CAIAP_DANRE
ID CAIAP_DANRE Reviewed; 744 AA.
AC Q5TYM7;
DT 11-DEC-2019, integrated into UniProtKB/Swiss-Prot.
DT 07-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=CARD- and ANK-domain containing inflammasome adapter protein {ECO:0000305};
GN Name=caiap {ECO:0000312|ZFIN:ZDB-GENE-041014-19};
GN Synonyms=si:dkey-86e18.2 {ECO:0000312|ZFIN:ZDB-GENE-041014-19};
OS Danio rerio (Zebrafish) (Brachydanio rerio).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC Danionidae; Danioninae; Danio.
OX NCBI_TaxID=7955 {ECO:0000312|Proteomes:UP000000437};
RN [1] {ECO:0000312|Proteomes:UP000000437}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Tuebingen {ECO:0000312|Proteomes:UP000000437};
RX PubMed=23594743; DOI=10.1038/nature12111;
RA Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT "The zebrafish reference genome sequence and its relationship to the human
RT genome.";
RL Nature 496:498-503(2013).
RN [2] {ECO:0000305}
RP FUNCTION, SUBUNIT, INTERACTION WITH CASPA, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION BY S.TYPHIMURIUM, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=29123523; DOI=10.3389/fimmu.2017.01375;
RA Tyrkalska S.D., Candel S., Perez-Oliva A.B., Valera A., Alcaraz-Perez F.,
RA Garcia-Moreno D., Cayuela M.L., Mulero V.;
RT "Identification of an Evolutionarily Conserved Ankyrin Domain-Containing
RT Protein, Caiap, Which Regulates Inflammasome-Dependent Resistance to
RT Bacterial Infection.";
RL Front. Immunol. 8:1375-1375(2017).
CC -!- FUNCTION: Probable inflammasome component which mediates inflammasome-
CC dependent resistance to bacterial infection by S.typhimurium through
CC association with activated caspa. {ECO:0000269|PubMed:29123523}.
CC -!- SUBUNIT: Self-associates (PubMed:29123523). Interacts with caspa
CC (PubMed:29123523). {ECO:0000269|PubMed:29123523}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:29123523}. Note=In
CC the cytoplasm, forms a ring around specks which are large protein
CC aggregates. {ECO:0000269|PubMed:29123523}.
CC -!- TISSUE SPECIFICITY: Expressed in kidney, heart and skin
CC (PubMed:29123523). Not expressed in muscle, ovary, gills, eyes or brain
CC (PubMed:29123523). {ECO:0000269|PubMed:29123523}.
CC -!- DEVELOPMENTAL STAGE: Highly expressed at fertilization.
CC {ECO:0000269|PubMed:29123523}.
CC -!- INDUCTION: Up-regulated in response to bacterial infection with
CC S.typhimurium (PubMed:29123523). Highly expressed in macrophages when
CC infected with S.typhimurium (PubMed:29123523).
CC {ECO:0000269|PubMed:29123523}.
CC -!- DISRUPTION PHENOTYPE: Morpholino knockdown results in reduced caspa
CC activity and impairs caspa activity in response to bacterial infection
CC with S.typhimurium (PubMed:29123523). In addition, larvae have
CC increased susceptibility to the bacterium S.typhimurium
CC (PubMed:29123523). {ECO:0000269|PubMed:29123523}.
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DR EMBL; CR392368; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_001020663.1; NM_001025492.1.
DR AlphaFoldDB; Q5TYM7; -.
DR SMR; Q5TYM7; -.
DR STRING; 7955.ENSDARP00000115713; -.
DR PaxDb; Q5TYM7; -.
DR Ensembl; ENSDART00000140250; ENSDARP00000115713; ENSDARG00000092758.
DR GeneID; 557416; -.
DR KEGG; dre:557416; -.
DR CTD; 557416; -.
DR ZFIN; ZDB-GENE-041014-19; caiap.
DR eggNOG; KOG4177; Eukaryota.
DR GeneTree; ENSGT00940000165489; -.
DR HOGENOM; CLU_000134_48_5_1; -.
DR InParanoid; Q5TYM7; -.
DR OMA; HLDIIRY; -.
DR OrthoDB; 1011028at2759; -.
DR PhylomeDB; Q5TYM7; -.
DR TreeFam; TF329520; -.
DR PRO; PR:Q5TYM7; -.
DR Proteomes; UP000000437; Genome assembly.
DR Proteomes; UP000814640; Chromosome 20.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0006954; P:inflammatory response; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IMP:ZFIN.
DR GO; GO:0042981; P:regulation of apoptotic process; IEA:InterPro.
DR Gene3D; 1.10.533.10; -; 1.
DR Gene3D; 1.25.40.20; -; 6.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR001315; CARD.
DR InterPro; IPR011029; DEATH-like_dom_sf.
DR Pfam; PF00023; Ank; 1.
DR Pfam; PF12796; Ank_2; 4.
DR Pfam; PF13637; Ank_4; 2.
DR Pfam; PF00619; CARD; 1.
DR PRINTS; PR01415; ANKYRIN.
DR SMART; SM00248; ANK; 16.
DR SUPFAM; SSF47986; SSF47986; 1.
DR SUPFAM; SSF48403; SSF48403; 2.
DR PROSITE; PS50297; ANK_REP_REGION; 1.
DR PROSITE; PS50088; ANK_REPEAT; 12.
DR PROSITE; PS50209; CARD; 1.
PE 1: Evidence at protein level;
KW ANK repeat; Cytoplasm; Immunity; Inflammatory response; Innate immunity;
KW Reference proteome; Repeat.
FT CHAIN 1..744
FT /note="CARD- and ANK-domain containing inflammasome adapter
FT protein"
FT /id="PRO_0000448788"
FT DOMAIN 7..79
FT /note="CARD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00046"
FT REPEAT 157..185
FT /note="ANK 1"
FT /evidence="ECO:0000255"
FT REPEAT 189..218
FT /note="ANK 2"
FT /evidence="ECO:0000255"
FT REPEAT 222..251
FT /note="ANK 3"
FT /evidence="ECO:0000255"
FT REPEAT 255..285
FT /note="ANK 4"
FT /evidence="ECO:0000255"
FT REPEAT 287..314
FT /note="ANK 5"
FT /evidence="ECO:0000255"
FT REPEAT 318..347
FT /note="ANK 6"
FT /evidence="ECO:0000255"
FT REPEAT 377..406
FT /note="ANK 7"
FT /evidence="ECO:0000255"
FT REPEAT 410..439
FT /note="ANK 8"
FT /evidence="ECO:0000255"
FT REPEAT 443..472
FT /note="ANK 9"
FT /evidence="ECO:0000255"
FT REPEAT 476..505
FT /note="ANK 10"
FT /evidence="ECO:0000255"
FT REPEAT 509..538
FT /note="ANK 11"
FT /evidence="ECO:0000255"
FT REPEAT 542..571
FT /note="ANK 12"
FT /evidence="ECO:0000255"
FT REPEAT 575..604
FT /note="ANK 13"
FT /evidence="ECO:0000255"
FT REPEAT 608..637
FT /note="ANK 14"
FT /evidence="ECO:0000255"
FT REPEAT 643..672
FT /note="ANK 15"
FT /evidence="ECO:0000255"
FT REPEAT 676..705
FT /note="ANK 16"
FT /evidence="ECO:0000255"
FT REGION 127..146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 744 AA; 80833 MW; 832BC6CD4BB33980 CRC64;
MGSTSFTNPY AIEVIQMKRS ELVSGILNPE DLLDLLIANG VLQPDIRVLV SDLTVREEKN
SRMLNILISR GERACRIFFY PCLKRAEPDL YQHMRTYVGG VNEGIRDARR QLIGYLLEKD
KQGLVKNSKP ERIHPKSTAN EPNNEPLNKQ ILKSESNHHD AILEAISSGD LYLLQELTKE
LDVNSVLSSN DTLLHHAAEY GKEAIVYFLL RQGAKLDLKD KEGRTALHRA AQRGHTAVAV
ALAKAGADIH ATDQTSKTPL HLAAQNGHEG CVKALVHEEK KSLKNQTTVL HMAAIEDNAT
LAEVLLRNGA LVDAQDGQRK TALHHAVRHG NEKTAAVLLK AGAQVDSRIV DAAFQLNRKS
LLSLFLQHVH ESMSQTEINA ALFKAVQRNL DAVVAALIEH GADVNSCNEL GYTPVLLAAE
LGNGEAFKVL VSKKAKLDER LPNQMSSLHL AVQSGSIQIA QILLHKGIDP NISGPKDQTP
LHLSASHNQP AMMALLLRVG AQLNPVTQDG FTPLHLASQN GHTEAVAQLL EAKADVHAKD
KQGRTALHWA AEQGEVAIIQ SLLAAGAYSN ASEREKKTPL HLAAAEGHTK AVSALLAGKA
KVGAKDMDGC SPLHYAARNG KERAGSVLLA SSKSKNVDDK NVWRRTALHL AAEHGHEALV
GILLENKAKI NALDNNKDTP LHCACKTGHL GTVQRLINWT NGERANLQAT NNVKKNALQV
AEAGDTEAHE NISTLLKKKM FLVK
