ID   CAHS3_RAMVA             Reviewed;         303 AA.
AC   J7M3T1;
DT   07-JUN-2017, integrated into UniProtKB/Swiss-Prot.
DT   31-OCT-2012, sequence version 1.
DT   25-MAY-2022, entry version 15.
DE   RecName: Full=Cytosolic-abundant heat soluble protein 3 {ECO:0000303|PubMed:22937162};
DE            Short=CAHS3 {ECO:0000303|PubMed:22937162};
DE   AltName: Full=Tardigrade-specific intrinsically disordered protein CAHS3 {ECO:0000305};
DE            Short=TDP CAHS3 {ECO:0000305};
GN   Name=CAHS3 {ECO:0000303|PubMed:22937162}; ORFNames=RvY_16236;
OS   Ramazzottius varieornatus (Water bear) (Tardigrade).
OC   Eukaryota; Metazoa; Ecdysozoa; Tardigrada; Eutardigrada; Parachela;
OC   Hypsibioidea; Ramazzottiidae; Ramazzottius.
OX   NCBI_TaxID=947166;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], IDENTIFICATION BY MASS SPECTROMETRY, FUNCTION,
RP   DOMAIN, AND SUBCELLULAR LOCATION.
RC   STRAIN=YOKOZUNA-1;
RX   PubMed=22937162; DOI=10.1371/journal.pone.0044209;
RA   Yamaguchi A., Tanaka S., Yamaguchi S., Kuwahara H., Takamura C.,
RA   Imajoh-Ohmi S., Horikawa D.D., Toyoda A., Katayama T., Arakawa K.,
RA   Fujiyama A., Kubo T., Kunieda T.;
RT   "Two novel heat-soluble protein families abundantly expressed in an
RT   anhydrobiotic tardigrade.";
RL   PLoS ONE 7:E44209-E44209(2012).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=YOKOZUNA-1;
RX   PubMed=27649274; DOI=10.1038/ncomms12808;
RA   Hashimoto T., Horikawa D.D., Saito Y., Kuwahara H., Kozuka-Hata H.,
RA   Shin-I T., Minakuchi Y., Ohishi K., Motoyama A., Aizu T., Enomoto A.,
RA   Kondo K., Tanaka S., Hara Y., Koshikawa S., Sagara H., Miura T.,
RA   Yokobori S., Miyagawa K., Suzuki Y., Kubo T., Oyama M., Kohara Y.,
RA   Fujiyama A., Arakawa K., Katayama T., Toyoda A., Kunieda T.;
RT   "Extremotolerant tardigrade genome and improved radiotolerance of human
RT   cultured cells by tardigrade-unique protein.";
RL   Nat. Commun. 7:12808-12808(2016).
RN   [3]
RP   FUNCTION.
RX   PubMed=33545053; DOI=10.1016/j.molcel.2020.12.007;
RA   Arakawa K., Numata K.;
RT   "Reconsidering the 'glass transition' hypothesis of intrinsically
RT   unstructured CAHS proteins in desiccation tolerance of tardigrades.";
RL   Mol. Cell 81:409-410(2021).
CC   -!- FUNCTION: CAHS proteins are cytosolic heat soluble proteins that seem
CC       to contribute to the anhydrobiosis in tardigrades, but their specific
CC       mechanisms are yet to be identified (PubMed:22937162, PubMed:33545053).
CC       It is possible that protection during anhydrobiosis might occur via the
CC       stabilization of vitrifying small molecules such as sugars, but not via
CC       the direct glass transition of CAHS proteins themselves (Probable).
CC       {ECO:0000269|PubMed:22937162, ECO:0000269|PubMed:33545053,
CC       ECO:0000305|PubMed:33545053}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22937162}.
CC   -!- DOMAIN: CAHS proteins contain 2 repeats of 19-mer peptides designated
CC       as CAHS-motifs that comprise each two octapeptides connected by a
CC       tripeptide (PubMed:27649274). {ECO:0000305|PubMed:22937162}.
CC   -!- SIMILARITY: Belongs to the Cytosolic-abundant heat soluble protein
CC       (CAHS) family. {ECO:0000305}.
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DR   EMBL; AB650501; BAM37960.1; -; mRNA.
DR   EMBL; BDGG01000013; GAV06214.1; -; Genomic_DNA.
DR   AlphaFoldDB; J7M3T1; -.
DR   SMR; J7M3T1; -.
DR   Proteomes; UP000186922; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
PE   1: Evidence at protein level;
KW   Coiled coil; Cytoplasm; Reference proteome; Repeat; Stress response.
FT   CHAIN           1..303
FT                   /note="Cytosolic-abundant heat soluble protein 3"
FT                   /id="PRO_0000440189"
FT   REGION          1..67
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          184..202
FT                   /note="CAHS motif 1"
FT                   /evidence="ECO:0000305|PubMed:22937162"
FT   REGION          221..239
FT                   /note="CAHS motif 2"
FT                   /evidence="ECO:0000305|PubMed:22937162"
FT   REGION          270..303
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          170..257
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        1..48
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        270..294
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   303 AA;  33128 MW;  557507F573CC446F CRC64;
     MSSRQNQQSS SQHSSSSQQG GQGGQGVQGS SSYSRTEVHT SSGGPTIGGA QRTVPVPPGS
     HSEVHEEREV IKHGTKTESE THVVTVPVTT FGSTNMESVR TGFTVTQDKN LTVAAPNIAA
     PIHSNLDLNL GGGARAEITA GTTVDLSKIQ RKDLGPEEYA RYKAKVEQLA RQDEQDAGMR
     AAQYREEVER DAELIRQILE RQHIRDLEFR KEMVENQVNR QEREIQLEAE YAMRALELER
     NAAKEALESA KAQTNVNVKV ESAIGTTVSK GAIQTSADKS STTKTGPTTV TQIKHTEQHT
     ERR