VELB_COCH5
ID VELB_COCH5 Reviewed; 357 AA.
AC M2TF54;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2013, sequence version 1.
DT 25-MAY-2022, entry version 27.
DE RecName: Full=Velvet complex subunit 2 {ECO:0000305};
GN Name=VEL2 {ECO:0000303|PubMed:25080135}; ORFNames=COCHEDRAFT_33742;
OS Cochliobolus heterostrophus (strain C5 / ATCC 48332 / race O) (Southern
OS corn leaf blight fungus) (Bipolaris maydis).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Dothideomycetes;
OC Pleosporomycetidae; Pleosporales; Pleosporineae; Pleosporaceae; Bipolaris.
OX NCBI_TaxID=701091;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C5 / ATCC 48332 / race O;
RX PubMed=23236275; DOI=10.1371/journal.ppat.1003037;
RA Ohm R.A., Feau N., Henrissat B., Schoch C.L., Horwitz B.A., Barry K.W.,
RA Condon B.J., Copeland A.C., Dhillon B., Glaser F., Hesse C.N., Kosti I.,
RA LaButti K., Lindquist E.A., Lucas S., Salamov A.A., Bradshaw R.E.,
RA Ciuffetti L., Hamelin R.C., Kema G.H.J., Lawrence C., Scott J.A.,
RA Spatafora J.W., Turgeon B.G., de Wit P.J.G.M., Zhong S., Goodwin S.B.,
RA Grigoriev I.V.;
RT "Diverse lifestyles and strategies of plant pathogenesis encoded in the
RT genomes of eighteen Dothideomycetes fungi.";
RL PLoS Pathog. 8:E1003037-E1003037(2012).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C5 / ATCC 48332 / race O;
RX PubMed=23357949; DOI=10.1371/journal.pgen.1003233;
RA Condon B.J., Leng Y., Wu D., Bushley K.E., Ohm R.A., Otillar R., Martin J.,
RA Schackwitz W., Grimwood J., MohdZainudin N., Xue C., Wang R., Manning V.A.,
RA Dhillon B., Tu Z.J., Steffenson B.J., Salamov A., Sun H., Lowry S.,
RA LaButti K., Han J., Copeland A., Lindquist E., Barry K., Schmutz J.,
RA Baker S.E., Ciuffetti L.M., Grigoriev I.V., Zhong S., Turgeon B.G.;
RT "Comparative genome structure, secondary metabolite, and effector coding
RT capacity across Cochliobolus pathogens.";
RL PLoS Genet. 9:E1003233-E1003233(2013).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=25080135; DOI=10.1016/j.fgb.2014.07.010;
RA Wang W., Wu D., Pan H., Turgeon B.G.;
RT "Vel2 and Vos1 hold essential roles in ascospore and asexual spore
RT development of the heterothallic maize pathogen Cochliobolus
RT heterostrophus.";
RL Fungal Genet. Biol. 70:113-124(2014).
CC -!- FUNCTION: Component of the velvet transcription factor complex that
CC controls sexual/asexual developmental ratio in response to light,
CC promoting sexual development in the darkness while stimulating asexual
CC sporulation under illumination (PubMed:25080135). The velvet complex
CC acts as a global regulator for secondary metabolite gene expression (By
CC similarity). Component of the VEL2-VOS1 heterodimeric complex that
CC plays a dual role in activating genes associated with spore maturation
CC and repressing certain development-associated genes (By similarity).
CC The complex binds DNA through the DNA-binding domain of VOS1 that
CC recognizes an 11-nucleotide consensus sequence 5'-CTGGCCGCGGC-3'
CC consisting of two motifs in the promoters of key developmental
CC regulatory genes (By similarity). The VEL2-VOS1 complex is required for
CC normal pseudothecium development and regulates asexual spore
CC compartmentalization, pigmentation and germination (PubMed:25080135).
CC {ECO:0000250|UniProtKB:C8VTS4, ECO:0000269|PubMed:25080135}.
CC -!- SUBUNIT: Component of the heterotrimeric velvet complex composed of
CC LAE1, VEL1 and VEL2; VEL1A acting as a bridging protein between LAE1
CC and VEL2 (By similarity). Forms a heterodimeric complex with VOS1; the
CC formation of the VEL2-VOS1 complex is light-dependent (By similarity).
CC {ECO:0000250|UniProtKB:C8VTS4}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C8VTS4}. Cytoplasm
CC {ECO:0000250|UniProtKB:C8VTS4}. Note=Nuclear localization is mediated
CC by VEL1 (By similarity). {ECO:0000250|UniProtKB:C8VTS4}.
CC -!- DISRUPTION PHENOTYPE: Produces only albino pseudothecia
CC (PubMed:25080135). reduces tolerance to oxidative and thermal stresses
CC (PubMed:25080135). Leads to increased expression of VOS1
CC (PubMed:25080135). {ECO:0000269|PubMed:25080135}.
CC -!- SIMILARITY: Belongs to the velvet family. VelB subfamily.
CC {ECO:0000305}.
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DR EMBL; KB445593; EMD85134.1; -; Genomic_DNA.
DR AlphaFoldDB; M2TF54; -.
DR SMR; M2TF54; -.
DR STRING; 701091.M2TF54; -.
DR EnsemblFungi; EMD85134; EMD85134; COCHEDRAFT_33742.
DR eggNOG; ENOG502S1B4; Eukaryota.
DR HOGENOM; CLU_022491_0_0_1; -.
DR OMA; KIGVWFI; -.
DR Proteomes; UP000016936; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.3960; -; 2.
DR InterPro; IPR021740; Velvet.
DR InterPro; IPR037525; Velvet_dom.
DR InterPro; IPR038491; Velvet_dom_sf.
DR PANTHER; PTHR33572; PTHR33572; 1.
DR Pfam; PF11754; Velvet; 1.
DR PROSITE; PS51821; VELVET; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Nucleus; Reference proteome; Sporulation; Transcription;
KW Transcription regulation.
FT CHAIN 1..357
FT /note="Velvet complex subunit 2"
FT /id="PRO_0000435784"
FT DOMAIN 32..341
FT /note="Velvet"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01165"
SQ SEQUENCE 357 AA; 39179 MW; 34F1026AEFB490B4 CRC64;
MAASMAHYQG HLSNRITTGR AETVGQAQYP PRRAERKYKL EVEQQPIRAR MCGFGDKDRR
PITPPPCIRL VVLDEMDREL DFNEIDSTYF VLMVDLWNES GQSAVNLVRH SSAAPTVSIS
SSTTTSYPPP PERSHYVATT IPGYDAHSQA YRHQQHQHQH QHQHMQPQPM AAAGYGPGAH
TGVAVPPYYP PTPQPPTPTY QQYPAPAPAA PYGQAPAPSA MIPATPMSSN HTRNLIGMNA
VNACRLNDTK NKPGFWFVLQ DLSVRTEGTF RLKLFLFDIG AGDGTNATVA ADGPTRGKGK
CLAVNFSDPF TVYSAKKFPG VIESTPLSKC FAQQGIKIPI RKDGPKLPNQ AEYDADD
