VDAC2_ARATH
ID VDAC2_ARATH Reviewed; 276 AA.
AC Q9FJX3;
DT 14-DEC-2011, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 25-MAY-2022, entry version 117.
DE RecName: Full=Mitochondrial outer membrane protein porin 2;
DE AltName: Full=Voltage-dependent anion-selective channel protein 2;
DE Short=AtVDAC2;
DE Short=VDAC-2;
GN Name=VDAC2; OrderedLocusNames=At5g67500; ORFNames=K9I9.6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9734815; DOI=10.1093/dnares/5.3.203;
RA Kotani H., Nakamura Y., Sato S., Asamizu E., Kaneko T., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VI. Sequence
RT features of the regions of 1,367,185 bp covered by 19 physically assigned
RT P1 and TAC clones.";
RL DNA Res. 5:203-216(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Kim C.J., Chen H., Quinitio C., Shinn P., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA Feldmann K.A.;
RT "Full-length cDNA from Arabidopsis thaliana.";
RL Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=cv. Landsberg erecta;
RX PubMed=17272265; DOI=10.1074/mcp.m600408-mcp200;
RA Maor R., Jones A., Nuehse T.S., Studholme D.J., Peck S.C., Shirasu K.;
RT "Multidimensional protein identification technology (MudPIT) analysis of
RT ubiquitinated proteins in plants.";
RL Mol. Cell. Proteomics 6:601-610(2007).
RN [6]
RP FUNCTION, AND INDUCTION.
RX PubMed=19582214; DOI=10.3390/ijms10062476;
RA Yan J., He H., Tong S., Zhang W., Wang J., Li X., Yang Y.;
RT "Voltage-dependent anion channel 2 of Arabidopsis thaliana (AtVDAC2) is
RT involved in ABA-mediated early seedling development.";
RL Int. J. Mol. Sci. 10:2476-2486(2009).
RN [7]
RP SUBCELLULAR LOCATION, AND INDUCTION.
RX PubMed=19326079; DOI=10.1007/s10059-009-0041-z;
RA Lee S.M., Hoang M.H., Han H.J., Kim H.S., Lee K., Kim K.E., Kim D.H.,
RA Lee S.Y., Chung W.S.;
RT "Pathogen inducible voltage-dependent anion channel (AtVDAC) isoforms are
RT localized to mitochondria membrane in Arabidopsis.";
RL Mol. Cells 27:321-327(2009).
RN [8]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GENE FAMILY, DISRUPTION
RP PHENOTYPE, AND MUTAGENESIS OF HIS-223.
RX PubMed=21705391; DOI=10.1093/jxb/err113;
RA Tateda C., Watanabe K., Kusano T., Takahashi Y.;
RT "Molecular and genetic characterization of the gene family encoding the
RT voltage-dependent anion channel in Arabidopsis.";
RL J. Exp. Bot. 62:4773-4785(2011).
RN [9]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-236, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22092075; DOI=10.1021/pr200917t;
RA Aryal U.K., Krochko J.E., Ross A.R.;
RT "Identification of phosphoproteins in Arabidopsis thaliana leaves using
RT polyethylene glycol fractionation, immobilized metal-ion affinity
RT chromatography, two-dimensional gel electrophoresis and mass
RT spectrometry.";
RL J. Proteome Res. 11:425-437(2012).
CC -!- FUNCTION: Forms a channel through the mitochondrial outer membrane that
CC allows diffusion of small hydrophilic molecules. The channel adopts an
CC open conformation at low or zero membrane potential and a closed
CC conformation at potentials above 30-40 mV. The open state has a weak
CC anion selectivity whereas the closed state is cation-selective (By
CC similarity). Involved in plant growth and development at the vegetative
CC and reproductive stages. Is important for leaf and pollen development
CC and mitochondrial membrane potential steady state. May be involved in
CC ABA-mediated early seedling development and disease resistance.
CC {ECO:0000250, ECO:0000269|PubMed:19582214,
CC ECO:0000269|PubMed:21705391}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion outer membrane
CC {ECO:0000269|PubMed:19326079, ECO:0000269|PubMed:21705391}. Note=Also
CC localized in other unidentified cellular compartments.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q9FJX3-1; Sequence=Displayed;
CC -!- TISSUE SPECIFICITY: Expressed in root tips, steles, leaves, sepals,
CC petals, stamen and pistils. {ECO:0000269|PubMed:21705391}.
CC -!- INDUCTION: By the bacterial pathogen P.syringae pv. tomato. Down-
CC regulated by abscisic acid (ABA). {ECO:0000269|PubMed:19326079,
CC ECO:0000269|PubMed:19582214}.
CC -!- DOMAIN: Consists mainly of membrane-spanning sided beta-sheets.
CC -!- DISRUPTION PHENOTYPE: Dwarf plants with lesion mimic phenotype and
CC increased expression of the pathogenesis-related genes PR1, PR2 and
CC PR5. Delayed flowering, impaired development of anthers and short
CC siliques with sterile seeds. {ECO:0000269|PubMed:21705391}.
CC -!- MISCELLANEOUS: Plants silencing VDAC2 show an ABA-insensitive phenotype
CC during early seedling development under ABA treatment.
CC -!- SIMILARITY: Belongs to the eukaryotic mitochondrial porin (TC 1.B.8.1)
CC family. {ECO:0000305}.
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DR EMBL; AB013390; BAB08458.1; -; Genomic_DNA.
DR EMBL; CP002688; ANM69845.1; -; Genomic_DNA.
DR EMBL; BT025802; ABF83692.1; -; mRNA.
DR EMBL; AY085100; AAM61654.1; -; mRNA.
DR RefSeq; NP_201551.1; NM_126150.5. [Q9FJX3-1]
DR AlphaFoldDB; Q9FJX3; -.
DR SMR; Q9FJX3; -.
DR BioGRID; 22128; 7.
DR STRING; 3702.AT5G67500.2; -.
DR iPTMnet; Q9FJX3; -.
DR PRIDE; Q9FJX3; -.
DR ProteomicsDB; 243194; -. [Q9FJX3-1]
DR EnsemblPlants; AT5G67500.3; AT5G67500.3; AT5G67500. [Q9FJX3-1]
DR GeneID; 836886; -.
DR Gramene; AT5G67500.3; AT5G67500.3; AT5G67500. [Q9FJX3-1]
DR KEGG; ath:AT5G67500; -.
DR Araport; AT5G67500; -.
DR eggNOG; KOG3126; Eukaryota.
DR HOGENOM; CLU_069937_0_0_1; -.
DR InParanoid; Q9FJX3; -.
DR OMA; IRASYIH; -.
DR PhylomeDB; Q9FJX3; -.
DR PRO; PR:Q9FJX3; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FJX3; baseline and differential.
DR Genevisible; Q9FJX3; AT.
DR GO; GO:0005741; C:mitochondrial outer membrane; IBA:GO_Central.
DR GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR GO; GO:0008308; F:voltage-gated anion channel activity; IBA:GO_Central.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR CDD; cd07306; Porin3_VDAC; 1.
DR Gene3D; 2.40.160.10; -; 1.
DR InterPro; IPR023614; Porin_dom_sf.
DR InterPro; IPR001925; Porin_Euk.
DR InterPro; IPR027246; Porin_Euk/Tom40.
DR PANTHER; PTHR11743; PTHR11743; 1.
DR Pfam; PF01459; Porin_3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Growth regulation; Ion transport; Membrane;
KW Mitochondrion; Mitochondrion outer membrane; Phosphoprotein; Porin;
KW Reference proteome; Transmembrane; Transmembrane beta strand; Transport.
FT CHAIN 1..276
FT /note="Mitochondrial outer membrane protein porin 2"
FT /id="PRO_0000414081"
FT MOD_RES 76
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q9SMX3"
FT MOD_RES 236
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:22092075"
FT MUTAGEN 223
FT /note="H->P: Alteration of subcellular localization."
FT /evidence="ECO:0000269|PubMed:21705391"
SQ SEQUENCE 276 AA; 29595 MW; C0B0B35525D7D888 CRC64;
MSKGPGLFTD IGKKAKDLLT RDYNSDQKFS ISTYSASGVA LTSTALKKGG VHAADVATQY
KYKNALFDVK IDTDSSVLTT VTLTEILPST KAIASFKVPD YNSAKLEVQY FHDHATVTAA
AALKQNPLID ITATLGSPVI SFGAEAGYDT TSKTFTKYNA GISVTKPDAC LSIILGDKGD
SLKASYLHHF DEFKRTAAVG EVYRKFSTNE NTITVGGLYA IDHSTAVKAK LNNHGTLGAL
LQHEVLPRSL VTVSSEIDTK ALEKHPRFGL SLALKP
