ID   VDH_PSEFL               Reviewed;         482 AA.
AC   O69763;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-AUG-1998, sequence version 1.
DT   03-AUG-2022, entry version 80.
DE   RecName: Full=Vanillin dehydrogenase;
DE            EC=1.2.1.67;
GN   Name=vdh;
OS   Pseudomonas fluorescens.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=294;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND IDENTIFICATION.
RC   STRAIN=AN103;
RX   PubMed=9461612; DOI=10.1074/jbc.273.7.4163;
RA   Gasson M.J., Kitamura Y., McLauchlan W.R., Narbad A., Parr A.J.,
RA   Parsons E.L., Payne J., Rhodes M.J., Walton N.J.;
RT   "Metabolism of ferulic acid to vanillin. A bacterial gene of the enoyl-SCoA
RT   hydratase/isomerase superfamily encodes an enzyme for the hydration and
RT   cleavage of a hydroxycinnamic acid SCoA thioester.";
RL   J. Biol. Chem. 273:4163-4170(1998).
CC   -!- FUNCTION: Catalyzes the NAD-dependent oxidation of vanillin to vanillic
CC       acid. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + NAD(+) + vanillin = 2 H(+) + NADH + vanillate;
CC         Xref=Rhea:RHEA:13309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16632, ChEBI:CHEBI:18346, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.2.1.67;
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; Y13067; CAA73503.1; -; Genomic_DNA.
DR   AlphaFoldDB; O69763; -.
DR   SMR; O69763; -.
DR   STRING; 690597.JH730982_gene2033; -.
DR   eggNOG; COG1012; Bacteria.
DR   GO; GO:0050608; F:vanillin dehydrogenase activity; IEA:UniProtKB-EC.
DR   Gene3D; 3.40.309.10; -; 1.
DR   Gene3D; 3.40.605.10; -; 1.
DR   InterPro; IPR016161; Ald_DH/histidinol_DH.
DR   InterPro; IPR016163; Ald_DH_C.
DR   InterPro; IPR029510; Ald_DH_CS_GLU.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH_dom.
DR   Pfam; PF00171; Aldedh; 1.
DR   SUPFAM; SSF53720; SSF53720; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   3: Inferred from homology;
KW   NAD; Oxidoreductase.
FT   CHAIN           1..482
FT                   /note="Vanillin dehydrogenase"
FT                   /id="PRO_0000418953"
FT   ACT_SITE        250
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10007"
FT   ACT_SITE        284
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10007"
FT   BINDING         228..233
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   482 AA;  50440 MW;  C803EE29ECC9571B CRC64;
     MLDVPLLIGG QSCPARDGRT FERRNPVTGE LVSRVAAATL EDADAAVAAA QQAFPAWAAL
     APNERRSRLL KAAEQLQARS GEFIEAAGET GAMANWYGFN VRLAANMLRE AASMTTQVNG
     EVIPSDVPGS FAMALRQPCG VVLGIAPWNA PVILATRAIA MPLACGNTVV LKASELSPAV
     HRLIGQVLQD AGLGDGVVNV ISNAPADAAQ IVERLIANPA VRRVNFTGST HVGRIVGELS
     ARHLKPALLE LGGKAPLLVL DDADLEAAVQ AAAFGAYFNQ GQICMSTERL IVDAKVADAF
     VAQLAAKVET LRAGDPADPE SVLGSLVDAS AGTRIKALID DAVAKGARLV IGGQLEGSIL
     QPTLLDGVDA SMRLYREESF GPVAVVLRGE GEEALLQLAN DSEFGLSAAI FSRDTGRALA
     LAQRVESGIC HINGPTVHDE AQMPFGGVKS SGYGSFGGKA SIEHFTQLRW VTLQNGPRHY
     PI