VCL_CORAV
ID VCL_CORAV Reviewed; 448 AA.
AC Q8S4P9;
DT 10-FEB-2021, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 56.
DE RecName: Full=Vicilin Cor a 11.0101 {ECO:0000305};
DE AltName: Full=7S globulin {ECO:0000303|PubMed:19006093, ECO:0000303|PubMed:21563837, ECO:0000303|PubMed:21735061, ECO:0000303|PubMed:31753489};
DE AltName: Full=7S seed storage protein {ECO:0000303|PubMed:19006093};
DE AltName: Full=7S vicilin-like protein Cor a 11 {ECO:0000303|PubMed:22812192, ECO:0000303|Ref.12};
DE AltName: Full=Allergen Cor a 11 {ECO:0000303|PubMed:21563837, ECO:0000303|PubMed:22966848, ECO:0000303|PubMed:23411333, ECO:0000303|Ref.12};
DE AltName: Full=Vicilin Cor a 11 {ECO:0000303|PubMed:15233621, ECO:0000303|PubMed:21563837, ECO:0000303|PubMed:21735061, ECO:0000303|PubMed:22616776};
DE AltName: Full=Vicilin HZ.1 {ECO:0000303|PubMed:31753489};
DE AltName: Allergen=Cor a 11.0101 {ECO:0000305};
OS Corylus avellana (European hazel) (Corylus maxima).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fagales; Betulaceae; Corylus.
OX NCBI_TaxID=13451 {ECO:0000312|EMBL:AAL86739.1};
RN [1] {ECO:0000312|EMBL:AAL86739.1}
RP NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 48-59; 56-72; 73-79; 80-89;
RP 92-100; 101-127; 130-136; 140-152; 153-174; 175-201; 202-213; 241-251;
RP 252-268; 269-278; 279-304; 305-315; 319-349; 353-388; 389-394; 395-403;
RP 407-415; 416-419; 420-442 AND 423-445, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, PTM, IDENTIFICATION BY MASS SPECTROMETRY, ALLERGEN, SITE,
RP GLYCOSYLATION AT ASN-301, AND CIRCULAR DICHROISM ANALYSIS.
RC STRAIN=cv. Piemonte {ECO:0000312|EMBL:AAL86739.1};
RC TISSUE=Seed {ECO:0000303|PubMed:15233621};
RX PubMed=15233621; DOI=10.1042/bj20041062;
RA Lauer I., Foetisch K., Kolarich D., Ballmer-Weber B.K., Conti A.,
RA Altmann F., Vieths S., Scheurer S.;
RT "Hazelnut (Corylus avellana) vicilin Cor a 11: molecular characterization
RT of a glycoprotein and its allergenic activity.";
RL Biochem. J. 383:327-334(2004).
RN [2]
RP PROTEIN SEQUENCE OF 1-11; 5-13; 73-89; 141-152; 175-201; 216-228; 305-315
RP AND 395-403, TISSUE SPECIFICITY, IDENTIFICATION BY MASS SPECTROMETRY, AND
RP BIOTECHNOLOGY.
RX PubMed=25209075; DOI=10.1021/jf504007d;
RA De Ceglie C., Calvano C.D., Zambonin C.G.;
RT "Determination of hidden hazelnut oil proteins in extra virgin olive oil by
RT cold acetone precipitation followed by in-solution tryptic digestion and
RT MALDI-TOF-MS analysis.";
RL J. Agric. Food Chem. 62:9401-9409(2014).
RN [3]
RP PROTEIN SEQUENCE OF 48-62; 73-89; 92-127; 130-136; 140-169; 175-213;
RP 241-278; 305-315; 319-345; 353-388; 395-403; 407-415 AND 423-442, SUBUNIT,
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, PTM, IDENTIFICATION BY MASS
RP SPECTROMETRY, ALLERGEN, AND CIRCULAR DICHROISM ANALYSIS.
RC TISSUE=Seed {ECO:0000303|PubMed:19006093};
RX PubMed=19006093; DOI=10.1002/mnfr.200800083;
RA Rigby N.M., Marsh J., Sancho A.I., Wellner K., Akkerdaas J., van Ree R.,
RA Knulst A., Fernandez-Rivas M., Brettlova V., Schilte P.P., Summer C.,
RA Pumphrey R., Shewry P.R., Mills E.N.;
RT "The purification and characterisation of allergenic hazelnut seed
RT proteins.";
RL Mol. Nutr. Food Res. 52:S251-S261(2008).
RN [4] {ECO:0007744|PDB:6L4C}
RP PROTEIN SEQUENCE OF 48-67, X-RAY CRYSTALLOGRAPHY (3.19 ANGSTROMS) OF 25-448
RP IN COMPLEX WITH COPPER, FUNCTION, SUBUNIT, TISSUE SPECIFICITY, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=31753489; DOI=10.1016/j.bbrc.2019.11.072;
RA Shikhi M., Jain A., Salunke D.M.;
RT "Comparative study of 7S globulin from Corylus avellana and Solanum
RT lycopersicum revealed importance of salicylic acid and Cu-binding loop in
RT modulating their function.";
RL Biochem. Biophys. Res. Commun. 522:127-132(2020).
RN [5]
RP PROTEIN SEQUENCE OF 56-72; 80-89; 92-100; 101-127; 141-152; 153-169;
RP 175-201; 202-213; 241-251; 252-268; 269-278; 279-288; 305-315; 395-403;
RP 407-415 AND 423-435, TISSUE SPECIFICITY, IDENTIFICATION BY MASS
RP SPECTROMETRY, AND BIOTECHNOLOGY.
RX PubMed=22966848; DOI=10.1080/19440049.2012.719639;
RA Cucu T., De Meulenaer B., Devreese B.;
RT "MALDI-based identification of stable hazelnut protein derived tryptic
RT marker peptides.";
RL Food Addit. Contam. Part A Chem. Anal. Control Expo. Risk Assess.
RL 29:1821-1831(2012).
RN [6]
RP PROTEIN SEQUENCE OF 92-100; 202-213 AND 407-415, TISSUE SPECIFICITY,
RP IDENTIFICATION BY MASS SPECTROMETRY, AND BIOTECHNOLOGY.
RX PubMed=21735061; DOI=10.1007/s00216-011-5218-6;
RA Ansari P., Stoppacher N., Baumgartner S.;
RT "Marker peptide selection for the determination of hazelnut by LC-MS/MS and
RT occurrence in other nuts.";
RL Anal. Bioanal. Chem. 402:2607-2615(2012).
RN [7]
RP PROTEIN SEQUENCE OF 92-100; 202-213 AND 407-415, TISSUE SPECIFICITY,
RP IDENTIFICATION BY MASS SPECTROMETRY, AND BIOTECHNOLOGY.
RX PubMed=24577577; DOI=10.1007/s00216-014-7679-x;
RA Costa J., Ansari P., Mafra I., Oliveira M.B., Baumgartner S.;
RT "Assessing hazelnut allergens by protein- and DNA-based approaches: LC-
RT MS/MS, ELISA and real-time PCR.";
RL Anal. Bioanal. Chem. 406:2581-2590(2014).
RN [8]
RP TISSUE SPECIFICITY, AND ALLERGEN.
RX PubMed=21563837; DOI=10.1021/jf2007375;
RA Iwan M., Vissers Y.M., Fiedorowicz E., Kostyra H., Kostyra E.,
RA Savelkoul H.F., Wichers H.J.;
RT "Impact of Maillard reaction on immunoreactivity and allergenicity of the
RT hazelnut allergen Cor a 11.";
RL J. Agric. Food Chem. 59:7163-7171(2011).
RN [9]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, ALLERGEN, AND 3D-STRUCTURE
RP MODELING.
RX PubMed=22616776; DOI=10.1186/2043-9113-2-12;
RA Lopez E., Cuadrado C., Burbano C., Jimenez M.A., Rodriguez J., Crespo J.F.;
RT "Effects of autoclaving and high pressure on allergenicity of hazelnut
RT proteins.";
RL J. Clin. Bioinforma. 2:12-12(2012).
RN [10]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND ALLERGEN.
RX PubMed=22812192;
RA Verweij M.M., Hagendorens M.M., Trashin S., Cucu T., De Meulenaer B.,
RA Devreese B., Bridts C.H., De Clerck L.S., Ebo D.G.;
RT "Age-dependent sensitization to the 7S-vicilin-like protein Cor a 11 from
RT hazelnut (Corylus avellana) in a birch-endemic region.";
RL J. Investig. Allergol. Clin. Immunol. 22:245-251(2012).
RN [11]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND BIOTECHNOLOGY.
RX PubMed=23411333; DOI=10.1016/j.foodchem.2012.11.036;
RA Iniesto E., Jimenez A., Prieto N., Cabanillas B., Burbano C., Pedrosa M.M.,
RA Rodriguez J., Muzquiz M., Crespo J.F., Cuadrado C., Linacero R.;
RT "Real Time PCR to detect hazelnut allergen coding sequences in processed
RT foods.";
RL Food Chem. 138:1976-1981(2013).
RN [12]
RP TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND BIOTECHNOLOGY.
RX DOI=10.1111/ijfs.12079;
RA Garino C., Locatelli M., Coisson J.D., D'Andrea M., Cereti E.,
RA Travaglia F., Arlorio M.;
RT "Gene transcription analysis of hazelnut (C orylus avellana L.) allergens
RT Cor a 1, Cor a 8 and Cor a 11: a comparative study.";
RL Int. J. Food Sci. Technol. 48:1208-1217(2013).
RN [13]
RP ALLERGEN.
RX PubMed=34146442; DOI=10.1111/pai.13579;
RA Nebbia S., Lamberti C., Cirrincione S., Acquadro A., Abba S., Ciuffo M.,
RA Torello Marinoni D., Manfredi M., Marengo E., Calzedda R., Monti G.,
RA Cavallarin L., Giuffrida M.G.;
RT "Oleosin Cor a 15 is a novel allergen for Italian hazelnut allergic
RT children.";
RL Pediatr. Allergy Immunol. 32:1743-1755(2021).
CC -!- FUNCTION: Seed storage protein (PubMed:15233621, PubMed:19006093,
CC PubMed:21563837, PubMed:21735061, PubMed:22966848, PubMed:22616776,
CC PubMed:22812192, PubMed:23411333, Ref.12, PubMed:24577577,
CC PubMed:25209075). Does not have superoxide dismutase (SOD) activity
CC (PubMed:31753489). {ECO:0000269|PubMed:31753489,
CC ECO:0000305|PubMed:15233621, ECO:0000305|PubMed:19006093,
CC ECO:0000305|PubMed:21563837, ECO:0000305|PubMed:21735061,
CC ECO:0000305|PubMed:22616776, ECO:0000305|PubMed:22812192,
CC ECO:0000305|PubMed:22966848, ECO:0000305|PubMed:23411333,
CC ECO:0000305|PubMed:24577577, ECO:0000305|PubMed:25209075,
CC ECO:0000305|Ref.12}.
CC -!- SUBUNIT: Homotrimer (PubMed:19006093, PubMed:31753489). Homohexamer
CC (PubMed:19006093). {ECO:0000269|PubMed:19006093,
CC ECO:0000269|PubMed:31753489}.
CC -!- TISSUE SPECIFICITY: Expressed in seed (at protein level)
CC (PubMed:15233621, PubMed:19006093, PubMed:21563837, PubMed:21735061,
CC PubMed:22966848, PubMed:22616776, PubMed:22812192, PubMed:23411333,
CC PubMed:24577577, PubMed:25209075, PubMed:31753489). Expressed in seed
CC (Ref.12). {ECO:0000269|PubMed:15233621, ECO:0000269|PubMed:19006093,
CC ECO:0000269|PubMed:21563837, ECO:0000269|PubMed:21735061,
CC ECO:0000269|PubMed:22616776, ECO:0000269|PubMed:22812192,
CC ECO:0000269|PubMed:22966848, ECO:0000269|PubMed:23411333,
CC ECO:0000269|PubMed:24577577, ECO:0000269|PubMed:25209075,
CC ECO:0000269|PubMed:31753489, ECO:0000269|Ref.12}.
CC -!- DEVELOPMENTAL STAGE: Expressed during seed maturation. Expressed at
CC three fruit developmental stages, at early stage (approximately 45 days
CC before harvest), at middle stage (approximately 30 days before harvest)
CC and at final harvest stage. Expressed more in ripe than in unripe seeds
CC (Ref.12). Expressed in raw seeds (PubMed:15233621, PubMed:19006093,
CC PubMed:22616776, PubMed:22812192, PubMed:23411333).
CC {ECO:0000269|PubMed:15233621, ECO:0000269|PubMed:19006093,
CC ECO:0000269|PubMed:22616776, ECO:0000269|PubMed:22812192,
CC ECO:0000269|PubMed:23411333, ECO:0000269|Ref.12}.
CC -!- PTM: N-glycosylated at Asn-301 mostly with xylosylated paucimannosidic-
CC type N-glycan MMX (an N-linked glycan with beta-1,2-xylose residue in
CC the structure) and also with MMXF (a complex N-linked glycan with
CC alpha-1,3-fucose and beta-1,2-xylose residues in the structure).
CC {ECO:0000269|PubMed:15233621}.
CC -!- PTM: A mixture of proteolytically processed and unprocessed subunits
CC exist. {ECO:0000269|PubMed:19006093}.
CC -!- ALLERGEN: Causes an allergic reaction in human (PubMed:15233621,
CC PubMed:19006093, PubMed:21563837, PubMed:22616776, PubMed:22812192,
CC PubMed:34146442). Binds to IgE of patients allergic to hazelnuts
CC (PubMed:15233621, PubMed:19006093, PubMed:21563837, PubMed:22616776,
CC PubMed:22812192, PubMed:34146442). Natural glycosylated protein binds
CC to IgE in 47% and recombinant (non-glycosylated) protein in 43-40% of
CC the 65 tested adult patients from Switzerland and Germany
CC (PubMed:15233621). Natural protein binds to IgE in 50% of the tested
CC patients from Netherlands (PubMed:19006093). The IgE-binding of the
CC natural protein can be decreased by glycation (Maillard reaction) of
CC the protein at 60 and 145 (routine hazelnut roasting condition), but
CC not at 37 degrees Celsius (PubMed:21563837). IgE-binding of the natural
CC protein is also strongly reduced by autoclaving at 138 degrees Celsius
CC for 15 or 30 minutes, but not by high pressure treatment alone (300
CC Mba, 400 Mba, 500 Mba and 600 Mba) (PubMed:22616776). Allerginicity to
CC this protein in a birch-endemic region (Belgium) seems to be
CC predominantly found in children with severe hazelnut allergy compared
CC to adults or children with milder forms of hazelnut-allergy. Natural
CC protein binds to IgE in 36%, 40% and 12.5% of the 22 preschool
CC children, 10 schoolchildren and 8 adults tested, respectively, with
CC systemic allergic reactions toward hazelnut. In a set of 40 patients (6
CC preschool children, 10 schoolchildren and 24 adults) having oral
CC allergy symptoms, only 2 patients (of preschool age) show IgE-binding
CC to this protein. Also, only 8% of the 24 hazelnut-allergic infants with
CC atopic dermatitis tested show IgE reactivity to this protein
CC (PubMed:22812192). Induces histamine release from human basophils
CC (PubMed:15233621, PubMed:22812192). Both natural and recombinant
CC proteins induce histamine release from human basophils in a similar
CC manner indicating that the carbohydrate structures are not involved in
CC IgE-binding (PubMed:15233621). Induces beta-hexosaminidase release from
CC humanized rat basophilic leukemia (RBL) cells. Heating, with or without
CC glucose, at 145 degrees Celsius increases the basophil degranulation
CC capacity (PubMed:21563837). {ECO:0000269|PubMed:15233621,
CC ECO:0000269|PubMed:19006093, ECO:0000269|PubMed:21563837,
CC ECO:0000269|PubMed:22616776, ECO:0000269|PubMed:22812192,
CC ECO:0000269|PubMed:34146442}.
CC -!- BIOTECHNOLOGY: In order to protect patients that are allergic to
CC hazelnuts, it is extremely important to find ways to detect traces
CC amounts of hazelnut in foods that should not contain it for the food
CC labeling and safety purposes. This protein is used in the development
CC of these methods (PubMed:21735061, PubMed:22966848, PubMed:23411333,
CC PubMed:24577577, PubMed:25209075). For the detection of hazelnut, a
CC liquid chromatography tandem mass spectrometry (LC-MS/MS) method in
CC selected reaction monitoring (SRM) mode is developed by using selected
CC marker peptides from this protein as standards (PubMed:21735061). It is
CC found that LC-MS/MS performs well in detecting these peptides in
CC prepared model chocolates spiked with hazelnut. The sensitivity level
CC is approximately 1 mg/kg (PubMed:24577577). A stable peptide (residues
CC 395-403) from this protein is identified by MALDI-MS and it can be used
CC as an analytical target for the development of robust quantitative
CC analytical methods. This peptide is identifiable even when the protein
CC goes through a number of changes at the molecular level, such as
CC denaturation, the Maillard reaction and oxidation, reactions that
CC typically occur during food processing (PubMed:22966848). A
CC quantitative method is developed, where the coding DNA of this protein
CC is first extracted based on hexadecyltrimethylammonium bromide (CTAB)-
CC phenol-chloroform method and then detected by Real-Time PCR (RT-PCR) in
CC commercial foods such as chocolates, biscuits, cereal snacks, cookies
CC and nougat with 100% specificity and with 1 ppm sensitivity limit of
CC detection of raw hazelnut. The reliability of the method is even more
CC improved if two other hazelnut target genes (allergens Cor a 9 and Cor
CC a 13) are amplified in addition to this one. The detection is proven to
CC be accurate even when the quality and quantity of the DNA is greatly
CC diminished by roasting and autoclaving. High-hydrostatic pressure
CC treatment has no effect on DNA (PubMed:23411333). The transcription
CC level of the gene encoding this protein is investigated by a relative
CC quantitative RT-PCR technique in order to compare the transcript
CC amounts between different cultivars, and in one of the cultivars also
CC in relation to different years of harvest and ripening stages. Each
CC hazelnut sample is classified by the Principal Components Analysis
CC (PCA) to better interpret the results. The method may help in choosing
CC hypoallergenic genotypes of hazelnut for both growers and consumers
CC (Ref.12). Cold acetone extraction of proteins followed by in-solution
CC tryptic digestion and MALDI-TOF-MS (in alpha-cyano-4-chlorocinnamic
CC acid matrix) is found as a rapid and sensitive method to detect
CC residual amounts of hazelnut proteins in extra virgin olive oil
CC samples, which have been adulterated with cold-pressed hazelnut oil.
CC Peptides from this protein can be used as stable markers in this
CC technique serving as a direct proof of illegal hazelnut existence in
CC olive oil (PubMed:25209075). {ECO:0000269|PubMed:21735061,
CC ECO:0000269|PubMed:22966848, ECO:0000269|PubMed:23411333,
CC ECO:0000269|PubMed:24577577, ECO:0000269|PubMed:25209075,
CC ECO:0000269|Ref.12}.
CC -!- SIMILARITY: Belongs to the 7S seed storage protein family.
CC {ECO:0000305}.
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DR EMBL; AF441864; AAL86739.1; -; mRNA.
DR PDB; 6L4C; X-ray; 3.19 A; A/B/C=25-448.
DR PDBsum; 6L4C; -.
DR AlphaFoldDB; Q8S4P9; -.
DR SMR; Q8S4P9; -.
DR Allergome; 3216; Cor a 11.0101.
DR Allergome; 690; Cor a 11.
DR PRIDE; Q8S4P9; -.
DR GO; GO:0043245; C:extraorganismal space; IDA:UniProtKB.
DR GO; GO:0005507; F:copper ion binding; IDA:UniProtKB.
DR GO; GO:0045735; F:nutrient reservoir activity; IDA:UniProtKB.
DR GO; GO:0034214; P:protein hexamerization; IDA:UniProtKB.
DR GO; GO:0070207; P:protein homotrimerization; IDA:UniProtKB.
DR Gene3D; 2.60.120.10; -; 2.
DR InterPro; IPR006045; Cupin_1.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR InterPro; IPR011051; RmlC_Cupin_sf.
DR Pfam; PF00190; Cupin_1; 2.
DR SMART; SM00835; Cupin_1; 2.
DR SUPFAM; SSF51182; SSF51182; 2.
PE 1: Evidence at protein level;
KW 3D-structure; Allergen; Copper; Direct protein sequencing; Glycoprotein;
KW Metal-binding; Repeat; Seed storage protein; Storage protein.
FT CHAIN 1..448
FT /note="Vicilin Cor a 11.0101"
FT /id="PRO_0000451919"
FT DOMAIN 84..220
FT /note="Cupin type-1 1"
FT /evidence="ECO:0000255"
FT DOMAIN 263..418
FT /note="Cupin type-1 2"
FT /evidence="ECO:0000255"
FT REGION 1..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 333
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000269|PubMed:31753489,
FT ECO:0007744|PDB:6L4C"
FT BINDING 335
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000269|PubMed:31753489,
FT ECO:0007744|PDB:6L4C"
FT BINDING 362
FT /ligand="Cu cation"
FT /ligand_id="ChEBI:CHEBI:23378"
FT /evidence="ECO:0000269|PubMed:31753489,
FT ECO:0007744|PDB:6L4C"
FT SITE 47..48
FT /note="Cleavage"
FT /evidence="ECO:0000305|PubMed:19006093,
FT ECO:0000305|PubMed:31753489"
FT SITE 85
FT /note="Not glycosylated"
FT /evidence="ECO:0000269|PubMed:15233621"
FT CARBOHYD 47
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 301
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498,
FT ECO:0000269|PubMed:15233621"
FT CONFLICT 49
FT /note="S -> E (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 55
FT /note="K -> E (in Ref. 1; AA sequence and 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 58
FT /note="E -> D (in Ref. 1; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 59
FT /note="E -> N (in Ref. 1; AA sequence and 4; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 64
FT /note="F -> L (in Ref. 4; AA sequence)"
FT /evidence="ECO:0000305"
FT HELIX 66..68
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 69..75
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 78..83
FT /evidence="ECO:0007829|PDB:6L4C"
FT HELIX 94..96
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 100..106
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 110..128
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 130..135
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 137..145
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 149..153
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 159..162
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 166..168
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 170..181
FT /evidence="ECO:0007829|PDB:6L4C"
FT TURN 194..196
FT /evidence="ECO:0007829|PDB:6L4C"
FT HELIX 199..202
FT /evidence="ECO:0007829|PDB:6L4C"
FT HELIX 205..212
FT /evidence="ECO:0007829|PDB:6L4C"
FT HELIX 216..222
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 230..233
FT /evidence="ECO:0007829|PDB:6L4C"
FT TURN 238..242
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 257..259
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 263..265
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 271..274
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 277..282
FT /evidence="ECO:0007829|PDB:6L4C"
FT HELIX 284..286
FT /evidence="ECO:0007829|PDB:6L4C"
FT HELIX 290..292
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 295..302
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 306..315
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 317..325
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 327..336
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 339..341
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 344..350
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 355..358
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 364..368
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 370..372
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 374..382
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 388..393
FT /evidence="ECO:0007829|PDB:6L4C"
FT TURN 397..400
FT /evidence="ECO:0007829|PDB:6L4C"
FT HELIX 403..410
FT /evidence="ECO:0007829|PDB:6L4C"
FT HELIX 414..417
FT /evidence="ECO:0007829|PDB:6L4C"
FT HELIX 418..420
FT /evidence="ECO:0007829|PDB:6L4C"
FT STRAND 428..431
FT /evidence="ECO:0007829|PDB:6L4C"
SQ SEQUENCE 448 AA; 50856 MW; D748661592AA55F0 CRC64;
MLPKEDPELK KCKHKCRDER QFDEQQRRDG KQICEEKARE RQQEEGNSSE ESYGKEQEEN
PYVFQDEHFE SRVKTEEGRV QVLENFTKRS RLLSGIENFR LAILEANPHT FISPAHFDAE
LVLFVAKGRA TITMVREEKR ESFNVEHGDI IRIPAGTPVY MINRDENEKL FIVKILQPVS
APGHFEAFYG AGGEDPESFY RAFSWEVLEA ALKVRREQLE KVFGEQSKGS IVKASREKIR
ALSQHEEGPP RIWPFGGESS GPINLLHKHP SQSNQFGRLY EAHPDDHKQL QDLDLMVSFA
NITKGSMAGP YYNSRATKIS VVVEGEGFFE MACPHLSSSS GSYQKISARL RRGVVFVAPA
GHPVAVIASQ NNNLQVLCFE VNAHGNSRFP LAGKGNIVNE FERDAKELAF NLPSREVERI
FKNQDQAFFF PGPNKQQEEG GRGGRAFE
