VCLC_PEA
ID VCLC_PEA Reviewed; 459 AA.
AC P13918;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-APR-1990, sequence version 2.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Vicilin;
DE Flags: Precursor;
OS Pisum sativum (Garden pea).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; Hologalegina; IRL clade; Fabeae; Pisum.
OX NCBI_TaxID=3888;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC TISSUE=Seedling;
RX AGRICOLA=IND92000013; DOI=10.1007/BF00017468;
RA Higgins T.J.V., Newbigin E.J., Spencer D., Llewellyn D.J., Craig S.;
RT "The sequence of a pea vicilin gene and its expression in transgenic
RT tobacco.";
RL Plant Mol. Biol. 11:683-695(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 27-459 (CLONE PDUB9).
RC STRAIN=cv. Feltham First;
RX PubMed=3046604; DOI=10.1042/bj2510857;
RA Watson M.D., Lambert N., Delauney A., Yarwood J.N., Croy R.R.D.,
RA Gatehouse J.A., Wright D.J., Boulter D.;
RT "Isolation and expression of a pea vicilin cDNA in the yeast Saccharomyces
RT cerevisiae.";
RL Biochem. J. 251:857-864(1988).
CC -!- FUNCTION: Seed storage protein.
CC -!- SUBCELLULAR LOCATION: Vacuole, aleurone grain. Vacuole.
CC Note=Cotyledonary membrane-bound vacuolar protein bodies.
CC -!- MISCELLANEOUS: There are at least 11 genes for vicilin subunits.
CC -!- SIMILARITY: Belongs to the 7S seed storage protein family.
CC {ECO:0000305}.
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DR EMBL; X14076; CAA32239.1; -; Genomic_DNA.
DR EMBL; Y00722; CAA68708.1; -; mRNA.
DR PIR; S00567; S00567.
DR PIR; S08505; S08505.
DR AlphaFoldDB; P13918; -.
DR SMR; P13918; -.
DR Allergome; 947; Pis s 1.
DR PRIDE; P13918; -.
DR GO; GO:0033095; C:aleurone grain; IEA:UniProtKB-SubCell.
DR GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR Gene3D; 2.60.120.10; -; 2.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 2.
DR SMART; SM00835; Cupin_1; 2.
DR SUPFAM; SSF51182; SSF51182; 2.
PE 2: Evidence at transcript level;
KW Seed storage protein; Signal; Storage protein; Vacuole.
FT SIGNAL 1..28
FT CHAIN 29..459
FT /note="Vicilin"
FT /id="PRO_0000032183"
FT DOMAIN 36..194
FT /note="Cupin type-1 1"
FT /evidence="ECO:0000255"
FT DOMAIN 254..426
FT /note="Cupin type-1 2"
FT /evidence="ECO:0000255"
FT REGION 235..258
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 321..346
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 430..459
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..255
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 443..459
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 375
FT /note="V -> L (in Ref. 2; CAA68708)"
FT /evidence="ECO:0000305"
FT CONFLICT 409
FT /note="I -> V (in Ref. 2; CAA68708)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 459 AA; 52231 MW; E2FA390B95451D29 CRC64;
MAATTMKASF PLLMLMGISF LASVCVSSRS DPQNPFIFKS NKFQTLFENE NGHIRLLQKF
DQRSKIFENL QNYRLLEYKS KPHTIFLPQH TDADYILVVL SGKAILTVLK PDDRNSFNLE
RGDTIKLPAG TIAYLVNRDD NEELRVLDLA IPVNRPGQLQ SFLLSGNQNQ QNYLSGFSKN
ILEASFNTDY EEIEKVLLEE HEKETQHRRS LKDKRQQSQE ENVIVKLSRG QIEELSKNAK
STSKKSVSSE SEPFNLRSRG PIYSNEFGKF FEITPEKNPQ LQDLDIFVNS VEIKEGSLLL
PHYNSRAIVI VTVNEGKGDF ELVGQRNENQ QEQRKEDDEE EEQGEEEINK QVQNYKAKLS
SGDVFVIPAG HPVAVKASSN LDLLGFGINA ENNQRNFLAG DEDNVISQIQ RPVKELAFPG
SAQEVDRILE NQKQSHFADA QPQQRERGSR ETRDRLSSV
