VBA2_YEAST
ID VBA2_YEAST Reviewed; 474 AA.
AC P38358; D6VQT7;
DT 01-OCT-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1994, sequence version 1.
DT 03-AUG-2022, entry version 159.
DE RecName: Full=Vacuolar basic amino acid transporter 2;
GN Name=VBA2; OrderedLocusNames=YBR293W; ORFNames=YBR2109;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=7813418; DOI=10.1002/j.1460-2075.1994.tb06923.x;
RA Feldmann H., Aigle M., Aljinovic G., Andre B., Baclet M.C., Barthe C.,
RA Baur A., Becam A.-M., Biteau N., Boles E., Brandt T., Brendel M.,
RA Brueckner M., Bussereau F., Christiansen C., Contreras R., Crouzet M.,
RA Cziepluch C., Demolis N., Delaveau T., Doignon F., Domdey H.,
RA Duesterhus S., Dubois E., Dujon B., El Bakkoury M., Entian K.-D.,
RA Feuermann M., Fiers W., Fobo G.M., Fritz C., Gassenhuber J., Glansdorff N.,
RA Goffeau A., Grivell L.A., de Haan M., Hein C., Herbert C.J.,
RA Hollenberg C.P., Holmstroem K., Jacq C., Jacquet M., Jauniaux J.-C.,
RA Jonniaux J.-L., Kallesoee T., Kiesau P., Kirchrath L., Koetter P.,
RA Korol S., Liebl S., Logghe M., Lohan A.J.E., Louis E.J., Li Z.Y.,
RA Maat M.J., Mallet L., Mannhaupt G., Messenguy F., Miosga T., Molemans F.,
RA Mueller S., Nasr F., Obermaier B., Perea J., Pierard A., Piravandi E.,
RA Pohl F.M., Pohl T.M., Potier S., Proft M., Purnelle B., Ramezani Rad M.,
RA Rieger M., Rose M., Schaaff-Gerstenschlaeger I., Scherens B.,
RA Schwarzlose C., Skala J., Slonimski P.P., Smits P.H.M., Souciet J.-L.,
RA Steensma H.Y., Stucka R., Urrestarazu L.A., van der Aart Q.J.M.,
RA Van Dyck L., Vassarotti A., Vetter I., Vierendeels F., Vissers S.,
RA Wagner G., de Wergifosse P., Wolfe K.H., Zagulski M., Zimmermann F.K.,
RA Mewes H.-W., Kleine K.;
RT "Complete DNA sequence of yeast chromosome II.";
RL EMBO J. 13:5795-5809(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP FUNCTION.
RX PubMed=15572352; DOI=10.1074/jbc.m412617200;
RA Shimazu M., Sekito T., Akiyama K., Ohsumi Y., Kakinuma Y.;
RT "A family of basic amino acid transporters of the vacuolar membrane from
RT Saccharomyces cerevisiae.";
RL J. Biol. Chem. 280:4851-4857(2005).
RN [5]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 208353 / W303-1A;
RX PubMed=16847258; DOI=10.1073/pnas.0604075103;
RA Kim H., Melen K., Oesterberg M., von Heijne G.;
RT "A global topology map of the Saccharomyces cerevisiae membrane proteome.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006).
CC -!- FUNCTION: Transporter required for vacuolar uptake of histidine,
CC arginine and lysine and to a lesser extent tyrosine.
CC {ECO:0000269|PubMed:15572352}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; Z36162; CAA85258.1; -; Genomic_DNA.
DR EMBL; AY692630; AAT92649.1; -; Genomic_DNA.
DR EMBL; BK006936; DAA07407.1; -; Genomic_DNA.
DR PIR; S46175; S46175.
DR RefSeq; NP_009852.3; NM_001178641.3.
DR AlphaFoldDB; P38358; -.
DR BioGRID; 32986; 53.
DR DIP; DIP-5142N; -.
DR IntAct; P38358; 4.
DR MINT; P38358; -.
DR STRING; 4932.YBR293W; -.
DR TCDB; 2.A.1.48.2; the major facilitator superfamily (mfs).
DR PaxDb; P38358; -.
DR PRIDE; P38358; -.
DR EnsemblFungi; YBR293W_mRNA; YBR293W; YBR293W.
DR GeneID; 852596; -.
DR KEGG; sce:YBR293W; -.
DR SGD; S000000497; VBA2.
DR VEuPathDB; FungiDB:YBR293W; -.
DR eggNOG; KOG0254; Eukaryota.
DR HOGENOM; CLU_000960_22_3_1; -.
DR InParanoid; P38358; -.
DR OMA; NWITTAY; -.
DR BioCyc; YEAST:G3O-29211-MON; -.
DR PRO; PR:P38358; -.
DR Proteomes; UP000002311; Chromosome II.
DR RNAct; P38358; protein.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IDA:SGD.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015174; F:basic amino acid transmembrane transporter activity; IDA:SGD.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015802; P:basic amino acid transport; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IDA:SGD.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..474
FT /note="Vacuolar basic amino acid transporter 2"
FT /id="PRO_0000173423"
FT TOPO_DOM 1..33
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..62
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 63..85
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 86..97
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..121
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 122..142
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 143..167
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..196
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..217
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 218..238
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..273
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 274..294
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 295..303
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 325..331
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 332..352
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 353..375
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 376..396
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 397..447
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 448..468
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 469..474
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 420
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 474 AA; 51677 MW; 1F3FDC877795ACFA CRC64;
MSISNWITTA YLITSTSFQP LYGSFSDALG RRNCLFFANG AFTIGCLACG FSKNIYMLSF
MRALTGIGGG GLITLSTIVN SDVIPSSKRG IFQAFQNLLL GFGAICGASF GGTIASSIGW
RWCFLIQVPI SVISSILMNY YVPNQKEYNR QNSSIFQNPG KILRDIDVMG SILIITGLTL
QLLYLSLGCS TSKLSWTSPS VLLLLVGSVI ILLLFILHER KTSARAIIPM ELVNSSYSVV
VLSISILVGF ASYAYLFTLP LFFQIVLGDS TAKAGLRLTI PSLFTPVGSL ITGFSMSKYN
CLRLLLYIGI SLMFLGNFLF LFIEKTSPNW LIGLFLIPAN LGQGITFPTT LFTFIFMFSK
SDQATATSTL YLFRSIGSVW GVAISAGVIQ LSFAGLLRSN LKGLLDENKI KKLIVQLSAN
SSYIGSLHGE VKNTVIKSFD EATKRAHLMS TLLSSLALIL CILKDNLAKP KTRR
