ID   CAHS2_RAMVA             Reviewed;         216 AA.
AC   J7MDG6;
DT   07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2012, sequence version 1.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=Cytosolic-abundant heat soluble protein 2 {ECO:0000303|PubMed:22937162};
DE            Short=CAHS2 {ECO:0000303|PubMed:22937162};
DE   AltName: Full=Tardigrade-specific intrinsically disordered protein CAHS2 {ECO:0000305};
DE            Short=TDP CAHS2 {ECO:0000305};
GN   Name=CAHS2 {ECO:0000303|PubMed:22937162}; ORFNames=RvY_00946;
OS   Ramazzottius varieornatus (Water bear) (Tardigrade).
OC   Eukaryota; Metazoa; Ecdysozoa; Tardigrada; Eutardigrada; Parachela;
OC   Hypsibioidea; Ramazzottiidae; Ramazzottius.
OX   NCBI_TaxID=947166;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, AND
RP   DOMAIN.
RC   STRAIN=YOKOZUNA-1;
RX   PubMed=22937162; DOI=10.1371/journal.pone.0044209;
RA   Yamaguchi A., Tanaka S., Yamaguchi S., Kuwahara H., Takamura C.,
RA   Imajoh-Ohmi S., Horikawa D.D., Toyoda A., Katayama T., Arakawa K.,
RA   Fujiyama A., Kubo T., Kunieda T.;
RT   "Two novel heat-soluble protein families abundantly expressed in an
RT   anhydrobiotic tardigrade.";
RL   PLoS ONE 7:E44209-E44209(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YOKOZUNA-1;
RX   PubMed=27649274; DOI=10.1038/ncomms12808;
RA   Hashimoto T., Horikawa D.D., Saito Y., Kuwahara H., Kozuka-Hata H.,
RA   Shin-I T., Minakuchi Y., Ohishi K., Motoyama A., Aizu T., Enomoto A.,
RA   Kondo K., Tanaka S., Hara Y., Koshikawa S., Sagara H., Miura T.,
RA   Yokobori S., Miyagawa K., Suzuki Y., Kubo T., Oyama M., Kohara Y.,
RA   Fujiyama A., Arakawa K., Katayama T., Toyoda A., Kunieda T.;
RT   "Extremotolerant tardigrade genome and improved radiotolerance of human
RT   cultured cells by tardigrade-unique protein.";
RL   Nat. Commun. 7:12808-12808(2016).
RN   [3]
RP   FUNCTION.
RX   PubMed=33545053; DOI=10.1016/j.molcel.2020.12.007;
RA   Arakawa K., Numata K.;
RT   "Reconsidering the 'glass transition' hypothesis of intrinsically
RT   unstructured CAHS proteins in desiccation tolerance of tardigrades.";
RL   Mol. Cell 81:409-410(2021).
CC   -!- FUNCTION: CAHS proteins are cytosolic heat soluble proteins that seem
CC       to contribute to the anhydrobiosis in tardigrades, but their specific
CC       mechanisms are yet to be identified (PubMed:22937162, PubMed:33545053).
CC       It is possible that protection during anhydrobiosis might occur via the
CC       stabilization of vitrifying small molecules such as sugars, but not via
CC       the direct glass transition of CAHS proteins themselves (Probable).
CC       {ECO:0000269|PubMed:22937162, ECO:0000269|PubMed:33545053,
CC       ECO:0000305|PubMed:33545053}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:J7M3T1}.
CC   -!- DOMAIN: CAHS proteins contain 2 repeats of 19-mer peptides designated
CC       as CAHS-motifs that comprise each two octapeptides connected by a
CC       tripeptide (PubMed:27649274). {ECO:0000305|PubMed:22937162}.
CC   -!- MISCELLANEOUS: Trehalose, a disaccharide essential for several
CC       organisms to survive drying, is detected at low levels or not at all in
CC       some tardigrade species, indicating that tardigrades possess
CC       potentially novel mechanisms for surviving desiccation involving
CC       tardigrade-specific intrinsically disordered proteins (TDPs) (By
CC       similarity). {ECO:0000250|UniProtKB:P0CU44}.
CC   -!- SIMILARITY: Belongs to the Cytosolic-abundant heat soluble protein
CC       (CAHS) family. {ECO:0000305}.
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DR   EMBL; AB650500; BAM37959.1; -; mRNA.
DR   EMBL; BDGG01000001; GAU88204.1; -; Genomic_DNA.
DR   AlphaFoldDB; J7MDG6; -.
DR   SMR; J7MDG6; -.
DR   Proteomes; UP000186922; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; Reference proteome; Repeat; Stress response.
FT   CHAIN           1..216
FT                   /note="Cytosolic-abundant heat soluble protein 2"
FT                   /id="PRO_0000440188"
FT   REGION          1..34
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          66..91
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          115..133
FT                   /note="CAHS motif 1"
FT                   /evidence="ECO:0000305|PubMed:22937162"
FT   REGION          152..170
FT                   /note="CAHS motif 2"
FT                   /evidence="ECO:0000305|PubMed:22937162"
FT   COILED          81..180
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   216 AA;  24587 MW;  053A64342ED79408 CRC64;
     MSRDQGSTEY DANQRQEQHQ EQHNTSYTHT DVRTNIPNIP APFISTGVSG LGQQLVGEGF
     TASAARISGQ SSETHVQMTP EMEAEARKDR ERYERELQAI NERHQRDIEG KTEAYRKQAE
     QEAERLRKEL EKQHQRDIEF RKSLVQGTIE NQKRQVELEA QLAKRELDRE ARLATQALDQ
     SKMATDVQVN FDSAVGHTVS GATTVSQSEK VTQSKH