VATA_THET8
ID VATA_THET8 Reviewed; 578 AA.
AC Q56403; Q5SIU1;
DT 24-JAN-2001, integrated into UniProtKB/Swiss-Prot.
DT 20-JUN-2003, sequence version 2.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=V-type ATP synthase alpha chain;
DE EC=7.1.2.2;
DE AltName: Full=V-ATPase subunit A;
GN Name=atpA; OrderedLocusNames=TTHA1273;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1836357; DOI=10.1016/0005-2728(91)90003-7;
RA Tsutsumi S., Denda K., Yokoyama K., Oshima T., Date T., Yoshida M.;
RT "Molecular cloning of genes encoding major two subunits of a eubacterial V-
RT type ATPase from Thermus thermophilus.";
RL Biochim. Biophys. Acta 1098:13-20(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10788522; DOI=10.1074/jbc.275.18.13955;
RA Yokoyama K., Ohkuma S., Taguchi H., Yasunaga T., Wakabayashi T.,
RA Yoshida M.;
RT "V-type H+-ATPase/synthase from a thermophilic eubacterium, Thermus
RT thermophilus. Subunit structure and operon.";
RL J. Biol. Chem. 275:13955-13961(2000).
RN [3]
RP SEQUENCE REVISION TO C-TERMINUS.
RA Yokoyama K., Ohkuma S., Taguchi H., Yasunaga T., Wakabayashi T.,
RA Yoshida M.;
RL Submitted (NOV-2002) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Produces ATP from ADP in the presence of a proton gradient
CC across the membrane. The V-type alpha chain is a catalytic subunit.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + 4 H(+)(in) + H2O = ADP + 5 H(+)(out) + phosphate;
CC Xref=Rhea:RHEA:57720, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=7.1.2.2;
CC -!- INTERACTION:
CC Q56403; Q56404: atpB; NbExp=2; IntAct=EBI-7260009, EBI-7260036;
CC -!- SIMILARITY: Belongs to the ATPase alpha/beta chains family.
CC {ECO:0000305}.
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DR EMBL; X63855; CAA45340.1; -; Genomic_DNA.
DR EMBL; D63799; BAA09873.2; -; Genomic_DNA.
DR EMBL; AP008226; BAD71096.1; -; Genomic_DNA.
DR RefSeq; WP_011228560.1; NC_006461.1.
DR RefSeq; YP_144539.1; NC_006461.1.
DR PDB; 3A5C; X-ray; 4.51 A; A/B/C/I/J/K=1-578.
DR PDB; 3A5D; X-ray; 4.80 A; A/B/C/I/J/K=1-578.
DR PDB; 3GQB; X-ray; 2.80 A; A/C=1-578.
DR PDB; 3J0J; EM; 9.70 A; A/B/C=1-578.
DR PDB; 3W3A; X-ray; 3.90 A; A/B/C/I/J/K=1-577.
DR PDB; 5GAR; EM; 6.40 A; A/B/C=1-577.
DR PDB; 5GAS; EM; 9.50 A; A/B/C=1-577.
DR PDB; 5TSJ; EM; 8.70 A; A/B/C=1-577.
DR PDB; 5Y5X; EM; 5.00 A; A/B/C=1-578.
DR PDB; 5Y5Y; EM; 4.70 A; A/B/C=1-578.
DR PDB; 5Y5Z; EM; 6.70 A; A/B/C=1-578.
DR PDB; 5Y60; EM; 7.50 A; A/B/C=1-578.
DR PDB; 6LY8; EM; 3.50 A; A/B/C=1-578.
DR PDB; 6QUM; EM; 3.25 A; A/B/C=1-578.
DR PDB; 6R0W; EM; 3.60 A; A/B/C=1-578.
DR PDB; 6R0Y; EM; 3.90 A; A/B/C=1-578.
DR PDB; 6R0Z; EM; 3.80 A; A/B/C=1-578.
DR PDB; 6R10; EM; 4.30 A; A/B/C=1-578.
DR PDBsum; 3A5C; -.
DR PDBsum; 3A5D; -.
DR PDBsum; 3GQB; -.
DR PDBsum; 3J0J; -.
DR PDBsum; 3W3A; -.
DR PDBsum; 5GAR; -.
DR PDBsum; 5GAS; -.
DR PDBsum; 5TSJ; -.
DR PDBsum; 5Y5X; -.
DR PDBsum; 5Y5Y; -.
DR PDBsum; 5Y5Z; -.
DR PDBsum; 5Y60; -.
DR PDBsum; 6LY8; -.
DR PDBsum; 6QUM; -.
DR PDBsum; 6R0W; -.
DR PDBsum; 6R0Y; -.
DR PDBsum; 6R0Z; -.
DR PDBsum; 6R10; -.
DR AlphaFoldDB; Q56403; -.
DR SMR; Q56403; -.
DR IntAct; Q56403; 4.
DR MINT; Q56403; -.
DR STRING; 300852.55772655; -.
DR TCDB; 3.A.2.2.1; the h(+)- or na(+)-translocating f-type, v-type and a-type atpase (f-atpase) superfamily.
DR EnsemblBacteria; BAD71096; BAD71096; BAD71096.
DR GeneID; 3169043; -.
DR KEGG; ttj:TTHA1273; -.
DR PATRIC; fig|300852.9.peg.1252; -.
DR eggNOG; COG1155; Bacteria.
DR HOGENOM; CLU_008162_3_1_0; -.
DR OMA; TAFVQVY; -.
DR PhylomeDB; Q56403; -.
DR EvolutionaryTrace; Q56403; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0045259; C:proton-transporting ATP synthase complex; IEA:UniProt.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0046933; F:proton-transporting ATP synthase activity, rotational mechanism; IEA:UniProtKB-UniRule.
DR GO; GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; IEA:UniProtKB-EC.
DR GO; GO:0042777; P:proton motive force-driven plasma membrane ATP synthesis; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.1140.10; -; 1.
DR Gene3D; 2.40.30.20; -; 1.
DR Gene3D; 3.40.50.300; -; 1.
DR HAMAP; MF_00309; ATP_synth_A_arch; 1.
DR InterPro; IPR031686; ATP-synth_a_Xtn.
DR InterPro; IPR023366; ATP_synth_asu-like_sf.
DR InterPro; IPR020003; ATPase_a/bsu_AS.
DR InterPro; IPR004100; ATPase_F1/V1/A1_a/bsu_N.
DR InterPro; IPR036121; ATPase_F1/V1/A1_a/bsu_N_sf.
DR InterPro; IPR000194; ATPase_F1/V1/A1_a/bsu_nucl-bd.
DR InterPro; IPR024034; ATPase_F1/V1_b/a_C.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR022878; V-ATPase_asu.
DR PANTHER; PTHR43607; PTHR43607; 1.
DR Pfam; PF00006; ATP-synt_ab; 1.
DR Pfam; PF02874; ATP-synt_ab_N; 1.
DR Pfam; PF16886; ATP-synt_ab_Xtn; 1.
DR SUPFAM; SSF50615; SSF50615; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR PROSITE; PS00152; ATPASE_ALPHA_BETA; 1.
PE 1: Evidence at protein level;
KW 3D-structure; ATP synthesis; ATP-binding; Hydrogen ion transport;
KW Ion transport; Nucleotide-binding; Reference proteome; Translocase;
KW Transport.
FT CHAIN 1..578
FT /note="V-type ATP synthase alpha chain"
FT /id="PRO_0000144619"
FT BINDING 228..235
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT CONFLICT 577..578
FT /note="LA -> GLKGERWTF (in Ref. 1; CAA45340)"
FT /evidence="ECO:0000305"
FT STRAND 3..10
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 13..18
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 26..30
FT /evidence="ECO:0007829|PDB:3GQB"
FT TURN 31..34
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 35..43
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 46..53
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 63..76
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 80..84
FT /evidence="ECO:0007829|PDB:6QUM"
FT HELIX 91..98
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 113..115
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 117..121
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 134..140
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 143..148
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 155..160
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 163..166
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 168..170
FT /evidence="ECO:0007829|PDB:6LY8"
FT STRAND 172..175
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 185..188
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 196..199
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 203..205
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 210..213
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 223..226
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 230..232
FT /evidence="ECO:0007829|PDB:6LY8"
FT HELIX 234..244
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 248..257
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 259..266
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 269..271
FT /evidence="ECO:0007829|PDB:3GQB"
FT TURN 275..277
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 278..281
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 282..284
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 285..291
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 292..294
FT /evidence="ECO:0007829|PDB:6LY8"
FT HELIX 296..315
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 319..322
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 327..340
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 347..350
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 354..362
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 366..369
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 375..383
FT /evidence="ECO:0007829|PDB:3GQB"
FT TURN 387..389
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 394..400
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 404..406
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 411..416
FT /evidence="ECO:0007829|PDB:3GQB"
FT TURN 424..426
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 428..431
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 432..442
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 447..462
FT /evidence="ECO:0007829|PDB:3GQB"
FT TURN 463..472
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 473..475
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 478..494
FT /evidence="ECO:0007829|PDB:3GQB"
FT STRAND 499..501
FT /evidence="ECO:0007829|PDB:3GQB"
FT TURN 502..505
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 509..530
FT /evidence="ECO:0007829|PDB:3GQB"
FT TURN 531..533
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 536..539
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 545..549
FT /evidence="ECO:0007829|PDB:3GQB"
FT TURN 551..553
FT /evidence="ECO:0007829|PDB:6QUM"
FT STRAND 556..558
FT /evidence="ECO:0007829|PDB:3GQB"
FT HELIX 559..576
FT /evidence="ECO:0007829|PDB:3GQB"
SQ SEQUENCE 578 AA; 63632 MW; B22A91ECA7B89970 CRC64;
MIQGVIQKIA GPAVIAKGML GARMYDICKV GEEGLVGEII RLDGDTAFVQ VYEDTSGLKV
GEPVVSTGLP LAVELGPGML NGIYDGIQRP LERIREKTGI YITRGVVVHA LDREKKWAWT
PMVKPGDEVR GGMVLGTVPE FGFTHKILVP PDVRGRVKEV KPAGEYTVEE PVVVLEDGTE
LKMYHTWPVR RARPVQRKLD PNTPFLTGMR ILDVLFPVAM GGTAAIPGPF GSGKTVTQQS
LAKWSNADVV VYVGCGERGN EMTDVLVEFP ELTDPKTGGP LMHRTVLIAN TSNMPVAARE
ASIYVGVTIA EYFRDQGFSV ALMADSTSRW AEALREISSR LEEMPAEEGY PPYLAARLAA
FYERAGKVIT LGGEEGAVTI VGAVSPPGGD MSEPVTQSTL RIVGAFWRLD ASLAFRRHFP
AINWNGSYSL FTSALDPWYR ENVAEDYPEL RDAISELLQR EAGLQEIVQL VGPDALQDAE
RLVIEVGRII REDFLQQNAY HEVDAYCSMK KAYGIMKMIL AFYKEAEAAI KRGVSIDEIL
QLPVLERIGR ARYVSEEEFP AYFEEAMKEI QGAFKALA
