VEL1_YEAST
ID VEL1_YEAST Reviewed; 206 AA.
AC P53058; D6VV77;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Protein VEL1;
DE AltName: Full=Velum formation protein 1;
DE Flags: Precursor;
GN Name=VEL1; OrderedLocusNames=YGL258W; ORFNames=NRA206;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 96604 / S288c / FY1679;
RX PubMed=8972578;
RX DOI=10.1002/(sici)1097-0061(199612)12:15<1555::aid-yea43>3.0.co;2-q;
RA Coissac E., Maillier E., Robineau S., Netter P.;
RT "Sequence of a 39,411 bp DNA fragment covering the left end of chromosome
RT VII of Saccharomyces cerevisiae.";
RL Yeast 12:1555-1562(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169869;
RA Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J.,
RA Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M.,
RA Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L.,
RA Coblenz A., Coglievina M., Coissac E., Defoor E., Del Bino S., Delius H.,
RA Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P.,
RA Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M.,
RA Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A.,
RA Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K.,
RA Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P.,
RA Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E.,
RA Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K.,
RA Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A.,
RA Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S.,
RA Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M.,
RA Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C.,
RA Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M.,
RA Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M.,
RA Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y.,
RA Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L.,
RA Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D.,
RA Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F.,
RA Zaccaria P., Zimmermann M., Zollner A., Kleine K.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome VII.";
RL Nature 387:81-84(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP INDUCTION.
RX PubMed=12788058; DOI=10.1016/s0006-291x(03)00907-0;
RA Ohkuni K., Shirahige K., Kikuchi A.;
RT "Genome-wide expression analysis of NAP1 in Saccharomyces cerevisiae.";
RL Biochem. Biophys. Res. Commun. 306:5-9(2003).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=11935221; DOI=10.1007/s00294-001-0264-9;
RA Terashima H., Fukuchi S., Nakai K., Arisawa M., Hamada K., Yabuki N.,
RA Kitada K.;
RT "Sequence-based approach for identification of cell wall proteins in
RT Saccharomyces cerevisiae.";
RL Curr. Genet. 40:311-316(2002).
RN [6]
RP INDUCTION BY ZINC DEPLETION.
RX PubMed=14660410; DOI=10.1128/aem.69.12.7535-7540.2003;
RA Higgins V.J., Rogers P.J., Dawes I.W.;
RT "Application of genome-wide expression analysis to identify molecular
RT markers useful in monitoring industrial fermentations.";
RL Appl. Environ. Microbiol. 69:7535-7540(2003).
RN [7]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS].
RX PubMed=19756047; DOI=10.1038/msb.2009.64;
RA Kung L.A., Tao S.-C., Qian J., Smith M.G., Snyder M., Zhu H.;
RT "Global analysis of the glycoproteome in Saccharomyces cerevisiae reveals
RT new roles for protein glycosylation in eukaryotes.";
RL Mol. Syst. Biol. 5:308-308(2009).
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000250}.
CC Note=Intracellular soluble fraction. {ECO:0000250}.
CC -!- INDUCTION: In zinc-depleted conditions and has increased expression in
CC NAP1 deletion mutants. {ECO:0000269|PubMed:12788058,
CC ECO:0000269|PubMed:14660410}.
CC -!- PTM: N-glycosylated. {ECO:0000269|PubMed:19756047}.
CC -!- SIMILARITY: Belongs to the VEL1 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X94357; CAA64129.1; -; Genomic_DNA.
DR EMBL; Z72780; CAA96978.1; -; Genomic_DNA.
DR EMBL; BK006941; DAA07861.1; -; Genomic_DNA.
DR PIR; S61603; S61603.
DR RefSeq; NP_011256.1; NM_001181124.1.
DR AlphaFoldDB; P53058; -.
DR BioGRID; 33021; 35.
DR DIP; DIP-4876N; -.
DR IntAct; P53058; 1.
DR STRING; 4932.YGL258W; -.
DR PaxDb; P53058; -.
DR EnsemblFungi; YGL258W_mRNA; YGL258W; YGL258W.
DR GeneID; 852634; -.
DR KEGG; sce:YGL258W; -.
DR SGD; S000003227; VEL1.
DR VEuPathDB; FungiDB:YGL258W; -.
DR eggNOG; ENOG502RZUS; Eukaryota.
DR GeneTree; ENSGT00940000176668; -.
DR HOGENOM; CLU_1349595_0_0_1; -.
DR InParanoid; P53058; -.
DR OMA; GLHGPHC; -.
DR BioCyc; YEAST:G3O-30726-MON; -.
DR PRO; PR:P53058; -.
DR Proteomes; UP000002311; Chromosome VII.
DR RNAct; P53058; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005829; C:cytosol; IDA:SGD.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR InterPro; IPR019435; Vel1-like.
DR Pfam; PF10339; Vel1p; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Glycoprotein; Reference proteome; Signal; Zinc.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..206
FT /note="Protein VEL1"
FT /id="PRO_0000202704"
FT CARBOHYD 26
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 48
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 91
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 139
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 152
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 206 AA; 22152 MW; D4DB805E578CCA2B CRC64;
MSFLSIFTFF SVLISVATTV RFDLTNVTCK GLHGPHCGTY VMEVVGQNGT FLGQSTFVGA
DVLTESAGDA WARYLGQETR FLPKLTTIAS NETKNFSPLI FTTNINTCNP QSIGDAMVPF
ANTVTGEIEY NSWADTADNA SFITGLANQL FNSTDYGVQV ASCYPNFASV ILSTPAVNIF
GKDDTLPDYC TAIQLKAVCP PEAGFD
