ID   VEMP_CVPPU              Reviewed;          82 AA.
AC   P09048;
DT   01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1988, sequence version 1.
DT   07-OCT-2020, entry version 90.
DE   RecName: Full=Envelope small membrane protein {ECO:0000255|HAMAP-Rule:MF_04205};
DE            Short=E protein {ECO:0000255|HAMAP-Rule:MF_04205};
DE            Short=sM protein {ECO:0000255|HAMAP-Rule:MF_04205};
GN   Name=E {ECO:0000255|HAMAP-Rule:MF_04205}; Synonyms=sM; ORFNames=4;
OS   Porcine transmissible gastroenteritis coronavirus (strain Purdue) (TGEV).
OC   Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC   Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC   Alphacoronavirus; Tegacovirus.
OX   NCBI_TaxID=11151;
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=2825819; DOI=10.1016/0300-9084(87)90178-7;
RA   Rasschaert D., Gelfi J., Laude H.;
RT   "Enteric coronavirus TGEV: partial sequence of the genomic RNA, its
RT   organization and expression.";
RL   Biochimie 69:591-600(1987).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RX   PubMed=10805807; DOI=10.1073/pnas.97.10.5516;
RA   Almazan F., Gonzalez J.M., Penzes Z., Izeta A., Calvo E., Plana-Duran J.,
RA   Enjuanes L.;
RT   "Engineering the largest RNA virus genome as an infectious bacterial
RT   artificial chromosome.";
RL   Proc. Natl. Acad. Sci. U.S.A. 97:5516-5521(2000).
RN   [3]
RP   NUCLEOTIDE SEQUENCE.
RA   Kapke P.A., Tung F.Y.T., Brian D.A.;
RL   Submitted (SEP-1988) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a central role in virus morphogenesis and assembly.
CC       Acts as a viroporin and self-assembles in host membranes forming
CC       pentameric protein-lipid pores that allow ion transport. Also plays a
CC       role in the induction of apoptosis. {ECO:0000255|HAMAP-Rule:MF_04205}.
CC   -!- SUBUNIT: Homopentamer. Interacts with membrane protein M in the budding
CC       compartment of the host cell, which is located between endoplasmic
CC       reticulum and the Golgi complex. Interacts with Nucleoprotein.
CC       {ECO:0000255|HAMAP-Rule:MF_04205}.
CC   -!- SUBCELLULAR LOCATION: Host Golgi apparatus membrane {ECO:0000255|HAMAP-
CC       Rule:MF_04205}; Single-pass type III membrane protein
CC       {ECO:0000255|HAMAP-Rule:MF_04205}. Note=The cytoplasmic tail functions
CC       as a Golgi complex-targeting signal. {ECO:0000255|HAMAP-Rule:MF_04205}.
CC   -!- SIMILARITY: Belongs to the alphacoronaviruses E protein family.
CC       {ECO:0000255|HAMAP-Rule:MF_04205}.
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DR   EMBL; X06371; CAA29672.1; -; Genomic_RNA.
DR   EMBL; AJ271965; CAB91148.1; -; Genomic_RNA.
DR   EMBL; X12800; CAA31288.1; -; Genomic_DNA.
DR   PIR; S01741; S01741.
DR   Proteomes; UP000001440; Genome.
DR   GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0044662; P:disruption by virus of host cell membrane; IEA:UniProtKB-UniRule.
DR   GO; GO:0039707; P:pore formation by virus in membrane of host cell; IEA:InterPro.
DR   GO; GO:0046760; P:viral budding from Golgi membrane; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_04205; ALPHA_CORONA_E; 1.
DR   InterPro; IPR043507; E_protein_aCoV.
DR   InterPro; IPR003873; E_protein_CoV.
DR   Pfam; PF02723; CoV_E; 1.
DR   PROSITE; PS51926; COV_E; 1.
PE   3: Inferred from homology;
KW   Apoptosis; Host Golgi apparatus; Host membrane; Membrane;
KW   Reference proteome; Transmembrane; Transmembrane helix.
FT   CHAIN           1..82
FT                   /note="Envelope small membrane protein"
FT                   /id="PRO_0000106090"
FT   TOPO_DOM        1..19
FT                   /note="Virion surface"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04205"
FT   TRANSMEM        20..40
FT                   /note="Helical"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04205"
FT   TOPO_DOM        41..82
FT                   /note="Intravirion"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_04205"
SQ   SEQUENCE   82 AA;  9239 MW;  43645DA9BC3688F4 CRC64;
     MTFPRALTVI DDNGMVINII FWFLLIIILI LLSIALLNII KLCMVCCNLG RTVIIVPAQH
     AYDAYKNFMR IKAYNPDGAL LA