ID   VATE_NEUCR              Reviewed;         230 AA.
AC   Q01278; Q7RVK9; V5INX5;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 128.
DE   RecName: Full=V-type proton ATPase subunit E;
DE            Short=V-ATPase subunit E;
DE   AltName: Full=V-ATPase 26 kDa subunit;
DE   AltName: Full=Vacuolar proton pump subunit E;
GN   Name=vma-4 {ECO:0000303|PubMed:7619848}; ORFNames=NCU07446;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=74A;
RX   PubMed=7619848; DOI=10.1016/0005-2736(95)00108-f;
RA   Bowman E.J., Steinhardt A., Bowman B.J.;
RT   "Isolation of the vma-4 gene encoding the 26 kDa subunit of the Neurospora
RT   crassa vacuolar ATPase.";
RL   Biochim. Biophys. Acta 1237:95-98(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
CC   -!- FUNCTION: Subunit of the V1 complex of vacuolar(H+)-ATPase (V-ATPase),
CC       a multisubunit enzyme composed of a peripheral complex (V1) that
CC       hydrolyzes ATP and a membrane integral complex (V0) that translocates
CC       protons (By similarity). V-ATPase is responsible for acidifying and
CC       maintaining the pH of intracellular compartments (By similarity).
CC       {ECO:0000250|UniProtKB:P22203}.
CC   -!- SUBUNIT: V-ATPase is a heteromultimeric enzyme composed of a peripheral
CC       catalytic V1 complex (components A to H) attached to an integral
CC       membrane V0 proton pore complex (components: a, c, c', c'', d, e, f and
CC       VOA1). {ECO:0000250|UniProtKB:P22203}.
CC   -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250|UniProtKB:P22203};
CC       Peripheral membrane protein {ECO:0000250|UniProtKB:P22203}; Cytoplasmic
CC       side {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the V-ATPase E subunit family. {ECO:0000305}.
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DR   EMBL; U17641; AAA87901.1; -; mRNA.
DR   EMBL; CM002236; ESA43772.1; -; Genomic_DNA.
DR   RefSeq; XP_011393174.1; XM_011394872.1.
DR   AlphaFoldDB; Q01278; -.
DR   SMR; Q01278; -.
DR   STRING; 5141.EFNCRP00000007440; -.
DR   PRIDE; Q01278; -.
DR   EnsemblFungi; ESA43772; ESA43772; NCU07446.
DR   GeneID; 3880773; -.
DR   KEGG; ncr:NCU07446; -.
DR   VEuPathDB; FungiDB:NCU07446; -.
DR   InParanoid; Q01278; -.
DR   Proteomes; UP000001805; Chromosome 1, Linkage Group I.
DR   GO; GO:0000329; C:fungal-type vacuole membrane; IEA:EnsemblFungi.
DR   GO; GO:0000221; C:vacuolar proton-transporting V-type ATPase, V1 domain; ISS:UniProtKB.
DR   GO; GO:0046961; F:proton-transporting ATPase activity, rotational mechanism; IBA:GO_Central.
DR   Gene3D; 3.30.2320.30; -; 1.
DR   HAMAP; MF_00311; ATP_synth_E_arch; 1.
DR   InterPro; IPR038495; ATPase_E_C.
DR   InterPro; IPR002842; ATPase_V1_Esu.
DR   PANTHER; PTHR45715; PTHR45715; 1.
DR   Pfam; PF01991; vATP-synt_E; 1.
PE   2: Evidence at transcript level;
KW   Hydrogen ion transport; Ion transport; Membrane; Reference proteome;
KW   Transport; Vacuole.
FT   CHAIN           1..230
FT                   /note="V-type proton ATPase subunit E"
FT                   /id="PRO_0000117308"
SQ   SEQUENCE   230 AA;  25748 MW;  6A2C95F8267618ED CRC64;
     MSQVHALSDD QVGQELRKMT AFIKQEAEEK AREIQIKADE EFAIEKSKLV RQETDAIDSA
     YAKKFKQAQM SQQITRSTMA NKTRLRVLGA RQELLDEIFE AASAQLGQAT HDLGRYKDIL
     RDLILEGFYA MNEPELVIRA RQADYDAVRE AAGWASAQYK HKTDKDVKAT IDAENPVPEG
     SAGGIIIVGG NGKIDIDNTF EARLTLLKDS ALPAMRKALF GENPNRKFFD