VEMP_IBVB
ID VEMP_IBVB Reviewed; 108 AA.
AC Q89894; Q4ZJS8;
DT 17-APR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 85.
DE RecName: Full=Envelope small membrane protein {ECO:0000255|HAMAP-Rule:MF_04206};
DE Short=E protein {ECO:0000255|HAMAP-Rule:MF_04206};
DE Short=sM protein {ECO:0000255|HAMAP-Rule:MF_04206};
GN Name=E {ECO:0000255|HAMAP-Rule:MF_04206}; Synonyms=sM; ORFNames=3c;
OS Avian infectious bronchitis virus (strain Beaudette) (IBV).
OC Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes;
OC Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae;
OC Gammacoronavirus; Igacovirus.
OX NCBI_TaxID=11122;
OH NCBI_TaxID=9031; Gallus gallus (Chicken).
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=2995560; DOI=10.1099/0022-1317-66-10-2253;
RA Boursnell M.E.G., Binns M.M., Brown T.D.K.;
RT "Sequencing of coronavirus IBV genomic RNA: three open reading frames in
RT the 5' 'unique' region of mRNA D.";
RL J. Gen. Virol. 66:2253-2258(1985).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX PubMed=3027249; DOI=10.1099/0022-1317-68-1-57;
RA Boursnell M.E.G., Brown T.D.K., Foulds I.J., Green P.F., Tomley F.M.,
RA Binns M.M.;
RT "Completion of the sequence of the genome of the coronavirus avian
RT infectious bronchitis virus.";
RL J. Gen. Virol. 68:57-77(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2829563; DOI=10.1007/978-1-4684-1280-2_6;
RA Smith A.R., Boursnell M.E.G., Binns M.M., Brown T.D.K., Inglis S.C.;
RT "Identification of a new gene product encoded by mRNA D of infectious
RT bronchitis virus.";
RL Adv. Exp. Med. Biol. 218:47-54(1987).
RN [4]
RP NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RC STRAIN=Isolate Vero cell-adapted p65;
RX PubMed=16137658; DOI=10.1016/j.bbrc.2005.08.105;
RA Fang S.G., Shen S., Tay F.P., Liu D.X.;
RT "Selection of and recombination between minor variants lead to the
RT adaptation of an avian coronavirus to primate cells.";
RL Biochem. Biophys. Res. Commun. 336:417-423(2005).
RN [5]
RP INTERACTION WITH M PROTEIN.
RX PubMed=11774531; DOI=10.1007/978-1-4615-1325-4_88;
RA Lim K.P., Xu H.Y., Liu D.X.;
RT "Physical interaction between the membrane (M) and envelope (E) proteins of
RT the coronavirus avian infectious bronchitis virus (IBV).";
RL Adv. Exp. Med. Biol. 494:595-602(2001).
RN [6]
RP TOPOLOGY, AND SUBCELLULAR LOCATION.
RX PubMed=10756047; DOI=10.1128/jvi.74.9.4319-4326.2000;
RA Corse E., Machamer C.E.;
RT "Infectious bronchitis virus E protein is targeted to the Golgi complex and
RT directs release of virus-like particles.";
RL J. Virol. 74:4319-4326(2000).
RN [7]
RP INTERACTION WITH M PROTEIN.
RX PubMed=12890618; DOI=10.1016/s0042-6822(03)00175-2;
RA Corse E., Machamer C.E.;
RT "The cytoplasmic tails of infectious bronchitis virus E and M proteins
RT mediate their interaction.";
RL Virology 312:25-34(2003).
CC -!- FUNCTION: Plays a central role in virus morphogenesis and assembly.
CC Acts as a viroporin and self-assembles in host membranes forming
CC pentameric protein-lipid pores that allow ion transport. Also plays a
CC role in the induction of apoptosis. {ECO:0000255|HAMAP-Rule:MF_04206}.
CC -!- SUBUNIT: Homooligomer. Interacts with the M membrane protein in the
CC budding compartment of the host cell, which is located between
CC endoplasmic reticulum and the Golgi complex. The cytoplasmic tails of
CC both proteins are important for this function. Interacts with
CC Nucleoprotein. {ECO:0000255|HAMAP-Rule:MF_04206,
CC ECO:0000269|PubMed:11774531, ECO:0000269|PubMed:12890618}.
CC -!- SUBCELLULAR LOCATION: Host Golgi apparatus membrane {ECO:0000255|HAMAP-
CC Rule:MF_04206}; Single-pass type III membrane protein
CC {ECO:0000255|HAMAP-Rule:MF_04206}. Note=The cytoplasmic tail functions
CC as a Golgi complex-targeting signal. {ECO:0000255|HAMAP-Rule:MF_04206}.
CC -!- SIMILARITY: Belongs to the gammacoronaviruses E protein family.
CC {ECO:0000255|HAMAP-Rule:MF_04206}.
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DR EMBL; M95169; AAA70238.1; -; Genomic_RNA.
DR EMBL; M27435; AAA46232.1; -; mRNA.
DR EMBL; DQ001339; AAY24436.1; -; Genomic_RNA.
DR RefSeq; NP_040834.1; NC_001451.1.
DR SMR; Q89894; -.
DR TCDB; 1.A.99.1.1; the infectious bronchitis virus envelope small membrane protein e (ibv-e) family.
DR GeneID; 1489742; -.
DR KEGG; vg:1489742; -.
DR Proteomes; UP000006717; Genome.
DR GO; GO:0044178; C:host cell Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0044662; P:disruption by virus of host cell membrane; IEA:UniProtKB-UniRule.
DR GO; GO:0039707; P:pore formation by virus in membrane of host cell; IEA:InterPro.
DR GO; GO:0046760; P:viral budding from Golgi membrane; IDA:UniProtKB.
DR HAMAP; MF_04206; GAMMA_CORONA_E; 1.
DR InterPro; IPR003873; E_protein_CoV.
DR InterPro; IPR005296; IBV_3C.
DR Pfam; PF03620; IBV_3C; 1.
DR PROSITE; PS51926; COV_E; 1.
PE 1: Evidence at protein level;
KW Apoptosis; Host Golgi apparatus; Host membrane; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..108
FT /note="Envelope small membrane protein"
FT /id="PRO_0000283979"
FT TOPO_DOM 1..10
FT /note="Virion surface"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04206"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04206"
FT TOPO_DOM 32..108
FT /note="Intravirion"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_04206"
FT REGION 88..108
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 72
FT /note="L -> I (in strain: Isolate Vero cell-adapted p65)"
FT VARIANT 101
FT /note="A -> V (in strain: Isolate Vero cell-adapted p65)"
SQ SEQUENCE 108 AA; 12248 MW; 6EC433247DBD132C CRC64;
MNLLNKSLEE NGSFLTALYI IVGFLALYLL GRALQAFVQA ADACCLFWYT WVVIPGAKGT
AFVYKYTYGR KLNNPELEAV IVNEFPKNGW NNKNPANFQD AQRDKLYS
