VCX1_SCHPO
ID VCX1_SCHPO Reviewed; 412 AA.
AC O59768;
DT 04-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Vacuolar calcium ion transporter;
DE AltName: Full=Vacuolar Ca(2+)/H(+) exchanger;
GN Name=vcx1; ORFNames=SPCC1795.02c, SPCC895.01;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [2]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Has a role in promoting intracellular calcium ion
CC sequestration via the exchange of calcium ions for hydrogen ions across
CC the vacuolar membrane. Involved also in manganese ion homeostasis via
CC its uptake into the vacuole (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000250}; Multi-pass
CC membrane protein {ECO:0000250}. Endoplasmic reticulum membrane
CC {ECO:0000269|PubMed:16823372}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. {ECO:0000305}.
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DR EMBL; CU329672; CAA18637.1; -; Genomic_DNA.
DR PIR; T41141; T41141.
DR PIR; T41639; T41639.
DR RefSeq; NP_588042.1; NM_001023034.2.
DR AlphaFoldDB; O59768; -.
DR SMR; O59768; -.
DR BioGRID; 275958; 2.
DR STRING; 4896.SPCC1795.02c.1; -.
DR iPTMnet; O59768; -.
DR PaxDb; O59768; -.
DR PRIDE; O59768; -.
DR EnsemblFungi; SPCC1795.02c.1; SPCC1795.02c.1:pep; SPCC1795.02c.
DR PomBase; SPCC1795.02c; vcx1.
DR VEuPathDB; FungiDB:SPCC1795.02c; -.
DR eggNOG; KOG1397; Eukaryota.
DR HOGENOM; CLU_008721_2_1_1; -.
DR InParanoid; O59768; -.
DR OMA; EMNTEPC; -.
DR PhylomeDB; O59768; -.
DR PRO; PR:O59768; -.
DR Proteomes; UP000002485; Chromosome III.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:PomBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0000329; C:fungal-type vacuole membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0015369; F:calcium:proton antiporter activity; IBA:GO_Central.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0140146; P:calcium ion import into vacuole; NAS:PomBase.
DR GO; GO:0070588; P:calcium ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0006874; P:cellular calcium ion homeostasis; IBA:GO_Central.
DR Gene3D; 1.20.1420.30; -; 1.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR004798; CAX-like.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR PANTHER; PTHR31503; PTHR31503; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
DR TIGRFAMs; TIGR00378; cax; 1.
PE 3: Inferred from homology;
KW Calcium; Calcium transport; Endoplasmic reticulum; Ion transport;
KW Manganese; Membrane; Metal-binding; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..412
FT /note="Vacuolar calcium ion transporter"
FT /id="PRO_0000352843"
FT TOPO_DOM 1..55
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..83
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 84..104
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 105..114
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..148
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 149..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..177
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..215
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 216..236
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 237..264
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..299
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 300..320
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 321..334
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 335..355
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 356..358
FT /note="Lumenal"
FT /evidence="ECO:0000250"
FT TRANSMEM 359..379
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 380..389
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 411..412
FT /note="Lumenal"
FT /evidence="ECO:0000250"
SQ SEQUENCE 412 AA; 45108 MW; 44CC02B7AF0A46B9 CRC64;
MIERLKIAKN RLEAMNSFNF PAQDRHERAP LLGSEYDHSM ARQLSLLNVV GMTKSVLMSS
YFNLMLVFVP IGLIAGWFEW NAKSVFILNM LAIIPLASLL SFATEQLSII SGPTLGALLN
ASFGNAIELI VGVLALKRGE LRIVQSSLLG SILSNLLLVF GMCLVTTGIR REITTFNITV
AQTMIAMLAL STATILIPAT FHYSLPDNAN SENALLHVSR GTAVIVLIVY VLLLVFQLKT
HKHVCHDPSE VEEETEPRIL GLRSSIAMLA IVTVFVSLCA DYLVGSIDQL VEEVNISKTF
VGLVILPVVG NAAEHVTAIV VSYRGQMDLA LGVAIGSSIQ IALFLAPFLV IVGWIISQPL
TLYFESLETV ILFVSVFLVN YLIQDGATHW LEGVQLLALY AIVVLAFFYY PQ
