VBA1_YEAST
ID VBA1_YEAST Reviewed; 562 AA.
AC Q04301; D6VZR1;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 1.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Vacuolar basic amino acid transporter 1;
GN Name=VBA1; OrderedLocusNames=YMR088C; ORFNames=YM9582.13C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15572352; DOI=10.1074/jbc.m412617200;
RA Shimazu M., Sekito T., Akiyama K., Ohsumi Y., Kakinuma Y.;
RT "A family of basic amino acid transporters of the vacuolar membrane from
RT Saccharomyces cerevisiae.";
RL J. Biol. Chem. 280:4851-4857(2005).
RN [4]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Transporter required for vacuolar uptake of at least
CC histidine and lysine. {ECO:0000269|PubMed:15572352}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:15572352};
CC Multi-pass membrane protein {ECO:0000269|PubMed:15572352}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; Z49259; CAA89235.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA09985.1; -; Genomic_DNA.
DR PIR; S54464; S54464.
DR RefSeq; NP_013806.1; NM_001182588.1.
DR AlphaFoldDB; Q04301; -.
DR BioGRID; 35263; 100.
DR DIP; DIP-888N; -.
DR STRING; 4932.YMR088C; -.
DR TCDB; 2.A.1.48.1; the major facilitator superfamily (mfs).
DR MaxQB; Q04301; -.
DR PaxDb; Q04301; -.
DR PRIDE; Q04301; -.
DR EnsemblFungi; YMR088C_mRNA; YMR088C; YMR088C.
DR GeneID; 855113; -.
DR KEGG; sce:YMR088C; -.
DR SGD; S000004694; VBA1.
DR VEuPathDB; FungiDB:YMR088C; -.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_000960_22_3_1; -.
DR InParanoid; Q04301; -.
DR OMA; FYLWRSI; -.
DR BioCyc; YEAST:G3O-32788-MON; -.
DR PRO; PR:Q04301; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; Q04301; protein.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015174; F:basic amino acid transmembrane transporter activity; IDA:SGD.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015802; P:basic amino acid transport; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..562
FT /note="Vacuolar basic amino acid transporter 1"
FT /id="PRO_0000173428"
FT TOPO_DOM 1..30
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 31..51
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 52..100
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 101..121
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 122..131
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 132..152
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 153..166
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..190
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 191..211
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 212..232
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 233..253
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 254..255
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 256..276
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 277..292
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 293..313
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 314..331
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 353..365
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 366..386
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 387..419
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 420..440
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 441..448
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 449..469
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 470..528
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 529..549
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 550..562
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 123
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 324
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 504
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 562 AA; 62640 MW; 08C6675482A5F3F2 CRC64;
MQTLDETSNL LPPPEEAEAP PLEQKFHEYN LALPKFPILF SLWLGSFLSS LDSTIVANIM
NRVAEEFSES SKKQWIATSF LLTNTAFQPL YGKLSDITGR KSALLTAQFF FGLGCLLTCF
ARNVTEFSIA RAICGIGAGG LNAISSIAVS DICTARERGV YQGYANIVFG FGQLLGAPLG
GVFIETIGWR ALFGIQVPVI MLCSVLAIKN INIKLFHVPP MKERYTLKNL SRIDIFGSLS
LVATISGVLF LCSSQLNKLY LALFTIGSFI VFILVERYYA TEKILPFELL TRSFCLSSAV
TVISSFVVFG EIFRSPIYLQ LLQNISVTKT GLFLIFPSIS VAVGSLVTGW VLRNTKINLA
HCAYQIIFGG MIMQLLGLGL GYFLLSHLNP DYTIYDMLES ITFRSNSIWW KLIYVFASVL
VSFGYACLLV ATLVSIVFTV EKSQQGTMTG VFYLWRSIGN VLGASLTLVS YENSLSSMLW
NYMFKTKRDD EYHFTKKQYY SLINDSSYLR GPNFPTDIFV RILDVYKKAF LISYIPNIAL
AAVGIVLSLY LVKHTYKRSS SS
