VEGT_XENTR
ID VEGT_XENTR Reviewed; 455 AA.
AC Q28HY0; B4F6G6; B7XDC3; Q98UD1;
DT 15-DEC-2009, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=T-box protein VegT {ECO:0000250|UniProtKB:P87377};
DE Short=tVegT {ECO:0000303|PubMed:12508233};
GN Name=vegt {ECO:0000312|EMBL:AAI67867.1}; ORFNames=TEgg048b09.1;
OS Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX NCBI_TaxID=8364;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAK00597.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Bubunenko M., Vempati U.D., King M.L.;
RT "Characterization of the RNA signal that directs vegetal localization of
RT VegT, the primary germ layer determinant in Xenopus.";
RL Submitted (AUG-1999) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000305, ECO:0000312|EMBL:AAK00597.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=N6 {ECO:0000312|EMBL:BAH04511.1};
RA Fukuda M., Takahashi S., Haramoto Y., Onuma Y., Yeo C., Ishiura S.,
RA Asashima M.;
RT "Eomesodermin and Nodal signaling coregulate Xenopus mesoderm formation via
RT the direct mediation of zygotic VegT expression.";
RL Submitted (AUG-2008) to the EMBL/GenBank/DDBJ databases.
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAK00597.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Egg {ECO:0000312|EMBL:CAJ82211.1};
RG Sanger Xenopus tropicalis EST/cDNA project;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000305, ECO:0000312|EMBL:AAK00597.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Gastrula {ECO:0000312|EMBL:AAI67867.1};
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (JUL-2008) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=12508233; DOI=10.1002/dvdy.10201;
RA D'Souza A., Lee M., Taverner N., Mason J., Carruthers S., Smith J.C.,
RA Amaya E., Papalopulu N., Zorn A.M.;
RT "Molecular components of the endoderm specification pathway in Xenopus
RT tropicalis.";
RL Dev. Dyn. 226:118-127(2003).
CC -!- FUNCTION: Transcription factor required for both mesoderm and endoderm
CC formation in the embryo; signaling determinants and concentration
CC levels may determine which germ layer is formed. Acts together with
CC beta-catenin to activate genes that are responsible for mesoderm
CC induction including wnt-8, eomes t/bra, siamois, mix1 and sox17.
CC Directly binds to promoter DNA. Patterns the mesoderm along the
CC dorsoventral and posterior axis. Activates siamois gene transcription
CC when alone or in combination with beta-catenin, but inhibits siamois
CC transcription in combination with pou5f1.1/oct-25 (By similarity).
CC {ECO:0000250|UniProtKB:P87377}.
CC -!- SUBUNIT: Forms a repression complex on the promoters of the nodal/nr1
CC and siamois genes with the maternal factors tcf7l1/tcf3 and
CC pouf5.1/oct-25. Interacts (via C-terminus) with tcf7l1/tcf3 (via N-
CC terminus). Also interacts with the other POU-domain transcription
CC factors pou5f1.2/oct-91 and pou5f1.3/oct-60 (By similarity).
CC {ECO:0000250|UniProtKB:P87377}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:P87377,
CC ECO:0000255|PROSITE-ProRule:PRU00201}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1 {ECO:0000269|Ref.1}; Synonyms=Maternal VegT
CC {ECO:0000312|EMBL:BAH04511.1}, mVegT {ECO:0000312|EMBL:BAH04511.1};
CC IsoId=Q28HY0-1; Sequence=Displayed;
CC Name=2 {ECO:0000269|Ref.2}; Synonyms=Zygotic VegT
CC {ECO:0000312|EMBL:BAH04512.1}, zVegT {ECO:0000312|EMBL:BAH04512.1};
CC IsoId=Q28HY0-2; Sequence=VSP_053169;
CC -!- TISSUE SPECIFICITY: Vegetally localized in oocytes and expressed in the
CC presumptive endoderm and mesoderm at early gastrula stage. Expression
CC is down-regulated in the endoderm by the end of gastrulation but
CC maintained in the lateral and ventral mesoderm of the blastopore lip.
CC {ECO:0000269|PubMed:12508233}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC {ECO:0000269|PubMed:12508233}.
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DR EMBL; AF180352; AAK00597.1; -; mRNA.
DR EMBL; AB451530; BAH04511.1; -; Genomic_DNA.
DR EMBL; AB451530; BAH04512.1; -; Genomic_DNA.
DR EMBL; CR760687; CAJ82211.1; -; mRNA.
DR EMBL; BC167867; AAI67867.1; -; mRNA.
DR RefSeq; NP_988858.1; NM_203527.1.
DR RefSeq; XP_012813464.1; XM_012958010.1. [Q28HY0-2]
DR AlphaFoldDB; Q28HY0; -.
DR SMR; Q28HY0; -.
DR STRING; 8364.ENSXETP00000009288; -.
DR PaxDb; Q28HY0; -.
DR PRIDE; Q28HY0; -.
DR GeneID; 394452; -.
DR KEGG; xtr:394452; -.
DR CTD; 394452; -.
DR Xenbase; XB-GENE-1033829; vegt.
DR eggNOG; KOG3585; Eukaryota.
DR HOGENOM; CLU_049545_0_0_1; -.
DR InParanoid; Q28HY0; -.
DR OMA; PLFPYAC; -.
DR OrthoDB; 810611at2759; -.
DR TreeFam; TF106341; -.
DR Proteomes; UP000008143; Chromosome 1.
DR Proteomes; UP000790000; Unplaced.
DR Bgee; ENSXETG00000004260; Expressed in blastula and 27 other tissues.
DR ExpressionAtlas; Q28HY0; baseline and differential.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0001708; P:cell fate specification; IBA:GO_Central.
DR GO; GO:0045893; P:positive regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd00182; TBOX; 1.
DR Gene3D; 2.60.40.820; -; 1.
DR InterPro; IPR008967; p53-like_TF_DNA-bd.
DR InterPro; IPR046360; T-box_DNA-bd.
DR InterPro; IPR036960; T-box_sf.
DR InterPro; IPR001699; TF_T-box.
DR InterPro; IPR018186; TF_T-box_CS.
DR PANTHER; PTHR11267; PTHR11267; 2.
DR Pfam; PF00907; T-box; 1.
DR PRINTS; PR00937; TBOX.
DR SMART; SM00425; TBOX; 1.
DR SUPFAM; SSF49417; SSF49417; 1.
DR PROSITE; PS01283; TBOX_1; 1.
DR PROSITE; PS01264; TBOX_2; 1.
DR PROSITE; PS50252; TBOX_3; 1.
PE 2: Evidence at transcript level;
KW Activator; Alternative splicing; Developmental protein; DNA-binding;
KW Nucleus; Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..455
FT /note="T-box protein VegT"
FT /id="PRO_0000390494"
FT DNA_BIND 57..230
FT /note="T-box"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00201"
FT REGION 229..276
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 295..360
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 229..244
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 251..276
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 295..340
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..25
FT /note="MRNCCQEHRLSAGHLEPEISSSCAS -> MHSLP (in isoform 2)"
FT /evidence="ECO:0000303|Ref.2"
FT /id="VSP_053169"
FT CONFLICT 19
FT /note="I -> T (in Ref. 1; AAK00597 and 3; AAI67867)"
FT /evidence="ECO:0000305"
FT CONFLICT 56
FT /note="D -> V (in Ref. 1; AAK00597)"
FT /evidence="ECO:0000305"
FT CONFLICT 78
FT /note="F -> L (in Ref. 1; AAK00597)"
FT /evidence="ECO:0000305"
FT CONFLICT 234
FT /note="S -> R (in Ref. 1; AAK00597)"
FT /evidence="ECO:0000305"
FT CONFLICT 307
FT /note="D -> E (in Ref. 1; AAK00597)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 455 AA; 51869 MW; 4B71E3CACD06BF45 CRC64;
MRNCCQEHRL SAGHLEPEIS SSCASDVKSS PDMDSVSSQD SLYLPNSVGA SLEDQDLWAQ
FHQEGTEMII TKSGRRMFPQ CKIRLFGLHP YAKYMLLVDF VPLDNFRYKW NKNQWEAAGK
AEPHPPCRTY VHPDSPAPGA HWMKDAICFQ KLKLTNNTLD QQGHIILHSM HRYKPRFHVV
QSDDMYNSPW GLVQVFSFPE TEFTAVTAYQ NEKITKLKIN HNPFAKGFRE QERSHKRDDV
LKTLQQSPSK RQKRKKWEDS PEAEISDFPK ATRVKEESIM DPAGVYQNWV SDHEANQGLT
PHSPESDGAN QEQQVPSSSS NFYNRNPYRR SSQHLSSPYD LGEPSSRRLT PDVATVPDSD
PDSLAVLHVI PTQNSTQDRT CGVNFSMEAQ MKQPLRGAMY SPYGAEQWMV PAQGQYRPMG
YTAYPTDLST QGAVAHPHSG MSDWSQYSLF PYSCW
