VBA5_YEAST
ID VBA5_YEAST Reviewed; 582 AA.
AC P36172; D6VXG6;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1994, sequence version 1.
DT 03-AUG-2022, entry version 154.
DE RecName: Full=Vacuolar basic amino acid transporter 5;
GN Name=VBA5; OrderedLocusNames=YKR105C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=8196765; DOI=10.1038/369371a0;
RA Dujon B., Alexandraki D., Andre B., Ansorge W., Baladron V.,
RA Ballesta J.P.G., Banrevi A., Bolle P.-A., Bolotin-Fukuhara M., Bossier P.,
RA Bou G., Boyer J., Buitrago M.J., Cheret G., Colleaux L.,
RA Daignan-Fornier B., del Rey F., Dion C., Domdey H., Duesterhoeft A.,
RA Duesterhus S., Entian K.-D., Erfle H., Esteban P.F., Feldmann H.,
RA Fernandes L., Fobo G.M., Fritz C., Fukuhara H., Gabel C., Gaillon L.,
RA Garcia-Cantalejo J.M., Garcia-Ramirez J.J., Gent M.E., Ghazvini M.,
RA Goffeau A., Gonzalez A., Grothues D., Guerreiro P., Hegemann J.H.,
RA Hewitt N., Hilger F., Hollenberg C.P., Horaitis O., Indge K.J.,
RA Jacquier A., James C.M., Jauniaux J.-C., Jimenez A., Keuchel H.,
RA Kirchrath L., Kleine K., Koetter P., Legrain P., Liebl S., Louis E.J.,
RA Maia e Silva A., Marck C., Monnier A.-L., Moestl D., Mueller S.,
RA Obermaier B., Oliver S.G., Pallier C., Pascolo S., Pfeiffer F.,
RA Philippsen P., Planta R.J., Pohl F.M., Pohl T.M., Poehlmann R.,
RA Portetelle D., Purnelle B., Puzos V., Ramezani Rad M., Rasmussen S.W.,
RA Remacha M.A., Revuelta J.L., Richard G.-F., Rieger M.,
RA Rodrigues-Pousada C., Rose M., Rupp T., Santos M.A., Schwager C.,
RA Sensen C., Skala J., Soares H., Sor F., Stegemann J., Tettelin H.,
RA Thierry A., Tzermia M., Urrestarazu L.A., van Dyck L.,
RA van Vliet-Reedijk J.C., Valens M., Vandenbol M., Vilela C., Vissers S.,
RA von Wettstein D., Voss H., Wiemann S., Xu G., Zimmermann J., Haasemann M.,
RA Becker I., Mewes H.-W.;
RT "Complete DNA sequence of yeast chromosome XI.";
RL Nature 369:371-378(1994).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP FUNCTION PREDICTION.
RX PubMed=15572352; DOI=10.1074/jbc.m412617200;
RA Shimazu M., Sekito T., Akiyama K., Ohsumi Y., Kakinuma Y.;
RT "A family of basic amino acid transporters of the vacuolar membrane from
RT Saccharomyces cerevisiae.";
RL J. Biol. Chem. 280:4851-4857(2005).
CC -!- FUNCTION: Transporter required for vacuolar uptake of basic amino
CC acids. {ECO:0000269|PubMed:15572352}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily.
CC {ECO:0000305}.
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DR EMBL; Z28330; CAA82185.1; -; Genomic_DNA.
DR EMBL; BK006944; DAA09256.1; -; Genomic_DNA.
DR PIR; S38184; S38184.
DR RefSeq; NP_013031.3; NM_001179895.3.
DR AlphaFoldDB; P36172; -.
DR BioGRID; 34236; 105.
DR DIP; DIP-5086N; -.
DR IntAct; P36172; 3.
DR MINT; P36172; -.
DR STRING; 4932.YKR105C; -.
DR PaxDb; P36172; -.
DR PRIDE; P36172; -.
DR EnsemblFungi; YKR105C_mRNA; YKR105C; YKR105C.
DR GeneID; 853980; -.
DR KEGG; sce:YKR105C; -.
DR SGD; S000001813; VBA5.
DR VEuPathDB; FungiDB:YKR105C; -.
DR eggNOG; KOG0254; Eukaryota.
DR GeneTree; ENSGT00940000176638; -.
DR HOGENOM; CLU_000960_22_1_1; -.
DR InParanoid; P36172; -.
DR OMA; GRWKVFP; -.
DR BioCyc; YEAST:G3O-32067-MON; -.
DR PRO; PR:P36172; -.
DR Proteomes; UP000002311; Chromosome XI.
DR RNAct; P36172; protein.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:1903826; P:L-arginine transmembrane transport; IMP:SGD.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 2.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 2.
DR PROSITE; PS50850; MFS; 1.
PE 3: Inferred from homology;
KW Amino-acid transport; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..582
FT /note="Vacuolar basic amino acid transporter 5"
FT /id="PRO_0000173427"
FT TOPO_DOM 1..44
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 45..65
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 66..80
FT /note="Vacuolar"
FT /evidence="ECO:0000250"
FT TRANSMEM 81..101
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 102..104
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..132
FT /note="Vacuolar"
FT /evidence="ECO:0000250"
FT TRANSMEM 133..153
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 154..160
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 182..191
FT /note="Vacuolar"
FT /evidence="ECO:0000250"
FT TRANSMEM 192..212
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 213..256
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 257..277
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 278..287
FT /note="Vacuolar"
FT /evidence="ECO:0000250"
FT TRANSMEM 288..308
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 309..329
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 330..350
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 351..372
FT /note="Vacuolar"
FT /evidence="ECO:0000250"
FT TRANSMEM 373..393
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 394..401
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 402..422
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 423..430
FT /note="Vacuolar"
FT /evidence="ECO:0000250"
FT TRANSMEM 431..451
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 452..469
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 470..492
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 493..539
FT /note="Vacuolar"
FT /evidence="ECO:0000250"
FT TRANSMEM 540..560
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 561..582
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT CARBOHYD 70
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 423
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 582 AA; 63361 MW; C89F6E2818B91D7E CRC64;
MEETKYSSQQ EIEGACGSDA SLNARGSNDS PMGLSLYLCL ASLTLVLFIT ALDILIVGTI
IDVVAEQFGN YSKTGWLVTG YSLPNAILSL IWGRFASIIG FQHSLILAIL IFEAGSLIAA
LASSMNMLIF GRVVAGVGGS GLQTLCFVIG CTMVGERSRP LVISILSCAF AVAAIVGPII
GGAFTTHVTW RWCFYINLPI GGLAIIMFLL TYKAENKGIL QQIKDAIGTI SSFTFSKFRH
QVNFKRLMNG IIFKFDFFGF ALCSAGLVLF LLGLTFGGNK YSWNSGQVIT YLVLGVLLFI
FSLVYDFFLF DKFNPEPDNI SYRPLLLRRL VAKPAIIIVN MVTFLLCTGY NGQMIYSVQF
FQLIFASSAW KAGLHLIPIV ITNVIAAIAS GVITKKLGLV KPLLIFGGVL GVIGAGLMTL
MTNTSTKSTQ IGVLLLPGFS LGFALQASLM SAQLQITKDR PEAAMDFIEV TAFNTFMKSL
GTTLGGVLST TVFSASFHNK VSRAHLEPYE GKTVDDMILY RLQNYDGSHS TIGNILSDSI
KNVFWMDLGF YALGFLFCSF SSNKKLIIPK KDDTPEDNLE DK
