ID   VCLB_PEA                Reviewed;         410 AA.
AC   P02854;
DT   21-JUL-1986, integrated into UniProtKB/Swiss-Prot.
DT   21-JUL-1986, sequence version 1.
DT   25-MAY-2022, entry version 93.
DE   RecName: Full=Provicilin;
DE   AltName: Full=Type B;
DE   Flags: Precursor; Fragment;
OS   Pisum sativum (Garden pea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX   NCBI_TaxID=3888;
RN   [1]
RP   NUCLEOTIDE SEQUENCE (CLONES PDUB7 AND PDUB4), AND GLYCOSYLATION AT ASN-359.
RC   STRAIN=cv. Feltham First;
RX   PubMed=6687941; DOI=10.1093/nar/11.8.2367;
RA   Lycett G.W., Delauney A.J., Gatehouse J.A., Gilroy J., Croy R.R.D.,
RA   Boulter D.;
RT   "The vicilin gene family of pea (Pisum sativum L.): a complete cDNA coding
RT   sequence for preprovicilin.";
RL   Nucleic Acids Res. 11:2367-2380(1983).
CC   -!- FUNCTION: Seed storage protein.
CC   -!- SUBCELLULAR LOCATION: Vacuole, aleurone grain. Vacuole.
CC       Note=Cotyledonary membrane-bound vacuolar protein bodies.
CC   -!- SIMILARITY: Belongs to the 7S seed storage protein family.
CC       {ECO:0000305}.
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DR   PIR; A03344; FWPMVB.
DR   AlphaFoldDB; P02854; -.
DR   SMR; P02854; -.
DR   Allergome; 947; Pis s 1.
DR   iPTMnet; P02854; -.
DR   GO; GO:0033095; C:aleurone grain; IEA:UniProtKB-SubCell.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.10; -; 2.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 2.
DR   SMART; SM00835; Cupin_1; 2.
DR   SUPFAM; SSF51182; SSF51182; 2.
PE   1: Evidence at protein level;
KW   Glycoprotein; Seed storage protein; Signal; Storage protein; Vacuole.
FT   SIGNAL          1..15
FT   CHAIN           16..>410
FT                   /note="Provicilin"
FT                   /id="PRO_0000032182"
FT   DOMAIN          23..181
FT                   /note="Cupin type-1 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          241..409
FT                   /note="Cupin type-1 2"
FT                   /evidence="ECO:0000255"
FT   REGION          223..242
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          312..331
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        228..242
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        313..331
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   SITE            221..222
FT                   /note="Cleavage"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        359
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000269|PubMed:6687941"
FT   NON_TER         410
SQ   SEQUENCE   410 AA;  46385 MW;  8AF68CE85A316FA2 CRC64;
     MLLAIAFLAS VCVSSRSDQE NPFIFKSNRF QTLYENENGH IRLLQKFDKR SKIFENLQNY
     RLLEYKSKPH TLFLPQYTDA DFILVVLSGK ATLTVLKSND RNSFNLERGD AIKLPAGSIA
     YFANRDDNEE PRVLDLAIPV NKPGQLQSFL LSGTQNQKSS LSGFSKNILE AAFNTNYEEI
     EKVLLEQQEQ EPQHRRSLKD RRQEINEENV IVKVSRDQIE ELSKNAKSSS KKSVSSESGP
     FNLRSRNPIY SNKFGKFFEI TPEKNQQLQD LDIFVNSVDI KVGSLLLPNY NSRAIVIVTV
     TEGKGDFELV GQRNENQGKE NDKEEEQEEE TSKQVQLYRA KLSPGDVFVI PAGHPVAINA
     SSDLNLIGLG INAENNERNF LAGEEDNVIS QVERPVKELA FPGSSHEVDR