ID   VEA_HYPVG               Reviewed;         541 AA.
AC   G9NAW2;
DT   16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT   22-FEB-2012, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Developmental and secondary metabolism regulator VEL1 {ECO:0000305};
DE   AltName: Full=Velvet complex subunit 1 {ECO:0000305};
GN   Name=VEL1 {ECO:0000303|PubMed:20154111}; ORFNames=TRIVIDRAFT_164251;
OS   Hypocrea virens (strain Gv29-8 / FGSC 10586) (Gliocladium virens)
OS   (Trichoderma virens).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Hypocreaceae; Trichoderma.
OX   NCBI_TaxID=413071;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Gv29-8 / FGSC 10586;
RX   PubMed=21501500; DOI=10.1186/gb-2011-12-4-r40;
RA   Kubicek C.P., Herrera-Estrella A., Seidl-Seiboth V., Martinez D.A.,
RA   Druzhinina I.S., Thon M., Zeilinger S., Casas-Flores S., Horwitz B.A.,
RA   Mukherjee P.K., Mukherjee M., Kredics L., Alcaraz L.D., Aerts A., Antal Z.,
RA   Atanasova L., Cervantes-Badillo M.G., Challacombe J., Chertkov O.,
RA   McCluskey K., Coulpier F., Deshpande N., von Doehren H., Ebbole D.J.,
RA   Esquivel-Naranjo E.U., Fekete E., Flipphi M., Glaser F.,
RA   Gomez-Rodriguez E.Y., Gruber S., Han C., Henrissat B., Hermosa R.,
RA   Hernandez-Onate M., Karaffa L., Kosti I., Le Crom S., Lindquist E.,
RA   Lucas S., Luebeck M., Luebeck P.S., Margeot A., Metz B., Misra M.,
RA   Nevalainen H., Omann M., Packer N., Perrone G., Uresti-Rivera E.E.,
RA   Salamov A., Schmoll M., Seiboth B., Shapiro H., Sukno S.,
RA   Tamayo-Ramos J.A., Tisch D., Wiest A., Wilkinson H.H., Zhang M.,
RA   Coutinho P.M., Kenerley C.M., Monte E., Baker S.E., Grigoriev I.V.;
RT   "Comparative genome sequence analysis underscores mycoparasitism as the
RT   ancestral life style of Trichoderma.";
RL   Genome Biol. 12:R40.1-R40.15(2011).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=20154111; DOI=10.1128/aem.02391-09;
RA   Mukherjee P.K., Kenerley C.M.;
RT   "Regulation of morphogenesis and biocontrol properties in Trichoderma
RT   virens by a VELVET protein, Vel1.";
RL   Appl. Environ. Microbiol. 76:2345-2352(2010).
CC   -!- FUNCTION: Component of the velvet transcription factor complex that
CC       controls sexual/asexual developmental ratio in response to light,
CC       promoting sexual development in the darkness while stimulating asexual
CC       sporulation under illumination (By similarity). The velvet complex acts
CC       as a global regulator for secondary metabolite gene expression
CC       (PubMed:20154111). Controls the expression of the gliotoxin gene
CC       cluster (PubMed:20154111). Plays a key role in mycoparasitism
CC       (PubMed:20154111). {ECO:0000250|UniProtKB:C8VTV4,
CC       ECO:0000269|PubMed:20154111}.
CC   -!- SUBUNIT: Component of the heterotrimeric velvet complex composed of
CC       LAE1, VEL1 and VEL2; VEL1 acting as a bridging protein between LAE1 and
CC       VEL2 (By similarity). {ECO:0000250|UniProtKB:C8VTV4}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C8VTV4}. Cytoplasm
CC       {ECO:0000250|UniProtKB:C8VTV4}. Note=Enriched in the nucleus in the
CC       dark (By similarity). {ECO:0000250|UniProtKB:C8VTV4}.
CC   -!- DOMAIN: The C-terminal PEST domain is a region rich in proline,
CC       glutamic acid, serine and threonine residues that is required for the
CC       light-dependent regulation of development and secondary metabolism (By
CC       similarity). {ECO:0000250|UniProtKB:C8VTV4}.
CC   -!- DISRUPTION PHENOTYPE: Exhibit early chlamydospore differentiation under
CC       nutrient stress conditions (PubMed:20154111). Impairs gliotoxin
CC       production and induction of other secondary metabolism-related genes
CC       including genes encoding nonribosomal peptide synthetases, 2 polyketide
CC       synthases, an O-methyl transferase, and cytochrome P450
CC       (PubMed:20154111). Impairs mycoparasitism (PubMed:20154111).
CC       {ECO:0000269|PubMed:20154111}.
CC   -!- SIMILARITY: Belongs to the velvet family. VeA subfamily. {ECO:0000305}.
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DR   EMBL; ABDF02000091; EHK16344.1; -; Genomic_DNA.
DR   RefSeq; XP_013950537.1; XM_014095062.1.
DR   AlphaFoldDB; G9NAW2; -.
DR   SMR; G9NAW2; -.
DR   PRIDE; G9NAW2; -.
DR   EnsemblFungi; EHK16344; EHK16344; TRIVIDRAFT_164251.
DR   GeneID; 25788603; -.
DR   VEuPathDB; FungiDB:TRIVIDRAFT_164251; -.
DR   eggNOG; ENOG502S0HV; Eukaryota.
DR   HOGENOM; CLU_022491_2_0_1; -.
DR   InParanoid; G9NAW2; -.
DR   OMA; NFFLYAT; -.
DR   OrthoDB; 1171722at2759; -.
DR   Proteomes; UP000007115; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.40.3960; -; 1.
DR   InterPro; IPR021740; Velvet.
DR   InterPro; IPR037525; Velvet_dom.
DR   InterPro; IPR038491; Velvet_dom_sf.
DR   PANTHER; PTHR33572; PTHR33572; 1.
DR   Pfam; PF11754; Velvet; 2.
DR   PROSITE; PS51821; VELVET; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Nucleus; Reference proteome; Sporulation; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..541
FT                   /note="Developmental and secondary metabolism regulator
FT                   VEL1"
FT                   /id="PRO_0000435777"
FT   DOMAIN          26..220
FT                   /note="Velvet"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01165"
FT   REGION          222..447
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          444..472
FT                   /note="PEST"
FT                   /evidence="ECO:0000250|UniProtKB:C8VTV4"
FT   REGION          464..483
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           40..45
FT                   /note="Nuclear localization signal"
FT                   /evidence="ECO:0000250|UniProtKB:C8VTV4"
FT   COMPBIAS        226..248
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        295..312
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        339..362
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        402..419
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        424..442
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        466..480
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   541 AA;  59478 MW;  1C9576FC9F8DD549 CRC64;
     MATPTSVASS ASRDMVQRIH RVTRENRHLW YQLTVLQQPE RARACGSGMK ANSDRRPVDP
     PPVVELRIIE GPSVEEGKDI TFDYNANFFL YASLEQARPI AHGRVQNGAT NNPPILTGVP
     ASGMAYLDRP TEAGYFIFPD LSVRHEGYFR LSFSLYETTK ESKDFDMEPA DSDLPAGVDW
     RMEIKTQPFN VFSAKKFPGL MESTSLSKTV ADQGCRVRIR RDVRMRKRDG KGSGYDRREE
     EYARRRTVTP APAEDPMRAR STSNASEHRA PYMPQDSQRR PSAAESYHAP SLPHAPSLPH
     APPPPAYDAP PPAARPGHLQ FGGEQNMPQY GGPAPPRSYA HPQGAPIPPV TPTGPYPTAS
     APSPYPKHDS QPYSYGPRPP VSSASPAPPM KHAGYDSRPS EPYAPQSPSG YTPTERRPSF
     VSYPSPAPMT PYIAPPAPSP ARHMPTQSSL APLKIASLVS PLPPIEAQTE PLPPPPLLPT
     GGKRKHDYVF SQNTKPLHNG QRQLDAHFGH GYRGLTPEPD QGLYSRADGQ IGVVTFNQYQ
     V