VELB_GIBM7
ID VELB_GIBM7 Reviewed; 448 AA.
AC W7LFK5;
DT 16-MAR-2016, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 25.
DE RecName: Full=Velvet complex subunit 2 {ECO:0000305};
GN Name=VELB {ECO:0000303|PubMed:24792348}; ORFNames=FVEG_01498;
OS Gibberella moniliformis (strain M3125 / FGSC 7600) (Maize ear and stalk rot
OS fungus) (Fusarium verticillioides).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium;
OC Fusarium fujikuroi species complex.
OX NCBI_TaxID=334819;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=M3125 / FGSC 7600;
RX PubMed=20237561; DOI=10.1038/nature08850;
RA Ma L.-J., van der Does H.C., Borkovich K.A., Coleman J.J., Daboussi M.-J.,
RA Di Pietro A., Dufresne M., Freitag M., Grabherr M., Henrissat B.,
RA Houterman P.M., Kang S., Shim W.-B., Woloshuk C., Xie X., Xu J.-R.,
RA Antoniw J., Baker S.E., Bluhm B.H., Breakspear A., Brown D.W.,
RA Butchko R.A.E., Chapman S., Coulson R., Coutinho P.M., Danchin E.G.J.,
RA Diener A., Gale L.R., Gardiner D.M., Goff S., Hammond-Kosack K.E.,
RA Hilburn K., Hua-Van A., Jonkers W., Kazan K., Kodira C.D., Koehrsen M.,
RA Kumar L., Lee Y.-H., Li L., Manners J.M., Miranda-Saavedra D.,
RA Mukherjee M., Park G., Park J., Park S.-Y., Proctor R.H., Regev A.,
RA Ruiz-Roldan M.C., Sain D., Sakthikumar S., Sykes S., Schwartz D.C.,
RA Turgeon B.G., Wapinski I., Yoder O., Young S., Zeng Q., Zhou S.,
RA Galagan J., Cuomo C.A., Kistler H.C., Rep M.;
RT "Comparative genomics reveals mobile pathogenicity chromosomes in
RT Fusarium.";
RL Nature 464:367-373(2010).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH VE1, FUNCTION, AND
RP DISRUPTION PHENOTYPE.
RX PubMed=24792348; DOI=10.1128/ec.00022-14;
RA Lan N., Zhang H., Hu C., Wang W., Calvo A.M., Harris S.D., Chen S., Li S.;
RT "Coordinated and distinct functions of velvet proteins in Fusarium
RT verticillioides.";
RL Eukaryot. Cell 13:909-918(2014).
CC -!- FUNCTION: Component of the velvet transcription factor complex that
CC controls sexual/asexual developmental ratio in response to light,
CC promoting sexual development in the darkness while stimulating asexual
CC sporulation under illumination (PubMed:24792348). The velvet complex
CC acts as a global regulator for secondary metabolite gene expression (By
CC similarity). Component of the VELB-VOS1 heterodimeric complex that
CC plays a dual role in activating genes associated with spore maturation
CC and repressing certain development-associated genes (By similarity).
CC The VELB-VOS1 complex binds DNA through the DNA-binding domain of VOS1
CC that recognizes an 11-nucleotide consensus sequence 5'-CTGGCCGCGGC-3'
CC consisting of two motifs in the promoters of key developmental
CC regulatory genes (By similarity). Controls the expression of the
CC fumonisins gene cluster (PubMed:24792348). Involved in cell wall
CC integrity, cell surface hydrophobicity, hyphal polarity and conidiation
CC pattern (PubMed:24792348). Involved in oxidative stress resistance by
CC positively regulating the transcription of the catalase-encoding gene
CC CAT2 (PubMed:24792348). {ECO:0000250|UniProtKB:C8VTS4,
CC ECO:0000269|PubMed:24792348}.
CC -!- SUBUNIT: Component of the heterotrimeric velvet complex composed of
CC LAE1, VE1 and VELB; VE1 acting as a bridging protein between LAE1 and
CC VEL2 (By similarity). Interacts with VE1 (PubMed:24792348). Forms a
CC heterodimeric complex with VOS1; the formation of the VELB-VOS1 complex
CC is light-dependent (By similarity). {ECO:0000250|UniProtKB:C8VTS4,
CC ECO:0000269|PubMed:24792348}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:C8VTS4}. Cytoplasm
CC {ECO:0000250|UniProtKB:C8VTS4}. Note=Nuclear localization is mediated
CC by VE1 (By similarity). {ECO:0000250|UniProtKB:C8VTS4}.
CC -!- DISRUPTION PHENOTYPE: Suppresses aerial hyphal growth,reduced colony
CC surface hydrophobicity on solid media, and increases the ratio of
CC macroconidia to microconidia (PubMed:24792348). Reduces the production
CC of fumonisins (PubMed:24792348). {ECO:0000269|PubMed:24792348}.
CC -!- SIMILARITY: Belongs to the velvet family. VelB subfamily.
CC {ECO:0000305}.
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DR EMBL; DS022242; EWG38233.1; -; Genomic_DNA.
DR RefSeq; XP_018744424.1; XM_018888508.1.
DR AlphaFoldDB; W7LFK5; -.
DR SMR; W7LFK5; -.
DR STRING; 117187.FVEG_01498T0; -.
DR GeneID; 30059790; -.
DR KEGG; fvr:FVEG_01498; -.
DR VEuPathDB; FungiDB:FVEG_01498; -.
DR eggNOG; ENOG502S1B4; Eukaryota.
DR OrthoDB; 1407766at2759; -.
DR Proteomes; UP000009096; Chromosome 1.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 2.60.40.3960; -; 1.
DR InterPro; IPR021740; Velvet.
DR InterPro; IPR037525; Velvet_dom.
DR InterPro; IPR038491; Velvet_dom_sf.
DR PANTHER; PTHR33572; PTHR33572; 1.
DR Pfam; PF11754; Velvet; 1.
DR PROSITE; PS51821; VELVET; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Sporulation; Transcription;
KW Transcription regulation.
FT CHAIN 1..448
FT /note="Velvet complex subunit 2"
FT /id="PRO_0000435782"
FT DOMAIN 155..431
FT /note="Velvet"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01165"
FT REGION 1..153
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 224..306
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 30..75
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 110..134
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 225..257
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 260..277
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 448 AA; 49370 MW; 2F850461E0B7F0A1 CRC64;
MNSAYHPPDM SQRIPGPAYS SSAPPPIHTY QQHQHPPPPL PPPSQHHHSS HPPLPPPPSA
PHPHHQHPPP PPSHSLSSHQ HHGQPPHHQP QPYAPAPYQQ SQPSQYPRPH PHQQHVPPPS
QHDEPPPPPS SGPSPTDHQK DSEYVPPSYS KIEEGSGWKY SLDVKQQPVR ARMCGFGDKD
RRPITPPPCV RLVIINTETG KEVDYNTLDH AMFVLSVDLW NHDEPGTPSY QQQSLPPSRE
SGYGQSQGMN YGQDYPPPVQ QSYGPAPSYP PSSSYGPPQQ YYPRHSGYSA EPSAPPPGAP
FRNGYGQDQN ALTRMAVVGG QPQGMFTRNL IGSLAASAFR LEDTEGQSGI WFVLQDLSVR
TEGTFRLRFS FVNVGRPGGQ GTNVNQGRAP ILSSCYSESF HVYSAKKFPG VCESTPLSKK
FANQGIKIPI RKDANIKGEG DEEMYDQN
