VBF_ARATH
ID VBF_ARATH Reviewed; 282 AA.
AC Q9C7K0;
DT 23-JAN-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 102.
DE RecName: Full=F-box protein VBF;
DE AltName: Full=Protein PHLOEM PROTEIN 2-LIKE B14;
DE Short=AtPP2-B14;
DE AltName: Full=VIP1-binding F-box protein;
GN Name=VBF; Synonyms=PP2B14; OrderedLocusNames=At1g56250; ORFNames=F14G9.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=12529520; DOI=10.1104/pp.013086;
RA Dinant S., Clark A.M., Zhu Y., Vilaine F., Palauqui J.-C., Kusiak C.,
RA Thompson G.A.;
RT "Diversity of the superfamily of phloem lectins (phloem protein 2) in
RT angiosperms.";
RL Plant Physiol. 131:114-128(2003).
RN [6]
RP FUNCTION, INDUCTION BY AGROBACTERIUM, INTERACTION WITH VIP1; SKP1A AND
RP AGROBACTERIUM VIRE2, AND DISRUPTION PHENOTYPE.
RX PubMed=20227663; DOI=10.1016/j.chom.2010.02.009;
RA Zaltsman A., Krichevsky A., Loyter A., Citovsky V.;
RT "Agrobacterium induces expression of a host F-box protein required for
RT tumorigenicity.";
RL Cell Host Microbe 7:197-209(2010).
CC -!- FUNCTION: Component of SCF(VBF) E3 ubiquitin ligase complexes, which
CC mediate the ubiquitination and subsequent proteasomal degradation of
CC target proteins such as VIP1 and Agrobacterium virE2, after their
CC implication in T-DNA translocation to the host nucleus (can
CC functionally replace Agrobacterium VirF). Required during
CC Agrobacterium-induced tumor formation. {ECO:0000269|PubMed:20227663}.
CC -!- SUBUNIT: Component of SCF(VBF) E3 ubiquitin ligase complex that
CC interacts with VIP1. Interacts directly with SKP1A and VIP1. Forms a
CC complex composed of VIP1, VBF and Agrobacterium virE2.
CC {ECO:0000269|PubMed:20227663}.
CC -!- INDUCTION: By Agrobacterium. {ECO:0000269|PubMed:20227663}.
CC -!- DISRUPTION PHENOTYPE: Impaired Agrobacterium-mediated tumor formation.
CC {ECO:0000269|PubMed:20227663}.
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DR EMBL; AC069159; AAG50907.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE33369.1; -; Genomic_DNA.
DR EMBL; BT010431; AAQ62432.1; -; mRNA.
DR EMBL; AK175677; BAD43440.1; -; mRNA.
DR PIR; B96604; B96604.
DR RefSeq; NP_176021.1; NM_104504.3.
DR AlphaFoldDB; Q9C7K0; -.
DR BioGRID; 27303; 2.
DR IntAct; Q9C7K0; 2.
DR STRING; 3702.AT1G56250.1; -.
DR PaxDb; Q9C7K0; -.
DR PRIDE; Q9C7K0; -.
DR EnsemblPlants; AT1G56250.1; AT1G56250.1; AT1G56250.
DR GeneID; 842078; -.
DR Gramene; AT1G56250.1; AT1G56250.1; AT1G56250.
DR KEGG; ath:AT1G56250; -.
DR Araport; AT1G56250; -.
DR TAIR; locus:2011791; AT1G56250.
DR eggNOG; ENOG502QRA4; Eukaryota.
DR HOGENOM; CLU_050973_0_0_1; -.
DR InParanoid; Q9C7K0; -.
DR OMA; HWNISSK; -.
DR OrthoDB; 1051457at2759; -.
DR PhylomeDB; Q9C7K0; -.
DR PRO; PR:Q9C7K0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9C7K0; baseline and differential.
DR GO; GO:0030246; F:carbohydrate binding; ISS:TAIR.
DR InterPro; IPR036047; F-box-like_dom_sf.
DR InterPro; IPR001810; F-box_dom.
DR InterPro; IPR025886; PP2-like.
DR Pfam; PF14299; PP2; 1.
DR SUPFAM; SSF81383; SSF81383; 1.
DR PROSITE; PS50181; FBOX; 1.
PE 1: Evidence at protein level;
KW Crown gall tumor; Reference proteome; Ubl conjugation pathway.
FT CHAIN 1..282
FT /note="F-box protein VBF"
FT /id="PRO_0000272222"
FT DOMAIN 1..44
FT /note="F-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00080"
SQ SEQUENCE 282 AA; 31840 MW; E25B1DDE3A170B3E CRC64;
MMMLPEACIA NILAFTSPAD AFSSSEVSSV FRLAGDSDFV WEKFLPSDYK SLISQSTDHH
WNISSKKEIY RCLCDSLLID NARKLFKINK FSGKISYVLS ARDISITHSD HASYWSWSNV
SDSRFSESAE LIITDRLEIE GKIQTRVLSA NTRYGAYLIV KVTKGAYGLD LVPAETSIKS
KNGQISKSAT YLCCLDEKKQ QMKRLFYGNR EERMAMTVEA VGGDGKRREP KCRDDGWMEI
ELGEFETREG EDDEVNMTLT EVKGYQLKGG ILIDGIEVRP KT
