VEA_DOTSN
ID VEA_DOTSN Reviewed; 611 AA.
AC N1PZ58;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 26-JUN-2013, sequence version 1.
DT 25-MAY-2022, entry version 23.
DE RecName: Full=Developmental and secondary metabolism regulator veA {ECO:0000305};
DE AltName: Full=Velvet complex subunit A {ECO:0000305};
GN Name=veA {ECO:0000303|PubMed:22227160}; ORFNames=DOTSEDRAFT_69562;
OS Dothistroma septosporum (strain NZE10 / CBS 128990) (Red band needle blight
OS fungus) (Mycosphaerella pini).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Dothideomycetidae; Mycosphaerellales; Mycosphaerellaceae; Dothistroma.
OX NCBI_TaxID=675120;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NZE10 / CBS 128990;
RX PubMed=23209441; DOI=10.1371/journal.pgen.1003088;
RA de Wit P.J.G.M., van der Burgt A., Oekmen B., Stergiopoulos I.,
RA Abd-Elsalam K.A., Aerts A.L., Bahkali A.H., Beenen H.G., Chettri P.,
RA Cox M.P., Datema E., de Vries R.P., Dhillon B., Ganley A.R.,
RA Griffiths S.A., Guo Y., Hamelin R.C., Henrissat B., Kabir M.S.,
RA Jashni M.K., Kema G., Klaubauf S., Lapidus A., Levasseur A., Lindquist E.,
RA Mehrabi R., Ohm R.A., Owen T.J., Salamov A., Schwelm A., Schijlen E.,
RA Sun H., van den Burg H.A., van Ham R.C.H.J., Zhang S., Goodwin S.B.,
RA Grigoriev I.V., Collemare J., Bradshaw R.E.;
RT "The genomes of the fungal plant pathogens Cladosporium fulvum and
RT Dothistroma septosporum reveal adaptation to different hosts and lifestyles
RT but also signatures of common ancestry.";
RL PLoS Genet. 8:E1003088-E1003088(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NZE10 / CBS 128990;
RX PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA Grigoriev I.V.;
RT "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT genomes of eighteen Dothideomycetes fungi.";
RL PLoS Pathog. 8:E1003037-E1003037(2012).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=22227160; DOI=10.1016/j.fgb.2011.11.009;
RA Chettri P., Calvo A.M., Cary J.W., Dhingra S., Guo Y., McDougal R.L.,
RA Bradshaw R.E.;
RT "The veA gene of the pine needle pathogen Dothistroma septosporum regulates
RT sporulation and secondary metabolism.";
RL Fungal Genet. Biol. 49:141-151(2012).
CC -!- FUNCTION: Component of the velvet transcription factor complex that
CC controls sexual/asexual developmental ratio in response to light,
CC promoting sexual development in the darkness while stimulating asexual
CC sporulation under illumination (By similarity). The velvet complex hat
CC acts as a global regulator for secondary metabolite gene expression
CC (PubMed:22227160). Controls the expression of the dothistromin gene
CC cluster (PubMed:22227160). Regulates hyphal growth and pigment
CC formation (PubMed:22227160). Acts as a positive regulator of virulence
CC (PubMed:22227160). {ECO:0000250|UniProtKB:C8VTV4,
CC ECO:0000269|PubMed:22227160}.
CC -!- SUBUNIT: Component of the heterotrimeric velvet complex composed of
CC laeA, veA and velB; VeA acting as a bridging protein between laeA and
CC velB (By similarity). {ECO:0000250|UniProtKB:C8VQG9}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C8VTV4}. Cytoplasm
CC {ECO:0000250|UniProtKB:C8VTV4}. Note=Enriched in the nucleus in the
CC dark (By similarity). {ECO:0000250|UniProtKB:C8VTV4}.
CC -!- DOMAIN: The C-terminal PEST domain is a region rich in proline,
CC glutamic acid, serine and threonine residues that is required for the
CC light-dependent regulation of development and secondary metabolism (By
CC similarity). {ECO:0000250|UniProtKB:C8VTV4}.
CC -!- DISRUPTION PHENOTYPE: Impairs the biosynthesis of secondary
CC metabolites, including dothistromin (PubMed:22227160). Impairs asexual
CC sporulation but shows normal hydrophobicity (PubMed:22227160).
CC {ECO:0000269|PubMed:22227160}.
CC -!- SIMILARITY: Belongs to the velvet family. VeA subfamily. {ECO:0000305}.
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DR EMBL; KB446536; EME47645.1; -; Genomic_DNA.
DR AlphaFoldDB; N1PZ58; -.
DR SMR; N1PZ58; -.
DR EnsemblFungi; EME47645; EME47645; DOTSEDRAFT_69562.
DR eggNOG; ENOG502S0HV; Eukaryota.
DR HOGENOM; CLU_022491_2_0_1; -.
DR OMA; NHFVTHR; -.
DR OrthoDB; 1171722at2759; -.
DR Proteomes; UP000016933; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.3960; -; 1.
DR InterPro; IPR021740; Velvet.
DR InterPro; IPR037525; Velvet_dom.
DR InterPro; IPR038491; Velvet_dom_sf.
DR PANTHER; PTHR33572; PTHR33572; 1.
DR Pfam; PF11754; Velvet; 2.
DR PROSITE; PS51821; VELVET; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Nucleus; Reference proteome; Sporulation; Transcription;
KW Transcription regulation.
FT CHAIN 1..611
FT /note="Developmental and secondary metabolism regulator
FT veA"
FT /id="PRO_0000435770"
FT DOMAIN 24..216
FT /note="Velvet"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01165"
FT REGION 1..57
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 222..497
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 455..499
FT /note="PEST"
FT /evidence="ECO:0000250|UniProtKB:C8VTV4"
FT REGION 511..611
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 38..43
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250|UniProtKB:C8VTV4"
FT COMPBIAS 11..43
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..242
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 272..323
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 324..365
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 385..401
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 415..447
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 511..534
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 550..579
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 611 AA; 67573 MW; 63F2694D9B4E0BC5 CRC64;
MNRKTHIPVE NETKSSATRT TNDGRAITYE MQVLQQPQRA RACGQGAKSS ADRRPVDPPP
IVELKIFEGE KRDDITYTMH ANYFLFATLE QARPIAHARG QDRNTHPVLT GTPVAGMVYL
DRPTPAGYFI FPDLSVRHEG EYRLSFSLYE ELKNPKDEDK PEEACDAAGG DAHVTHRLEV
KSAPFHVYSA KKFPGLTEST HLSRMVAEQG CRVRIRRDVR MRRREPKSGG KDWDEYEEDT
AAARARASAT PDPSINGYMQ TPHGFIEPNP RPRSASNASH QSLGSISRRP SMQEMGQAYH
QQPHYGTAPH TPQNGYTQTA PYGPPPGQQY PPNQFVQQQP PMQPPLPQYQ PPNYPAPPPP
VTAAQQPQPA QSYYNYPAAP PPPQATQVQQ YNVSAHAYDS GVQSHRPSID FPAQDGYRRN
SQQIPPTSQP TAYTQPMQPQ YAAQMPPAQH YQQPPPPPPS QASQHSSYSS MDLYNSRPAP
IEPHHHGNTP ASKASFDLPP INTAAMVSNK LEASSPTSVA PTNAYFSGGQ TPIDTHKRSY
GDVFSNRHHN APLRQGQRPS YGQGDSLVTG TTMSAADDDD NASSELDPST LGMHYRRADG
RQIQRALPGH A
