ID   VEMP_CVHNL              Reviewed;          77 AA.
AC   Q6Q1S0;
DT   17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   02-JUN-2021, entry version 68.
DE   RecName: Full=Envelope small membrane protein {ECO:0000255|HAMAP-Rule:MF_04205};
DE            Short=E protein {ECO:0000255|HAMAP-Rule:MF_04205};
DE            Short=sM protein {ECO:0000255|HAMAP-Rule:MF_04205};
GN   Name=E {ECO:0000255|HAMAP-Rule:MF_04205}; Synonyms=sM; ORFNames=4;
OS   Human coronavirus NL63 (HCoV-NL63).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Setracovirus.
OX   NCBI_TaxID=277944;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Amsterdam I;
RX   PubMed=15034574; DOI=10.1038/nm1024;
RA   Van Der Hoek L., Pyrc K., Jebbink M.F., Vermeulen-Oost W., Berkhout R.J.,
RA   Wolthers K.C., Wertheim-Van Dillen P.M., Kaandorp J., Spaargaren J.,
RA   Berkhout B.;
RT   "Identification of a new human coronavirus.";
RL   Nat. Med. 10:368-373(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate Amsterdam 057, and Isolate Amsterdam 496;
RX   PubMed=17054987; DOI=10.1016/j.jmb.2006.09.074;
RA   Pyrc K., Dijkman R., Deng L., Jebbink M.F., Ross H.A., Berkhout B.,
RA   van der Hoek L.;
RT   "Mosaic structure of human coronavirus NL63, one thousand years of
RT   evolution.";
RL   J. Mol. Biol. 364:964-973(2006).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC   STRAIN=Isolate BJ8081;
RA   Xing J., Zhu R., Qian Y., Deng J., Wang F., Zhao L., Sun Y., Liao B.;
RT   "Sequence analysis of envelope protein gene from Human coronavirus NL63
RT   identified from specimen collected from a child with acute respiratory
RT   infection in Beijing, China.";
RL   Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a central role in virus morphogenesis and assembly.
CC       Acts as a viroporin and self-assembles in host membranes forming
CC       pentameric protein-lipid pores that allow ion transport. Also plays a
CC       role in the induction of apoptosis. {ECO:0000255|HAMAP-Rule:MF_04205}.
CC   -!- SUBUNIT: Homopentamer. Interacts with membrane protein M in the budding
CC       compartment of the host cell, which is located between endoplasmic
CC       reticulum and the Golgi complex. Interacts with Nucleoprotein.
CC       {ECO:0000255|HAMAP-Rule:MF_04205}.
CC   -!- SUBCELLULAR LOCATION: Host Golgi apparatus membrane {ECO:0000255|HAMAP-
CC       Rule:MF_04205}; Single-pass type III membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_04205}. Note=The cytoplasmic tail functions
CC       as a Golgi complex-targeting signal. {ECO:0000255|HAMAP-Rule:MF_04205}.
CC   -!- SIMILARITY: Belongs to the alphacoronaviruses E protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_04205}.
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DR   EMBL; AY567487; AAS58179.1; -; Genomic_RNA.
DR   EMBL; DQ445911; ABE97139.1; -; Genomic_RNA.
DR   EMBL; DQ445912; ABE97132.1; -; Genomic_RNA.
DR   EMBL; DQ834909; ABI24281.1; -; Genomic_RNA.
DR   RefSeq; YP_003769.1; NC_005831.2.
DR   DNASU; 2943502; -.
DR   GeneID; 2943502; -.
DR   KEGG; vg:2943502; -.
DR   Proteomes; UP000008573; Genome.
DR   Proteomes; UP000115484; Genome.
DR   Proteomes; UP000138462; Genome.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0044662; P:disruption by virus of host cell membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0039707; P:pore formation by virus in membrane of host cell; IEA:InterPro.
DR   GO; GO:0046760; P:viral budding from Golgi membrane; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04205; ALPHA_CORONA_E; 1.
DR   InterPro; IPR043507; E_protein_aCoV.
DR   InterPro; IPR003873; E_protein_CoV.
DR   Pfam; PF02723; CoV_E; 1.
DR   PROSITE; PS51926; COV_E; 1.
PE   3: Inferred from homology;
KW   Apoptosis; Host Golgi apparatus; Host membrane; Membrane; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..77
FT                   /note="Envelope small membrane protein"
FT                   /id="PRO_0000283974"
FT   TOPO_DOM        1..15
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04205"
FT   TRANSMEM        16..36
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04205"
FT   TOPO_DOM        37..77
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04205"
SQ   SEQUENCE   77 AA;  9178 MW;  921B5332B22B3687 CRC64;
     MFLRLIDDNG IVLNSILWLL VMIFFFVLAM TFIKLIQLCF TCHYFFSRTL YQPVYKIFLA
     YQDYMQIAPV PAEVLNV