ID   VDH_PSEUH               Reviewed;         481 AA.
AC   O05619;
DT   05-SEP-2012, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   03-AUG-2022, entry version 81.
DE   RecName: Full=Vanillin dehydrogenase;
DE            EC=1.2.1.67;
GN   Name=vdh;
OS   Pseudomonas sp. (strain HR199 / DSM 7063).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=86003;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX   PubMed=9098058; DOI=10.1128/jb.179.8.2595-2607.1997;
RA   Priefert H., Rabenhorst J., Steinbuechel A.;
RT   "Molecular characterization of genes of Pseudomonas sp. strain HR199
RT   involved in bioconversion of vanillin to protocatechuate.";
RL   J. Bacteriol. 179:2595-2607(1997).
CC   -!- FUNCTION: Catalyzes the NAD-dependent oxidation of vanillin to vanillic
CC       acid. {ECO:0000269|PubMed:9098058}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + NAD(+) + vanillin = 2 H(+) + NADH + vanillate;
CC         Xref=Rhea:RHEA:13309, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:16632, ChEBI:CHEBI:18346, ChEBI:CHEBI:57540,
CC         ChEBI:CHEBI:57945; EC=1.2.1.67;
CC         Evidence={ECO:0000269|PubMed:9098058};
CC   -!- SIMILARITY: Belongs to the aldehyde dehydrogenase family.
CC       {ECO:0000305}.
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DR   EMBL; Y11520; CAA72286.1; -; Genomic_DNA.
DR   AlphaFoldDB; O05619; -.
DR   SMR; O05619; -.
DR   BioCyc; MetaCyc:MON-17594; -.
DR   GO; GO:0050608; F:vanillin dehydrogenase activity; IDA:CACAO.
DR   Gene3D; 3.40.309.10; -; 1.
DR   Gene3D; 3.40.605.10; -; 1.
DR   InterPro; IPR016161; Ald_DH/histidinol_DH.
DR   InterPro; IPR016163; Ald_DH_C.
DR   InterPro; IPR029510; Ald_DH_CS_GLU.
DR   InterPro; IPR016162; Ald_DH_N.
DR   InterPro; IPR015590; Aldehyde_DH_dom.
DR   Pfam; PF00171; Aldedh; 1.
DR   SUPFAM; SSF53720; SSF53720; 1.
DR   PROSITE; PS00687; ALDEHYDE_DEHYDR_GLU; 1.
PE   1: Evidence at protein level;
KW   NAD; Oxidoreductase.
FT   CHAIN           1..481
FT                   /note="Vanillin dehydrogenase"
FT                   /id="PRO_0000418952"
FT   ACT_SITE        250
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10007"
FT   ACT_SITE        284
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10007"
FT   BINDING         228..233
FT                   /ligand="NAD(+)"
FT                   /ligand_id="ChEBI:CHEBI:57540"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   481 AA;  50837 MW;  9661EF2DF0F6D59E CRC64;
     MFHVPLLIGG KPCSASDERT FERRSPLTGE VVSRVAAASL EDADAAVAAA QAAFPEWAAL
     APSERRARLL RAADLLEDRS SEFTAAASET GAAGNWYGFN VYLAAGMLRE AAAMTTQIQG
     DVIPSNVPGS FAMAVRQPCG VVLGIAPWNA PVILGVRAVA MPLACGNTVV LKSSELSPFT
     HRLIGQVLHD AGLGDGVVNV ISNAPQDAPA VVERLIANPA VRRVNFTGST HVGRIIGELS
     ARHLKPAVLE LGGKAPFLVL DDADLDAAVE AAAFGAYFNQ GQICMSTERL IVTAVADAFV
     EKLARKVATL RAGDPNDPQS VLGSLIDANA GQRIQVLVDD ALAKGARQVV GGGLDGSIMQ
     PMLLDQVTEE MRLYREESFG PVAVVLRGDG DEELLRLAND SEFGLSAAIF SRDVSRAMEL
     AQRVDSGICH INGPTVHDEA QMPFGGVKSS GYGSFGSRAS IEHFTQLRWL TIQNGPRHYP
     I