ID   VCL_VICFA               Reviewed;         463 AA.
AC   P08438;
DT   01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1988, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Vicilin;
DE   Flags: Precursor;
OS   Vicia faba (Broad bean) (Faba vulgaris).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC   NPAAA clade; Hologalegina; IRL clade; Fabeae; Vicia.
OX   NCBI_TaxID=3906;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Minor;
RX   PubMed=3697075; DOI=10.1093/nar/15.23.10065;
RA   Weschke W., Baeumlein H., Wobus U.;
RT   "Nucleotide sequence of a field bean (Vicia faba L.var.minor) vicilin
RT   gene.";
RL   Nucleic Acids Res. 15:10065-10065(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Fribo;
RX   PubMed=3684610; DOI=10.1093/nar/15.22.9609;
RA   Bassuener R., van Nong H., Jung R., Saalbach G., Muentz K.;
RT   "The primary structure of the predominating vicilin storage protein subunit
RT   from field bean seeds (Vicia faba L. var. minor cv. Fribo).";
RL   Nucleic Acids Res. 15:9609-9609(1987).
CC   -!- FUNCTION: Seed storage protein.
CC   -!- SUBCELLULAR LOCATION: Vacuole, aleurone grain. Vacuole.
CC       Note=Cotyledonary membrane-bound vacuolar protein bodies.
CC   -!- SIMILARITY: Belongs to the 7S seed storage protein family.
CC       {ECO:0000305}.
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DR   EMBL; Y00506; CAA68559.1; -; Genomic_DNA.
DR   EMBL; Y00462; CAA68525.1; -; mRNA.
DR   PIR; A27288; A27288.
DR   PIR; S06309; S06309.
DR   AlphaFoldDB; P08438; -.
DR   SMR; P08438; -.
DR   GO; GO:0033095; C:aleurone grain; IEA:UniProtKB-SubCell.
DR   GO; GO:0005773; C:vacuole; IEA:UniProtKB-SubCell.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.10; -; 2.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 2.
DR   SMART; SM00835; Cupin_1; 2.
DR   SUPFAM; SSF51182; SSF51182; 2.
PE   2: Evidence at transcript level;
KW   Seed storage protein; Signal; Storage protein; Vacuole.
FT   SIGNAL          1..27
FT   CHAIN           28..463
FT                   /note="Vicilin"
FT                   /id="PRO_0000032181"
FT   DOMAIN          35..194
FT                   /note="Cupin type-1 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          254..426
FT                   /note="Cupin type-1 2"
FT                   /evidence="ECO:0000255"
FT   REGION          235..257
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        241..255
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        257
FT                   /note="R -> K (in Ref. 2; CAA68525)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        443
FT                   /note="R -> Q (in Ref. 2; CAA68525)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   463 AA;  52694 MW;  5CD09EFDE0D5E6B5 CRC64;
     MAATTLKDSF PLLTLLGIAF LASVCLSSRS DQDNPFVFES NRFQTLFENE NGHIRLLQKF
     DQHSKLLENL QNYRLLEYKS KPHTIFLPQQ TDADFILVVL SGKAILTVLL PNDRNSFSLE
     RGDTIKLPAG TIGYLVNRDD EEDLRVLDLV IPVNRPGEPQ SFLLSGNQNQ PSILSGFSKN
     ILEASFNTDY KEIEKVLLEE HGKEKYHRRG LKDRRQRGQE ENVIVKISRK QIEELNKNAK
     SSSKKSTSSE SEPFNLRSRE PIYSNKFGKF FEITPKRNPQ LQDLNIFVNY VEINEGSLLL
     PHYNSRAIVI VTVNEGKGDF ELVGQRNENQ QGLREEYDEE KEQGEEEIRK QVQNYKAKLS
     PGDVLVIPAG YPVAIKASSN LNLVGFGINA ENNQRYFLAG EEDNVISQIH KPVKELAFPG
     SAQEVDTLLE NQKQSHFANA QPRERERGSQ EIKDHLYSIL GSF