          66..68
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   STRAND          79..81
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   HELIX           83..85
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   HELIX           96..99
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   HELIX           103..106
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   HELIX           110..112
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   STRAND          119..121
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   STRAND          159..162
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   TURN            163..166
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   STRAND          167..173
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   HELIX           175..184
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   HELIX           186..188
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   HELIX           191..203
FT                   /evidence="ECO:0007829|PDB:4HUR"
FT   HELIX           206..215
FT                   /evidence="ECO:0007829|PDB:4HUR"
SQ   SEQUENCE   219 AA;  24932 MW;  CDAFD846D0727207 CRC64;
     MNLNNDHGPD PENILPIKGN RNLQFIKPTI TNENILVGEY SYYDSKRGES FEDQVLYHYE
     VIGDKLIIGR FCSIGPGTTF IMNGANHRMD GSTYPFHLFR MGWEKYMPSL KDLPLKGDIE
     IGNDVWIGRD VTIMPGVKIG DGAIIAAEAV VTKNVAPYSI VGGNPLKFIR KRFSDGVIEE
     WLALQWWNLD MKIINENLPF IINGDIEMLK RKRKLLDDT