VEGTA_XENLA
ID VEGTA_XENLA Reviewed; 455 AA.
AC P87377; A2BDB3; B1H1N4; B7ZS85; P79930; P87386;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 15-DEC-2009, sequence version 2.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=T-box protein VegT-A;
DE AltName: Full=Brachyury and Tbx-related protein;
DE Short=T-box protein Brat;
DE AltName: Full=T-box protein Antipodean;
GN Name=vegt-a; Synonyms=apod, brat, vegt;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, SUBCELLULAR LOCATION,
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RC TISSUE=Oocyte;
RX PubMed=9012531; DOI=10.1242/dev.122.12.4119;
RA Zhang J., King M.L.;
RT "Xenopus VegT RNA is localized to the vegetal cortex during oogenesis and
RT encodes a novel T-box transcription factor involved in mesodermal
RT patterning.";
RL Development 122:4119-4129(1996).
RN [2]
RP SEQUENCE REVISION TO 209 AND 396-455.
RA Zhang J., King M.L.;
RL Submitted (JAN-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), FUNCTION, TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC TISSUE=Gastrula;
RX PubMed=9012537; DOI=10.1242/dev.122.12.4179;
RA Stennard F., Carnac G., Gurdon J.B.;
RT "The Xenopus T-box gene, Antipodean, encodes a vegetally localised maternal
RT mRNA and can trigger mesoderm formation.";
RL Development 122:4179-4188(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, TISSUE SPECIFICITY,
RP DEVELOPMENTAL STAGE, AND INDUCTION.
RC TISSUE=Gastrula;
RX PubMed=9165117; DOI=10.1242/dev.124.9.1689;
RA Horb M.E., Thomsen G.H.;
RT "A vegetally localized T-box transcription factor in Xenopus eggs specifies
RT mesoderm and endoderm and is essential for embryonic mesoderm formation.";
RL Development 124:1689-1698(1997).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Gastrula, and Ovary;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (NOV-2008) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP FUNCTION.
RX PubMed=9735361; DOI=10.1242/dev.125.20.3997;
RA Tada M., Casey E.S., Fairclough L., Smith J.C.;
RT "Bix1, a direct target of Xenopus T-box genes, causes formation of ventral
RT mesoderm and endoderm.";
RL Development 125:3997-4006(1998).
RN [7]
RP INDUCTION.
RX PubMed=10068640; DOI=10.1242/dev.126.7.1467;
RA Sun B.I., Bush S.M., Collins-Racie L.A., LaVallie E.R.,
RA DiBlasio-Smith E.A., Wolfman N.M., McCoy J.M., Sive H.L.;
RT "derriere: a TGF-beta family member required for posterior development in
RT Xenopus.";
RL Development 126:1467-1482(1999).
RN [8]
RP FUNCTION.
RX PubMed=10518506; DOI=10.1242/dev.126.21.4903;
RA Clements D., Friday R.V., Woodland H.R.;
RT "Mode of action of VegT in mesoderm and endoderm formation.";
RL Development 126:4903-4911(1999).
RN [9]
RP INDUCTION.
RX PubMed=10683175; DOI=10.1242/dev.127.6.1221;
RA Hyde C.E., Old R.W.;
RT "Regulation of the early expression of the Xenopus nodal-related 1 gene,
RT Xnr1.";
RL Development 127:1221-1229(2000).
RN [10]
RP INDUCTION.
RX PubMed=12070085; DOI=10.1242/dev.129.13.3089;
RA Eimon P.M., Harland R.M.;
RT "Effects of heterodimerization and proteolytic processing on Derriere and
RT Nodal activity: implications for mesoderm induction in Xenopus.";
RL Development 129:3089-3103(2002).
RN [11]
RP TISSUE SPECIFICITY.
RX PubMed=12142022; DOI=10.1006/dbio.2002.0719;
RA Bubunenko M., Kress T.L., Vempati U.D., Mowry K.L., King M.L.;
RT "A consensus RNA signal that directs germ layer determinants to the vegetal
RT cortex of Xenopus oocytes.";
RL Dev. Biol. 248:82-92(2002).
RN [12]
RP FUNCTION, AND INTERACTION WITH TCF7L1; POU5F1.1; POU5F1.2 AND POU5F1.3.
RX PubMed=17541407; DOI=10.1038/sj.emboj.7601736;
RA Cao Y., Siegel D., Donow C., Knochel S., Yuan L., Knochel W.;
RT "POU-V factors antagonize maternal VegT activity and beta-Catenin signaling
RT in Xenopus embryos.";
RL EMBO J. 26:2942-2954(2007).
CC -!- FUNCTION: Transcription factor required for both mesoderm and endoderm
CC formation in the embryo; signaling determinants and concentration
CC levels may determine which germ layer is formed. Acts together with
CC beta-catenin to activate genes that are responsible for mesoderm
CC induction including wnt-8, eomes t/bra, siamois, mix1 and sox17.
CC Directly binds to promoter DNA. Patterns the mesoderm along the
CC dorsoventral and posterior axis. Activates siamois gene transcription
CC when alone or in combination with beta-catenin, but inhibits siamois
CC transcription in combination with pou5f1.1/oct-25.
CC {ECO:0000269|PubMed:10518506, ECO:0000269|PubMed:17541407,
CC ECO:0000269|PubMed:9012531, ECO:0000269|PubMed:9012537,
CC ECO:0000269|PubMed:9165117, ECO:0000269|PubMed:9735361}.
CC -!- SUBUNIT: Forms a repression complex on the promoters of the nodal/nr1
CC and siamois genes with the maternal factors tcf7l1/tcf3 and
CC pouf5.1/oct-25. Interacts (via C-terminus) with tcf7l1/tcf3 (via N-
CC terminus). Also interacts with the other POU-domain transcription
CC factors pou5f1.2/oct-91 and pou5f1.3/oct-60.
CC {ECO:0000269|PubMed:17541407}.
CC -!- INTERACTION:
CC P87377; Q7T103: pou5f1.1; NbExp=3; IntAct=EBI-7439000, EBI-7438970;
CC P87377; Q90ZB6: tcf7l1-b; NbExp=4; IntAct=EBI-7439000, EBI-7439087;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00201,
CC ECO:0000269|PubMed:9012531}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P87377-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P87377-2; Sequence=VSP_038540;
CC -!- TISSUE SPECIFICITY: Uniformly distributed in stage I oocytes but
CC becomes localized to the vegetal hemisphere by stage II and remains so
CC thereafter throughout oogenesis and the early embryonic cleavage
CC stages. Zygotic expression parallels blastopore formation and shifts
CC from dorsal expression in the marginal zone of late blastula and early
CC gastrula stages to a ventral/lateral expression at the posterior end of
CC later stage embryos. Expression is excluded from the notochord. In
CC tailbud and tadpole stages, expressed exclusively in a subset of
CC posterior Rohon-Beard neurons. {ECO:0000269|PubMed:12142022,
CC ECO:0000269|PubMed:9012531, ECO:0000269|PubMed:9012537,
CC ECO:0000269|PubMed:9165117}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC Maternally expressed from early oogenesis. Zygotic expression occurs
CC from late blastula and reaches maximum levels during gastrulation
CC (stages 10.5-12). Levels decline at the time of blastopore closure
CC (stage 13). {ECO:0000269|PubMed:9012531, ECO:0000269|PubMed:9012537,
CC ECO:0000269|PubMed:9165117}.
CC -!- INDUCTION: By mesoderm inducing factors and TGF-beta family members
CC including nodal/nr-1 acting in an autoregulatory loop, nodal2/nr-2
CC derriere, bFGF and activin B. {ECO:0000269|PubMed:10068640,
CC ECO:0000269|PubMed:10683175, ECO:0000269|PubMed:12070085,
CC ECO:0000269|PubMed:9165117}.
CC -!- MISCELLANEOUS: Was named Antipodean by PubMed:9012537 because of its
CC localization to the vegetal hemisphere of the egg.
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DR EMBL; U59483; AAB93301.1; -; mRNA.
DR EMBL; X99905; CAA68179.1; -; mRNA.
DR EMBL; U89707; AAB49478.1; -; mRNA.
DR EMBL; BC130162; AAI30163.1; -; mRNA.
DR EMBL; BC160675; AAI60675.1; -; mRNA.
DR EMBL; BC170433; AAI70433.1; -; mRNA.
DR EMBL; BC170435; AAI70435.1; -; mRNA.
DR RefSeq; NP_001081665.1; NM_001088196.1.
DR RefSeq; XP_018088382.1; XM_018232893.1. [P87377-2]
DR AlphaFoldDB; P87377; -.
DR SMR; P87377; -.
DR IntAct; P87377; 4.
DR MINT; P87377; -.
DR MaxQB; P87377; -.
DR GeneID; 397986; -.
DR KEGG; xla:397986; -.
DR CTD; 397986; -.
DR Xenbase; XB-GENE-6252142; vegt.L.
DR OrthoDB; 810611at2759; -.
DR Proteomes; UP000186698; Chromosome 1L.
DR Bgee; 397986; Expressed in gastrula and 7 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0005667; C:transcription regulator complex; IPI:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0140297; F:DNA-binding transcription factor binding; IPI:UniProtKB.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IDA:UniProtKB.
DR GO; GO:0001707; P:mesoderm formation; IMP:UniProtKB.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IDA:UniProtKB.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:UniProtKB.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 2.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; Developmental protein; DNA-binding;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..455
FT /note="T-box protein VegT-A"
FT /id="PRO_0000184462"
FT DNA_BIND 57..230
FT /note="T-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 21..40
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 229..262
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 295..346
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 22..40
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 229..244
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 295..340
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..25
FT /note="MRNCCRECGLSAGHLEPEASSNCAS -> MHSLP (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:9012537"
FT /id="VSP_038540"
FT CONFLICT 244
FT /note="L -> H (in Ref. 4; AAB49478)"
FT /evidence="ECO:0000305"
FT CONFLICT 350
FT /note="T -> P (in Ref. 5; AAI60675)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 455 AA; 51771 MW; CF7FAC946A1847C0 CRC64;
MRNCCRECGL SAGHLEPEAS SNCASDVKSS PDMDSVSSQD SLYLPNTVGA SLEDQDLWSQ
FHQEGTEMII TKSGRRMFPQ CKIRLFGLHP YAKYMLLVDF VPLDNFRYKW NKNQWEAAGK
AEPHPPCRTY VHPDSPAPGA HWMKDPICFQ KLKLTNNTLD QQGHIILHSM HRYKPRFHVV
QSDDMYNSPW GLVQVFSFPE TEFTSVTAYQ NEKITKLKIN HNPFAKGFRE QERSHKRDDV
LKILQQSPSK RQKRKKWEDS PEADISDFPK AICVKEESIM DPAGVYQNWV SDHEANQGLT
PHSPESEGAN QEQQVPTSSS NFYNKSHYRR SSQHLSSPFE LGEPSSRRLT PDIATVPDSD
PDSLAVFHVI PTQNSAPERT CSMNFSMEAP MKQPLRGAMY SPYGADQWLV PAQGQYRPVG
YTAYPTDLST QGAVAHPHSA MSDWSQYSLF PYSCW
