VEGFA_COTJA
ID VEGFA_COTJA Reviewed; 216 AA.
AC P67965; P52582; Q91420;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2004, sequence version 1.
DT 03-AUG-2022, entry version 78.
DE RecName: Full=Vascular endothelial growth factor A;
DE Short=VEGF-A;
DE AltName: Full=Vascular permeability factor;
DE Short=VPF;
DE Flags: Precursor;
GN Name=VEGFA; Synonyms=VEGF;
OS Coturnix japonica (Japanese quail) (Coturnix coturnix japonica).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Archelosauria; Archosauria; Dinosauria; Saurischia; Theropoda;
OC Coelurosauria; Aves; Neognathae; Galloanserae; Galliformes; Phasianidae;
OC Perdicinae; Coturnix.
OX NCBI_TaxID=93934;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS VEGF-122; VEGF-146; VEGF-166 AND
RP VEGF-190), AND FUNCTION.
RC TISSUE=Embryo;
RX PubMed=7556923; DOI=10.1006/dbio.1995.1291;
RA Flamme I., von Reutern M., Drexler H.C.A., Syed-Ali S., Risau W.;
RT "Overexpression of vascular endothelial growth factor in the avian embryo
RT induces hypervascularization and increased vascular permeability without
RT alterations of embryonic pattern formation.";
RL Dev. Biol. 171:399-414(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 60-187 (ISOFORMS VEGF-190 AND VEGF-166),
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=7781909; DOI=10.1006/dbio.1995.1180;
RA Flamme I., Breier G., Risau W.;
RT "Vascular endothelial growth factor (VEGF) and VEGF receptor 2 (flk-1) are
RT expressed during vasculogenesis and vascular differentiation in the quail
RT embryo.";
RL Dev. Biol. 169:699-712(1995).
CC -!- FUNCTION: Growth factor active in angiogenesis, vasculogenesis and
CC endothelial cell growth. Induces endothelial cell proliferation,
CC promotes cell migration, inhibits apoptosis and induces
CC permeabilization of blood vessels. Binds to the FLT1/VEGFR1 and
CC KDR/VEGFR2 receptors, heparan sulfate and heparin (By similarity).
CC {ECO:0000250, ECO:0000269|PubMed:7556923}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. Also found as heterodimer with
CC PGF (By similarity). {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Comment=Additional isoforms seem to exist.;
CC Name=VEGF-190;
CC IsoId=P67965-1, P52582-1;
CC Sequence=Displayed;
CC Name=VEGF-166;
CC IsoId=P67965-2, P52582-2;
CC Sequence=VSP_004633, VSP_004634;
CC Name=VEGF-146;
CC IsoId=P67965-3, P52582-3;
CC Sequence=VSP_004635, VSP_004636;
CC Name=VEGF-122;
CC IsoId=P67965-4; Sequence=VSP_018620;
CC -!- TISSUE SPECIFICITY: Widely expressed. Abundantly and equally expressed
CC in heart and liver. In kidney glomeruli, brain and yolk sac, isoform
CC VEGF-166 is 5- to 10-times more abundant than isoform VEGF-190.
CC {ECO:0000269|PubMed:7781909}.
CC -!- DEVELOPMENTAL STAGE: Isoform VEGF-166 is expressed early at day 1 and
CC increases during gastrulation. Expression of isoform VEGF-190 is
CC detectable only from day 2. {ECO:0000269|PubMed:7781909}.
CC -!- DOMAIN: Isoform VEGF-190 contains a basic insert which acts as a cell
CC retention signal.
CC -!- SIMILARITY: Belongs to the PDGF/VEGF growth factor family.
CC {ECO:0000305}.
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DR EMBL; S79680; AAB35371.1; -; mRNA.
DR RefSeq; XP_015713222.1; XM_015857736.1. [P67965-1]
DR RefSeq; XP_015713224.1; XM_015857738.1. [P67965-2]
DR RefSeq; XP_015713227.1; XM_015857741.1. [P67965-4]
DR AlphaFoldDB; P67965; -.
DR SMR; P67965; -.
DR Ensembl; ENSCJPT00005019283; ENSCJPP00005013421; ENSCJPG00005011306. [P67965-1]
DR Ensembl; ENSCJPT00005019285; ENSCJPP00005013423; ENSCJPG00005011306. [P67965-2]
DR Ensembl; ENSCJPT00005019286; ENSCJPP00005013424; ENSCJPG00005011306. [P67965-3]
DR Ensembl; ENSCJPT00005019287; ENSCJPP00005013425; ENSCJPG00005011306. [P67965-4]
DR GeneID; 107311307; -.
DR KEGG; cjo:107311307; -.
DR CTD; 7422; -.
DR GeneTree; ENSGT00940000157284; -.
DR Proteomes; UP000694412; Chromosome 3.
DR GO; GO:0009986; C:cell surface; TAS:Roslin.
DR GO; GO:0005576; C:extracellular region; NAS:Roslin.
DR GO; GO:0016020; C:membrane; IEA:InterPro.
DR GO; GO:0008083; F:growth factor activity; IDA:Roslin.
DR GO; GO:0008201; F:heparin binding; IEA:UniProtKB-KW.
DR GO; GO:0005172; F:vascular endothelial growth factor receptor binding; TAS:Roslin.
DR GO; GO:0001525; P:angiogenesis; IEA:UniProtKB-KW.
DR GO; GO:0001955; P:blood vessel maturation; IDA:Roslin.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007267; P:cell-cell signaling; TAS:Roslin.
DR GO; GO:0045766; P:positive regulation of angiogenesis; IDA:Roslin.
DR GO; GO:0051781; P:positive regulation of cell division; IEA:UniProtKB-KW.
DR GO; GO:0010595; P:positive regulation of endothelial cell migration; ISS:UniProtKB.
DR GO; GO:0001938; P:positive regulation of endothelial cell proliferation; ISS:UniProtKB.
DR GO; GO:0051894; P:positive regulation of focal adhesion assembly; ISS:UniProtKB.
DR GO; GO:0050731; P:positive regulation of peptidyl-tyrosine phosphorylation; ISS:UniProtKB.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; ISS:UniProtKB.
DR GO; GO:0031334; P:positive regulation of protein-containing complex assembly; ISS:UniProtKB.
DR GO; GO:0043117; P:positive regulation of vascular permeability; IDA:Roslin.
DR GO; GO:0035148; P:tube formation; ISS:UniProtKB.
DR GO; GO:0048010; P:vascular endothelial growth factor receptor signaling pathway; TAS:Roslin.
DR GO; GO:0001570; P:vasculogenesis; IDA:Roslin.
DR CDD; cd00135; PDGF; 1.
DR Gene3D; 2.10.160.10; -; 1.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR023581; PD_growth_factor_CS.
DR InterPro; IPR000072; PDGF/VEGF_dom.
DR InterPro; IPR027928; VEGF_C.
DR InterPro; IPR036841; VEGF_C_sf.
DR Pfam; PF00341; PDGF; 1.
DR Pfam; PF14554; VEGF_C; 1.
DR SMART; SM00141; PDGF; 1.
DR SUPFAM; SSF57501; SSF57501; 1.
DR SUPFAM; SSF57593; SSF57593; 1.
DR PROSITE; PS00249; PDGF_1; 1.
DR PROSITE; PS50278; PDGF_2; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Angiogenesis; Developmental protein; Differentiation;
KW Disulfide bond; Glycoprotein; Growth factor; Heparin-binding; Mitogen;
KW Reference proteome; Signal.
FT SIGNAL 1..26
FT /evidence="ECO:0000250"
FT CHAIN 27..216
FT /note="Vascular endothelial growth factor A"
FT /id="PRO_0000023393"
FT REGION 132..167
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 138..162
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 101
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 52..94
FT /evidence="ECO:0000250"
FT DISULFID 77
FT /note="Interchain"
FT /evidence="ECO:0000250"
FT DISULFID 83..128
FT /evidence="ECO:0000250"
FT DISULFID 86
FT /note="Interchain"
FT /evidence="ECO:0000250"
FT DISULFID 87..130
FT /evidence="ECO:0000250"
FT VAR_SEQ 142
FT /note="K -> N (in isoform VEGF-166)"
FT /evidence="ECO:0000303|PubMed:7556923,
FT ECO:0000303|PubMed:7781909"
FT /id="VSP_004633"
FT VAR_SEQ 143..210
FT /note="Missing (in isoform VEGF-122)"
FT /evidence="ECO:0000303|PubMed:7556923"
FT /id="VSP_018620"
FT VAR_SEQ 143..166
FT /note="Missing (in isoform VEGF-166)"
FT /evidence="ECO:0000303|PubMed:7556923,
FT ECO:0000303|PubMed:7781909"
FT /id="VSP_004634"
FT VAR_SEQ 166
FT /note="F -> L (in isoform VEGF-146)"
FT /evidence="ECO:0000303|PubMed:7556923"
FT /id="VSP_004635"
FT VAR_SEQ 167..210
FT /note="Missing (in isoform VEGF-146)"
FT /evidence="ECO:0000303|PubMed:7556923"
FT /id="VSP_004636"
SQ SEQUENCE 216 AA; 25203 MW; 82E669C2F6FC6DA7 CRC64;
MNFLLTWIHW GLAALLYLQS AELSKAAPAL GDGERKPNEV IKFLEVYERS FCRTIETLVD
IFQEYPDEVE YIFRPSCVPL MRCAGCCGDE GLECVPVDVY NVTMEIARIK PHQSQHIAHM
SFLQHSKCDC RPKKDVKNKQ EKKSKRGKGK GQKRKRKKGR YKPPSFHCEP CSERRKHLFV
QDPQTCKCSC KFTDSRCKSR QLELNERTCR CEKPRR
