VELB_FUSO4
ID VELB_FUSO4 Reviewed; 481 AA.
AC A0A0J9U3L6; A0A0D2X810; A0A0J9U609;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 14-OCT-2015, sequence version 1.
DT 25-MAY-2022, entry version 24.
DE RecName: Full=Velvet complex subunit B {ECO:0000305};
GN Name=velB {ECO:0000303|PubMed:23106229}; ORFNames=FOXG_00016;
OS Fusarium oxysporum f. sp. lycopersici (strain 4287 / CBS 123668 / FGSC 9935
OS / NRRL 34936) (Fusarium vascular wilt of tomato).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium oxysporum species complex.
OX NCBI_TaxID=426428;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=4287 / CBS 123668 / FGSC 9935 / NRRL 34936;
RX PubMed=20237561; DOI=10.1038/nature08850;
RA Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT "Comparative genomics reveals mobile pathogenicity chromosomes in
RT Fusarium.";
RL Nature 464:367-373(2010).
RN [2]
RP IDENTIFICATION.
RC STRAIN=4287 / CBS 123668 / FGSC 9935 / NRRL 34936;
RG EnsemblFungi;
RL Submitted (MAR-2015) to UniProtKB.
RN [3]
RP DISRUPTION PHENOPTYPE.
RX PubMed=19161356; DOI=10.1111/j.1364-3703.2008.00512.x;
RA Lopez-Berges M.S., di Pietro A., Daboussi M.J., Wahab H.A., Vasnier C.,
RA Roncero M.I., Dufresne M., Hera C.;
RT "Identification of virulence genes in Fusarium oxysporum f. sp. lycopersici
RT by large-scale transposon tagging.";
RL Mol. Plant Pathol. 10:95-107(2009).
RN [4]
RP IDENTIFICATION IN THE VELVET COMPLEX, FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=23106229; DOI=10.1111/mmi.12082;
RA Lopez-Berges M.S., Hera C., Sulyok M., Schaefer K., Capilla J., Guarro J.,
RA Di Pietro A.;
RT "The velvet complex governs mycotoxin production and virulence of Fusarium
RT oxysporum on plant and mammalian hosts.";
RL Mol. Microbiol. 87:49-65(2013).
CC -!- FUNCTION: Component of the velvet transcription factor complex that
CC controls sexual/asexual developmental ratio in response to light,
CC promoting sexual development in the darkness while stimulating asexual
CC sporulation under illumination (By similarity). The velvet complex acts
CC as a global regulator for secondary metabolite gene expression (By
CC similarity). Component of the velB-VosA heterodimeric complex that
CC plays a dual role in activating genes associated with spore maturation
CC and repressing certain development-associated genes (By similarity).
CC The velB-VosA complex binds DNA through the DNA-binding domain of vosA
CC that recognizes an 11-nucleotide consensus sequence 5'-CTGGCCGCGGC-3'
CC consisting of two motifs in the promoters of key developmental
CC regulatory genes (By similarity). Controls the biosynthetic gene
CC cluster for beauvericin, a depsipeptide mycotoxin that functions as a
CC virulence determinant (PubMed:23106229). Regulates also chromatin
CC structure and transcription of siderophore biosynthetic genes and is
CC required for infection of tomato plants (PubMed:23106229).
CC {ECO:0000250|UniProtKB:C8VTS4, ECO:0000269|PubMed:23106229}.
CC -!- SUBUNIT: Component of the heterotrimeric velvet complex composed of
CC laeA, veA and velB; VeA acting as a bridging protein between laeA and
CC velB (PubMed:23106229). Forms a heterodimeric complex with vosA; the
CC formation of the velB-vosA complex is light-dependent (By similarity).
CC {ECO:0000250|UniProtKB:C8VTS4, ECO:0000269|PubMed:23106229}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C8VTS4}. Cytoplasm
CC {ECO:0000250|UniProtKB:C8VTS4}. Note=Nuclear localization is mediated
CC by veA (By similarity). {ECO:0000250|UniProtKB:C8VTS4}.
CC -!- DISRUPTION PHENOTYPE: Impairs production of beauvericin and attenuates
CC virulence during infection of tomato plants (PubMed:19161356,
CC PubMed:23106229). {ECO:0000269|PubMed:19161356,
CC ECO:0000269|PubMed:23106229}.
CC -!- SIMILARITY: Belongs to the velvet family. VelB subfamily.
CC {ECO:0000305}.
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DR EMBL; DS231696; KNA93509.1; -; Genomic_DNA.
DR EMBL; DS231696; KNA93510.1; -; Genomic_DNA.
DR RefSeq; XP_018231555.1; XM_018376051.1.
DR RefSeq; XP_018231556.1; XM_018376052.1.
DR AlphaFoldDB; A0A0J9U3L6; -.
DR SMR; A0A0J9U3L6; -.
DR STRING; 426428.A0A0J9U3L6; -.
DR EnsemblFungi; KNA93509; KNA93509; FOXG_00016.
DR EnsemblFungi; KNA93510; KNA93510; FOXG_00016.
DR GeneID; 28942350; -.
DR KEGG; fox:FOXG_00016; -.
DR VEuPathDB; FungiDB:FOXG_00016; -.
DR Proteomes; UP000009097; Unplaced.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.3960; -; 2.
DR InterPro; IPR021740; Velvet.
DR InterPro; IPR037525; Velvet_dom.
DR InterPro; IPR038491; Velvet_dom_sf.
DR PANTHER; PTHR33572; PTHR33572; 1.
DR Pfam; PF11754; Velvet; 1.
DR PROSITE; PS51821; VELVET; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Sporulation; Transcription;
KW Transcription regulation; Virulence.
FT CHAIN 1..481
FT /note="Velvet complex subunit B"
FT /id="PRO_0000435780"
FT DOMAIN 160..464
FT /note="Velvet"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01165"
FT REGION 1..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 241..339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..18
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 19..77
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 92..139
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 241..303
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 481 AA; 52750 MW; 70142D34E05543F2 CRC64;
MNSAYHSPDM SQRIPGPAYS SSAPPPPIHT YQQHQHPPPP LPPPSQHHHS SHPPLPPPPS
APHPHHQHPP PPPPSHSLSS HQHHSQPPHH QSQLPPYAPA PYQQPQPSQY PRPHPHQQHA
PSPSQHDEPP PPPPFSGPSP TDHQKDSEYV PPSYSKIEEG SGWKYSLDVK QQPVRARMCG
FGDKDRRPIT PPPCVRLVII NTETGKEVDY NSLDHAMFVL SVDLWNHDGT KEVNLVRSST
GTGAMASSST YTYSSLEPGT PSYQQQSLPP SRESGYGQSQ GLNYGQDYPP PVQQSYGQAP
SYPPSSSYGP PQQYYPRHSG YSAEPSAPPP GAPFRNGYGQ DQNALTRMAV VGGQPQGMFT
RNLIGSLAAS AFRLEDTEGQ SGIWFVLQDL SVRTEGTFRL RFSFVNVGRP GGQGTNVNQG
RAPILSSCYS ESFHVYSAKK FPGVCESTPL SKKFANQGIK IPIRKDANIK GEGDEEMYDQ
N
