VCL6_JUGRE
ID VCL6_JUGRE Reviewed; 502 AA.
AC A0A2I4E5L6;
DT 07-OCT-2020, integrated into UniProtKB/Swiss-Prot.
DT 28-FEB-2018, sequence version 1.
DT 25-MAY-2022, entry version 16.
DE RecName: Full=Vicilin Jug r 6.0101 {ECO:0000305};
DE AltName: Full=Allergen Jug r 6 {ECO:0000303|PubMed:30054513};
DE AltName: Full=Vicilin Jug r 6 {ECO:0000303|PubMed:30054513};
DE AltName: Allergen=Jug r 6.0101 {ECO:0000305};
DE Flags: Precursor;
GN Name=LOC108986502 {ECO:0000312|RefSeq:XP_018814692.1};
OS Juglans regia (English walnut).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fagales; Juglandaceae; Juglans.
OX NCBI_TaxID=51240 {ECO:0000312|Proteomes:UP000235220};
RN [1] {ECO:0000312|Proteomes:UP000235220}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chandler {ECO:0000312|Proteomes:UP000235220};
RX PubMed=27145194; DOI=10.1111/tpj.13207;
RA Martinez-Garcia P.J., Crepeau M.W., Puiu D., Gonzalez-Ibeas D., Whalen J.,
RA Stevens K.A., Paul R., Butterfield T.S., Britton M.T., Reagan R.L.,
RA Chakraborty S., Walawage S.L., Vasquez-Gross H.A., Cardeno C., Famula R.A.,
RA Pratt K., Kuruganti S., Aradhya M.K., Leslie C.A., Dandekar A.M.,
RA Salzberg S.L., Wegrzyn J.L., Langley C.H., Neale D.B.;
RT "The walnut (Juglans regia) genome sequence reveals diversity in genes
RT coding for the biosynthesis of non-structural polyphenols.";
RL Plant J. 87:507-532(2016).
RN [2]
RP PROTEIN SEQUENCE OF 28-37; 45-65; 74-80; 81-124; 131-160; 161-210; 211-240;
RP 241-260; 261-290; 291-320; 321-343; 344-354; 358-384; 394-400; 401-480 AND
RP 481-502, BIOPHYSICOCHEMICAL PROPERTIES, SUBUNIT, TISSUE SPECIFICITY, PTM,
RP IDENTIFICATION BY MASS SPECTROMETRY, MASS SPECTROMETRY, ALLERGEN,
RP BIOTECHNOLOGY, PROTEOLYTIC CLEAVAGE, GLYCOSYLATION AT ASN-340, AND CIRCULAR
RP DICHROISM ANALYSIS.
RX PubMed=30054513; DOI=10.1038/s41598-018-29656-4;
RA Dubiela P., Kabasser S., Smargiasso N., Geiselhart S., Bublin M.,
RA Hafner C., Mazzucchelli G., Hoffmann-Sommergruber K.;
RT "Jug r 6 is the allergenic vicilin present in walnut responsible for IgE
RT cross-reactivities to other tree nuts and seeds.";
RL Sci. Rep. 8:11366-11366(2018).
CC -!- FUNCTION: Seed storage protein. {ECO:0000305|PubMed:30054513}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Temperature dependence:
CC Denaturation starts at 45 degrees Celsius. At temperatures above 75
CC degrees Celsius, begins to precipitate, and eventually becomes
CC irreversibly denatured. Cannot be resolubilized in aqueous solution
CC after being heated up to 95 degrees Celsius and then cooled down to
CC 25 degrees Celsius. {ECO:0000269|PubMed:30054513};
CC -!- SUBUNIT: Homotrimer. {ECO:0000269|PubMed:30054513}.
CC -!- TISSUE SPECIFICITY: Expressed in seed (at protein level).
CC {ECO:0000269|PubMed:30054513}.
CC -!- PTM: N-glycosylated; paucimannose-type structures containing xylose.
CC {ECO:0000269|PubMed:30054513}.
CC -!- MASS SPECTROMETRY: Mass=47155; Method=MALDI; Note=Non-glycosylated.;
CC Evidence={ECO:0000269|PubMed:30054513};
CC -!- MASS SPECTROMETRY: Mass=48829; Method=Electrospray; Note=Glycosylated.;
CC Evidence={ECO:0000269|PubMed:30054513};
CC -!- ALLERGEN: Causes an allergic reaction in human. Natural protein binds
CC to IgE in 26% of the 77 walnut-allergic patients tested. IgE-binding is
CC reduced by simulated gastric fluid and in vitro duodenal digestion, but
CC not by heat treatment. Cross-reacts with its counterparts from hazelnut
CC (Cor a 11), pistachio and sesame. {ECO:0000269|PubMed:30054513}.
CC -!- BIOTECHNOLOGY: Could be used as a marker of IgE cross-reactivity among
CC the vicilins of tree nuts in component resolved diagnosis.
CC {ECO:0000305|PubMed:30054513}.
CC -!- SIMILARITY: Belongs to the 7S seed storage protein family.
CC {ECO:0000305}.
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DR RefSeq; XP_018814692.1; XM_018959147.1.
DR AlphaFoldDB; A0A2I4E5L6; -.
DR SMR; A0A2I4E5L6; -.
DR STRING; 51240.A0A2I4E5L6; -.
DR Allergome; 12005; Jug r 6.
DR Allergome; 12006; Jug r 6.0101.
DR EnsemblPlants; Jr08_13930_p1; cds.Jr08_13930_p1; Jr08_13930.
DR GeneID; 108986502; -.
DR Gramene; Jr08_13930_p1; cds.Jr08_13930_p1; Jr08_13930.
DR KEGG; jre:108986502; -.
DR OrthoDB; 1072107at2759; -.
DR Proteomes; UP000235220; Chromosome 8.
DR GO; GO:0043245; C:extraorganismal space; IDA:UniProtKB.
DR GO; GO:0045735; F:nutrient reservoir activity; IC:UniProtKB.
DR GO; GO:0070207; P:protein homotrimerization; IDA:UniProtKB.
DR GO; GO:0010431; P:seed maturation; IEP:UniProtKB.
DR Gene3D; 2.60.120.10; -; 2.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 2.
DR SMART; SM00835; Cupin_1; 2.
DR SUPFAM; SSF51182; SSF51182; 2.
PE 1: Evidence at protein level;
KW Allergen; Direct protein sequencing; Glycoprotein; Reference proteome;
KW Seed storage protein; Signal; Storage protein.
FT SIGNAL 1..27
FT /evidence="ECO:0000269|PubMed:30054513"
FT CHAIN 28..502
FT /note="Vicilin Jug r 6.0101"
FT /evidence="ECO:0000305|PubMed:30054513"
FT /id="PRO_5014181227"
FT DOMAIN 101..259
FT /note="Cupin type-1 1"
FT /evidence="ECO:0000255"
FT DOMAIN 302..475
FT /note="Cupin type-1 2"
FT /evidence="ECO:0000255"
FT REGION 67..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 374..401
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 67..88
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 73..74
FT /note="Cleavage"
FT /evidence="ECO:0000269|PubMed:30054513"
FT CARBOHYD 340
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:30054513"
SQ SEQUENCE 502 AA; 57400 MW; 2736C0C0334522B5 CRC64;
MAFKPKIPIA LLLLTSLLAI CAGLALAMQD PELKQCKHQC RHQRQFDEQE KEHCQRSCDE
YHIEKKARER AERRRSEEGS SREEGYEEEE LGGEREEENP YVFEDEDFET RVRTDEGRIQ
VLEKFTKRSK LLRGIENFRV AILEANPQTF ISPAHFDAEL VVFVAKGRAT ITTVREEKRE
NFNVEQGDIM RIPAGTPVYL INRDENEKLY IVKILRPVSV PGHFEAFHGS GGEDPESFYR
AFSWEVLEAA LKTRRDQLEK LFGKQTQGVI IKASKEQIRS MSKHEETTPR IWPFGGDSTH
PFNLFHKRPS QSNQFGRLFE TDPKECKQLQ DLDLMVSFAN ITKGSMAGPY YNSRATKISV
VIEGEGYFEM ACPHLSSSGS RGQREGSGSS RRRSRSGPSY QQIRGRLRPG MVFVAPAGHP
VAVIASRNKN LQVLCFDVNA QGNIRFPLAG KNNIVNEFEK EAKELAFNFP AREVEKIFRN
QDQEFFFPGP SRQPEEGGRA FE
