CAHM3_MOUSE
ID CAHM3_MOUSE Reviewed; 347 AA.
AC J3QMI4;
DT 26-FEB-2020, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=Calcium homeostasis modulator protein 3 {ECO:0000303|PubMed:29681531};
GN Name=Calhm3 {ECO:0000312|MGI:MGI:3645665};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, INTERACTION WITH CALHM1, SUBCELLULAR
RP LOCATION, AND DISRUPTION PHENOTYPE.
RC STRAIN=C57BL/6J; TISSUE=Tongue epithelium;
RX PubMed=29681531; DOI=10.1016/j.neuron.2018.03.043;
RA Ma Z., Taruno A., Ohmoto M., Jyotaki M., Lim J.C., Miyazaki H., Niisato N.,
RA Marunaka Y., Lee R.J., Hoff H., Payne R., Demuro A., Parker I.,
RA Mitchell C.H., Henao-Mejia J., Tanis J.E., Matsumoto I., Tordoff M.G.,
RA Foskett J.K.;
RT "CALHM3 Is Essential for Rapid Ion Channel-Mediated Purinergic
RT Neurotransmission of GPCR-Mediated Tastes.";
RL Neuron 98:547-561(2018).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND MUTAGENESIS OF 76-LEU-MET-77;
RP TYR-219 AND LEU-222.
RX PubMed=30804437; DOI=10.1038/s41598-019-39593-5;
RA Kashio M., Wei-Qi G., Ohsaki Y., Kido M.A., Taruno A.;
RT "CALHM1/CALHM3 channel is intrinsically sorted to the basolateral membrane
RT of epithelial cells including taste cells.";
RL Sci. Rep. 9:2681-2681(2019).
CC -!- FUNCTION: Pore-forming subunit of a voltage-gated ion channel, also
CC permeable to larger molecules including ATP (PubMed:29681531). Together
CC with CALHM1, forms a fast-activating voltage-gated ATP-release channel
CC in type II taste bud cells (TBCs) (PubMed:29681531). CALHM1-CALHM3-
CC mediated ATP released acts as a neurotransmitter to gustatory neurons
CC in response to GPCR-mediated tastes, including sweet, bitter and umami
CC substances (PubMed:29681531). {ECO:0000269|PubMed:29681531}.
CC -!- SUBUNIT: Associates with CALHM1 as a pore-forming subunit in an hetero-
CC hexameric channel complex. {ECO:0000269|PubMed:29681531}.
CC -!- SUBCELLULAR LOCATION: Basolateral cell membrane
CC {ECO:0000269|PubMed:30804437, ECO:0000305|PubMed:29681531}; Multi-pass
CC membrane protein {ECO:0000255}. Note=Localizes to the basolateral
CC membrane of epithelial cells including taste cells.
CC {ECO:0000269|PubMed:30804437}.
CC -!- TISSUE SPECIFICITY: Expressed in taste bud cells.
CC {ECO:0000269|PubMed:30804437}.
CC -!- DISRUPTION PHENOTYPE: Genetic deletion eliminates voltage-gated
CC nonselective currents and taste-evoked ATP release in type II TBCs
CC without affecting cell excitability or diminishing Calhm1 expression,
CC and results in the loss of responses to sweet, umami and bitter tastes.
CC {ECO:0000269|PubMed:29681531}.
CC -!- SIMILARITY: Belongs to the CALHM family. {ECO:0000305}.
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DR EMBL; LC270871; BBB16320.1; -; mRNA.
DR EMBL; AC124724; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; J3QMI4; -.
DR SMR; J3QMI4; -.
DR STRING; 10090.ENSMUSP00000136302; -.
DR PhosphoSitePlus; J3QMI4; -.
DR SwissPalm; J3QMI4; -.
DR PaxDb; J3QMI4; -.
DR PRIDE; J3QMI4; -.
DR Antibodypedia; 3118; 113 antibodies from 20 providers.
DR Ensembl; ENSMUST00000178630; ENSMUSP00000136302; ENSMUSG00000094219.
DR MGI; MGI:3645665; Calhm3.
DR VEuPathDB; HostDB:ENSMUSG00000094219; -.
DR eggNOG; ENOG502QRUK; Eukaryota.
DR GeneTree; ENSGT01030000234610; -.
DR HOGENOM; CLU_069286_0_0_1; -.
DR InParanoid; J3QMI4; -.
DR OMA; RCWSQAL; -.
DR PhylomeDB; J3QMI4; -.
DR TreeFam; TF329085; -.
DR PRO; PR:J3QMI4; -.
DR Proteomes; UP000000589; Chromosome 19.
DR RNAct; J3QMI4; protein.
DR Bgee; ENSMUSG00000094219; Expressed in islet of Langerhans and 3 other tissues.
DR GO; GO:0016323; C:basolateral plasma membrane; IDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; TAS:Reactome.
DR GO; GO:0005261; F:cation channel activity; IBA:GO_Central.
DR GO; GO:0005244; F:voltage-gated ion channel activity; IDA:UniProtKB.
DR GO; GO:0015867; P:ATP transport; IDA:UniProtKB.
DR GO; GO:0051291; P:protein heterooligomerization; IDA:UniProtKB.
DR GO; GO:0050896; P:response to stimulus; IEA:UniProtKB-KW.
DR GO; GO:0050909; P:sensory perception of taste; IDA:UniProtKB.
DR InterPro; IPR029569; CALHM.
DR InterPro; IPR029571; CALHM3.
DR PANTHER; PTHR32261; PTHR32261; 1.
DR PANTHER; PTHR32261:SF7; PTHR32261:SF7; 1.
DR Pfam; PF14798; Ca_hom_mod; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Ion channel; Ion transport; Membrane; Reference proteome;
KW Sensory transduction; Taste; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..347
FT /note="Calcium homeostasis modulator protein 3"
FT /id="PRO_0000449205"
FT TRANSMEM 17..37
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 50..70
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 108..128
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 265..290
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 274..289
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 76..77
FT /note="LM->AA: Affects basolateral membrane sorting in
FT vitro but not in vivo; when associated with A-219 and A-
FT 222."
FT /evidence="ECO:0000269|PubMed:30804437"
FT MUTAGEN 219
FT /note="Y->A: Affects basolateral membrane sorting in vitro
FT but not in vivo; when associated with 76-A-A-77 and A-222."
FT /evidence="ECO:0000269|PubMed:30804437"
FT MUTAGEN 222
FT /note="L->A: Affects basolateral membrane sorting in vitro
FT but not in vivo; when associated with 76-A-A-77 and A-219."
FT /evidence="ECO:0000269|PubMed:30804437"
SQ SEQUENCE 347 AA; 39328 MW; 670DC19596E4CF24 CRC64;
MDRFRMLFQH LQSSSESVMN GICLLLAAVT VKIYSSLDFN CPCLERYNAL YGLGLLLTPP
LALFLCGLLV NRQSVLMVEE WRRPAGHRRK DLGIIRYMCS SVLQRALAAP LVWILLALLD
GKCFVCAFSN SVDPEKFLDF ANMTPRQVQL FLAKVPCKED ELVKNSPARK AVSRYLRCLS
QAIGWSITLL VIVVAFLARC LRPCFDQTVF LQRRYWSNYM DLEQKLFDET CCEHARDFAH
RCVLHFFANM QSELRALGLR RDPAGGIPES QESSEPPELR EDRDSGNGKA HLRAISSREQ
VDQLLSTWYS SKPPLDLAAS PRRWGPGLNH RAPIAAPGTK LCHQLNV
