VEIN_DROME
ID VEIN_DROME Reviewed; 623 AA.
AC Q94918; Q9VRQ3;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 28-FEB-2003, sequence version 2.
DT 03-AUG-2022, entry version 180.
DE RecName: Full=Protein vein;
DE AltName: Full=Epidermal growth factor-like protein;
DE AltName: Full=Protein defective dorsal discs;
DE Flags: Precursor;
GN Name=vn; Synonyms=ddd; ORFNames=CG10491;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Embryo, and Imaginal disk;
RX PubMed=8824589; DOI=10.1101/gad.10.18.2302;
RA Schnepp B.C., Grumbling G.B., Donaldson T.D., Simcox A.A.;
RT "Vein is a novel component in the Drosophila epidermal growth factor
RT receptor pathway with similarity to the neuregulins.";
RL Genes Dev. 10:2302-2313(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
CC -!- FUNCTION: Ligand for the EGF receptor. Seems to play a role in the
CC global proliferation of wing disc cells and the larval patterning.
CC Shows a strong synergistic genetic interaction with spi, suggesting a
CC molecular interdependence. Required for the development of interveins
CC cells.
CC -!- INTERACTION:
CC Q94918; A8JUV7: boi; NbExp=3; IntAct=EBI-869384, EBI-6895641;
CC Q94918; Q9VM64: ihog; NbExp=2; IntAct=EBI-869384, EBI-94134;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q94918-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q94918-2; Sequence=VSP_001419;
CC -!- DEVELOPMENTAL STAGE: Expressed in blastoderm embryos in two
CC ventrolateral stripes that are brought to the midline as gastrulation
CC proceeds. In the germ-band retraction stage, expression is seen in the
CC CNS and epidermis. At late blastoderm, expression is localized in the
CC anlagen of the amnioserosa. Expression in the head, cypeolabrum,
CC maxillary and labial lobes, and around the stomodeum throughout embryo
CC development. In late embryos, expression decays in all ectodermal cells
CC and appears in the segmental muscles and the gut wall. In the larva,
CC expression occurs in the dorsal metathoracic disc, the eye-antennal
CC disc and the ventral thoracic disc. Found in the intervein in the pupa.
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DR EMBL; U67935; AAC47293.1; -; mRNA.
DR EMBL; AE014296; AAF50739.2; -; Genomic_DNA.
DR RefSeq; NP_523942.2; NM_079218.3. [Q94918-1]
DR AlphaFoldDB; Q94918; -.
DR SMR; Q94918; -.
DR BioGRID; 64117; 34.
DR IntAct; Q94918; 3.
DR STRING; 7227.FBpp0076790; -.
DR GlyGen; Q94918; 9 sites.
DR PaxDb; Q94918; -.
DR EnsemblMetazoa; FBtr0077082; FBpp0076790; FBgn0003984. [Q94918-1]
DR GeneID; 38657; -.
DR KEGG; dme:Dmel_CG10491; -.
DR CTD; 38657; -.
DR FlyBase; FBgn0003984; vn.
DR VEuPathDB; VectorBase:FBgn0003984; -.
DR eggNOG; ENOG502QRNM; Eukaryota.
DR HOGENOM; CLU_034587_0_0_1; -.
DR InParanoid; Q94918; -.
DR PhylomeDB; Q94918; -.
DR SignaLink; Q94918; -.
DR BioGRID-ORCS; 38657; 0 hits in 1 CRISPR screen.
DR ChiTaRS; vn; fly.
DR GenomeRNAi; 38657; -.
DR PRO; PR:Q94918; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0003984; Expressed in extraembryonic structure and 52 other tissues.
DR ExpressionAtlas; Q94918; baseline and differential.
DR Genevisible; Q94918; DM.
DR GO; GO:0005576; C:extracellular region; NAS:UniProtKB.
DR GO; GO:0005615; C:extracellular space; IDA:FlyBase.
DR GO; GO:0005154; F:epidermal growth factor receptor binding; IDA:FlyBase.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0008201; F:heparin binding; IDA:FlyBase.
DR GO; GO:0048018; F:receptor ligand activity; IDA:FlyBase.
DR GO; GO:0005102; F:signaling receptor binding; IBA:GO_Central.
DR GO; GO:0048513; P:animal organ development; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; IMP:FlyBase.
DR GO; GO:0030031; P:cell projection assembly; IMP:FlyBase.
DR GO; GO:0007173; P:epidermal growth factor receptor signaling pathway; IDA:FlyBase.
DR GO; GO:0007482; P:haltere development; IMP:FlyBase.
DR GO; GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:FlyBase.
DR GO; GO:0008586; P:imaginal disc-derived wing vein morphogenesis; IMP:FlyBase.
DR GO; GO:0035556; P:intracellular signal transduction; IBA:GO_Central.
DR GO; GO:0007479; P:leg disc proximal/distal pattern formation; IEP:FlyBase.
DR GO; GO:0007494; P:midgut development; IMP:FlyBase.
DR GO; GO:0035310; P:notum cell fate specification; IMP:FlyBase.
DR GO; GO:0007477; P:notum development; IMP:FlyBase.
DR GO; GO:0008355; P:olfactory learning; IMP:FlyBase.
DR GO; GO:0007424; P:open tracheal system development; IMP:FlyBase.
DR GO; GO:0007422; P:peripheral nervous system development; IMP:FlyBase.
DR GO; GO:1903688; P:positive regulation of border follicle cell migration; IMP:FlyBase.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:FlyBase.
DR GO; GO:0070374; P:positive regulation of ERK1 and ERK2 cascade; IDA:FlyBase.
DR GO; GO:2000736; P:regulation of stem cell differentiation; IMP:FlyBase.
DR Gene3D; 2.60.40.10; -; 1.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR013098; Ig_I-set.
DR InterPro; IPR003598; Ig_sub2.
DR InterPro; IPR040180; Neuregulin.
DR PANTHER; PTHR11100; PTHR11100; 1.
DR Pfam; PF07679; I-set; 1.
DR SMART; SM00408; IGc2; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR PROSITE; PS00022; EGF_1; 1.
DR PROSITE; PS50026; EGF_3; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; Disulfide bond;
KW EGF-like domain; Glycoprotein; Growth factor; Immunoglobulin domain;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..40
FT /evidence="ECO:0000255"
FT CHAIN 41..623
FT /note="Protein vein"
FT /id="PRO_0000007774"
FT DOMAIN 457..542
FT /note="Ig-like C2-type"
FT DOMAIN 561..599
FT /note="EGF-like"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00076"
FT REGION 70..98
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 130..162
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 184..214
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 229..317
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 131..162
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 189..214
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 229..243
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 244..263
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 264..289
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 300..316
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 76
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 211
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 252
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 350
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 381
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 424
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 449
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 521
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 574
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 478..531
FT /evidence="ECO:0000250"
FT DISULFID 566..577
FT /evidence="ECO:0000250"
FT DISULFID 571..588
FT /evidence="ECO:0000250"
FT DISULFID 590..599
FT /evidence="ECO:0000250"
FT VAR_SEQ 609..623
FT /note="FVAIYGQIHTLNNDY -> SSPESCKNYQGGYY (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:8824589"
FT /id="VSP_001419"
FT CONFLICT 149
FT /note="Missing (in Ref. 1; AAC47293)"
FT /evidence="ECO:0000305"
FT CONFLICT 220
FT /note="S -> T (in Ref. 1; AAC47293)"
FT /evidence="ECO:0000305"
FT CONFLICT 494
FT /note="E -> D (in Ref. 1; AAC47293)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 623 AA; 71698 MW; AFD2724D5C1F56C8 CRC64;
MYAQHLRKWS LKTKKQLMPL ILLIISYMLL LNTCVLSSSA TTQQQQQQQQ QQQHLPRLWE
GSAEESSYYI PLSSDNGSGS SESSAESGSS SSRSSSNNID NNILSRLLSL NSNSLSSRSN
VKLKPATVFD AGSSTPAQQE QHVAAVPEQQ QQQQQQQQSM QKVPNTLINS QIYNLLYNGM
PSEAASSKMR RHIQPSQLPH QPESRAQLPS NYSSRPAVRS YLIESYEMPE SMLEDRSPEQ
AARSRRDGSN TNGSRQQQRT GHRQQLQQDK RDHRRQRQDQ QKEQRQQQQQ RQHKSGNKHQ
QQQQQRRKHQ RKHQRYNRYC SARDPAQLAF AAPTVFQGVF KSMSADRRVN FSATMKVEKV
YKQQHDLQLP TLVRLQFALS NSSGECDIYR ERLMPRGMLR SGNDLQQASD ISYMMFVQQT
NPGNFTILGQ PMRVTHLVVE AVETAVSENY TQNAEVTKIF SKPSKAIIKH GKKLRIVCEV
SGQPPPKVTW FKDEKSINRK RNIYQFKHHK RRSELIVRSF NSSSDAGRYE CRAKNKASKA
IAKRRIMIKA SPVHFPTDRS ASGIPCNFDY CFHNGTCRMI PDINEVYCRC PTEYFGNRCE
NKWPDSRYFV AIYGQIHTLN NDY
