VAX2_MOUSE
ID VAX2_MOUSE Reviewed; 292 AA.
AC Q9WTP9;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1999, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Ventral anterior homeobox 2;
DE AltName: Full=Ventral retina homeodomain protein;
GN Name=Vax2; Synonyms=Vex;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=BALB/cJ;
RX PubMed=10421837; DOI=10.1046/j.1365-2443.1999.00257.x;
RA Ohsaki K., Morimitsu T., Ishida Y., Kominami R., Takahashi N.;
RT "Expression of the Vax family homeobox genes suggests multiple roles in eye
RT development.";
RL Genes Cells 4:267-276(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE.
RC TISSUE=Embryo;
RX PubMed=10485894; DOI=10.1073/pnas.96.19.10729;
RA Barbieri A.M., Lupo G., Bulfone A., Andreazzoli M., Mariani M.,
RA Fougerousse F., Consalez G.G., Borsani G., Beckmann J.S., Barsacchi G.,
RA Ballabio A., Banfi S.;
RT "A homeobox gene, vax2, controls the patterning of the eye dorsoventral
RT axis.";
RL Proc. Natl. Acad. Sci. U.S.A. 96:10729-10734(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RC TISSUE=Retina;
RX PubMed=10595508; DOI=10.1016/s0896-6273(00)81111-3;
RA Schulte D., Furukawa T., Peters M.A., Kozak C.A., Cepko C.L.;
RT "Misexpression of the Emx-related homeobox genes cVax and mVax2 ventralizes
RT the retina and perturbs the retinotectal map.";
RL Neuron 24:541-553(1999).
RN [4]
RP FUNCTION IN EYE DEVELOPMENT.
RX PubMed=11830578; DOI=10.1242/dev.129.3.797;
RA Mui S.H., Hindges R., O'Leary D.D., Lemke G., Bertuzzi S.;
RT "The homeodomain protein Vax2 patterns the dorsoventral and nasotemporal
RT axes of the eye.";
RL Development 129:797-804(2002).
RN [5]
RP FUNCTION.
RX PubMed=21856776; DOI=10.1101/gad.17227011;
RA Vacik T., Stubbs J.L., Lemke G.;
RT "A novel mechanism for the transcriptional regulation of Wnt signaling in
RT development.";
RL Genes Dev. 25:1783-1795(2011).
CC -!- FUNCTION: Transcription factor that may function in dorsoventral
CC specification of the forebrain. Regulates the expression of Wnt
CC signaling antagonists including the expression of a truncated TCF7L2
CC isoform that cannot bind CTNNB1 and acts therefore as a potent
CC dominant-negative Wnt antagonist. Plays a crucial role in eye
CC development and, in particular, in the specification of the ventral
CC optic vesicle. May be a regulator of axial polarization in the retina.
CC {ECO:0000269|PubMed:10421837, ECO:0000269|PubMed:10595508,
CC ECO:0000269|PubMed:11830578, ECO:0000269|PubMed:21856776}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the developing and mature retina.
CC {ECO:0000269|PubMed:10421837, ECO:0000269|PubMed:10595508}.
CC -!- DEVELOPMENTAL STAGE: At 9.5 dpc, expressed solely in the ventral halves
CC of the optic stalks and vesicles without proximo-distal restriction.
CC Expression in the optic stalk diminishes after 11.5 dpc and becomes
CC restricted to the inner layer of optic cup in its ventral half.
CC Expression persists in the ventral halves of all neural retina layers
CC (inner and outer nuclear layer) at 14.5 dpc and 16.5 dpc, and the
CC ganglion cell layer, inner nuclear layer and photoreceptor layer in the
CC adult. {ECO:0000269|PubMed:10421837, ECO:0000269|PubMed:10485894}.
CC -!- SIMILARITY: Belongs to the EMX homeobox family. {ECO:0000305}.
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DR EMBL; AB020498; BAA76867.1; -; Genomic_DNA.
DR EMBL; Y17792; CAB56169.1; -; mRNA.
DR EMBL; AF028715; AAF21632.1; -; mRNA.
DR CCDS; CCDS20282.1; -.
DR RefSeq; NP_036042.1; NM_011912.3.
DR AlphaFoldDB; Q9WTP9; -.
DR SMR; Q9WTP9; -.
DR BioGRID; 204905; 5.
DR IntAct; Q9WTP9; 5.
DR STRING; 10090.ENSMUSP00000035976; -.
DR iPTMnet; Q9WTP9; -.
DR PhosphoSitePlus; Q9WTP9; -.
DR MaxQB; Q9WTP9; -.
DR PaxDb; Q9WTP9; -.
DR PRIDE; Q9WTP9; -.
DR ProteomicsDB; 297810; -.
DR Antibodypedia; 31160; 122 antibodies from 24 providers.
DR DNASU; 24113; -.
DR Ensembl; ENSMUST00000037807; ENSMUSP00000035976; ENSMUSG00000034777.
DR GeneID; 24113; -.
DR KEGG; mmu:24113; -.
DR UCSC; uc009cob.1; mouse.
DR CTD; 25806; -.
DR MGI; MGI:1346018; Vax2.
DR VEuPathDB; HostDB:ENSMUSG00000034777; -.
DR eggNOG; KOG0843; Eukaryota.
DR GeneTree; ENSGT00940000161043; -.
DR HOGENOM; CLU_071850_1_0_1; -.
DR InParanoid; Q9WTP9; -.
DR OMA; SAFEPYN; -.
DR OrthoDB; 1094882at2759; -.
DR PhylomeDB; Q9WTP9; -.
DR TreeFam; TF319504; -.
DR BioGRID-ORCS; 24113; 2 hits in 76 CRISPR screens.
DR ChiTaRS; Vax2; mouse.
DR PRO; PR:Q9WTP9; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9WTP9; protein.
DR Bgee; ENSMUSG00000034777; Expressed in optic fissure and 15 other tissues.
DR ExpressionAtlas; Q9WTP9; baseline and differential.
DR Genevisible; Q9WTP9; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:MGI.
DR GO; GO:0005634; C:nucleus; IDA:MGI.
DR GO; GO:0031490; F:chromatin DNA binding; IDA:MGI.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0001162; F:RNA polymerase II intronic transcription regulatory region sequence-specific DNA binding; IDA:NTNU_SB.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR GO; GO:0007409; P:axonogenesis; IMP:MGI.
DR GO; GO:0043010; P:camera-type eye development; IGI:MGI.
DR GO; GO:0007417; P:central nervous system development; IBA:GO_Central.
DR GO; GO:0009950; P:dorsal/ventral axis specification; IMP:MGI.
DR GO; GO:0048048; P:embryonic eye morphogenesis; IDA:MGI.
DR GO; GO:0030900; P:forebrain development; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR GO; GO:0030182; P:neuron differentiation; IBA:GO_Central.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0060041; P:retina development in camera-type eye; IDA:MGI.
DR GO; GO:0007601; P:visual perception; IEA:InterPro.
DR GO; GO:0016055; P:Wnt signaling pathway; IEA:UniProtKB-KW.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR000047; HTH_motif.
DR InterPro; IPR033021; Vax2.
DR PANTHER; PTHR24339:SF34; PTHR24339:SF34; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR PRINTS; PR00031; HTHREPRESSR.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation; Wnt signaling pathway.
FT CHAIN 1..292
FT /note="Ventral anterior homeobox 2"
FT /id="PRO_0000049352"
FT DNA_BIND 102..161
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..74
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 207..242
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..39
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 43..67
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..235
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 292 AA; 31701 MW; C6A76B7BB799445D CRC64;
MGDGGAERDR GPKRREEPGG RSGRHGEHRG AEDLRADTGS ASPREIAGTS ASSPAGSRES
GGDSDGQQAL GETDHCRRIL VRDAKGTIRE IVLPKGLDLD RPKRTRTSFT AEQLYRLEME
FQRCQYVVGR ERTELARQLN LSETQVKVWF QNRRTKQKKD QSRDLEKRAS SSASEAFATS
NVLRLLEQGR LLSVPRAPSL LALTPGLPGL PASHRGTSLV DPRNSSPRLN PMPSASASSP
LPPPLPAICF SSAPLLDLPA GYKLGSSAFE PYSRLEQQKV GSPGQSDKKA DI
