ID   VENB_VIBVU              Reviewed;         295 AA.
AC   P74965;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2003, sequence version 2.
DT   25-MAY-2022, entry version 126.
DE   RecName: Full=Probable isochorismatase;
DE            EC=3.3.2.1;
DE   AltName: Full=2,3 dihydro-2,3 dihydroxybenzoate synthase;
GN   Name=venB; OrderedLocusNames=VV2_0838;
OS   Vibrio vulnificus (strain CMCP6).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=216895;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=MO6-24;
RX   PubMed=8698519; DOI=10.1128/iai.64.7.2834-2838.1996;
RA   Litwin C.M., Rayback T.W., Skinner J.;
RT   "Role of catechol siderophore synthesis in Vibrio vulnificus virulence.";
RL   Infect. Immun. 64:2834-2838(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CMCP6;
RA   Rhee J.H., Kim S.Y., Chung S.S., Kim J.J., Moon Y.H., Jeong H., Choy H.E.;
RT   "Complete genome sequence of Vibrio vulnificus CMCP6.";
RL   Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Involved in the biosynthesis of the catechol siderophore
CC       vulnibactin. Vulnibactin is a chelating compound involved in
CC       transporting iron from the bacterial environment into the cell
CC       cytoplasm.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=H2O + isochorismate = (2S,3S)-2,3-dihydroxy-2,3-
CC         dihydrobenzoate + pyruvate; Xref=Rhea:RHEA:11112, ChEBI:CHEBI:15361,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:29780, ChEBI:CHEBI:58764; EC=3.3.2.1;
CC   -!- COFACTOR:
CC       Name=pantetheine 4'-phosphate; Xref=ChEBI:CHEBI:47942;
CC         Evidence={ECO:0000250};
CC       Note=Binds 1 phosphopantetheine covalently. {ECO:0000250};
CC   -!- PATHWAY: Siderophore biosynthesis; vulnibactin biosynthesis.
CC   -!- SIMILARITY: Belongs to the isochorismatase family. {ECO:0000305}.
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DR   EMBL; U32676; AAB18150.1; -; Genomic_DNA.
DR   EMBL; AE016796; AAO07761.1; -; Genomic_DNA.
DR   RefSeq; WP_011081755.1; NC_004460.2.
DR   AlphaFoldDB; P74965; -.
DR   SMR; P74965; -.
DR   EnsemblBacteria; AAO07761; AAO07761; VV2_0838.
DR   KEGG; vvu:VV2_0838; -.
DR   HOGENOM; CLU_068979_2_0_6; -.
DR   OMA; RDIKPFF; -.
DR   UniPathway; UPA00021; -.
DR   Proteomes; UP000002275; Chromosome 2.
DR   GO; GO:0008908; F:isochorismatase activity; IEA:UniProtKB-EC.
DR   Gene3D; 1.10.1200.10; -; 1.
DR   Gene3D; 3.40.50.850; -; 1.
DR   InterPro; IPR036736; ACP-like_sf.
DR   InterPro; IPR016291; Isochorismatase.
DR   InterPro; IPR000868; Isochorismatase-like.
DR   InterPro; IPR036380; Isochorismatase-like_sf.
DR   InterPro; IPR009081; PP-bd_ACP.
DR   Pfam; PF00857; Isochorismatase; 1.
DR   Pfam; PF00550; PP-binding; 1.
DR   PIRSF; PIRSF001111; Isochorismatase; 1.
DR   PRINTS; PR01398; ISCHRISMTASE.
DR   SUPFAM; SSF47336; SSF47336; 1.
DR   SUPFAM; SSF52499; SSF52499; 1.
DR   PROSITE; PS50075; CARRIER; 1.
PE   3: Inferred from homology;
KW   Hydrolase; Phosphopantetheine; Phosphoprotein.
FT   CHAIN           1..295
FT                   /note="Probable isochorismatase"
FT                   /id="PRO_0000201828"
FT   DOMAIN          207..286
FT                   /note="Carrier"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT   REGION          1..21
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         247
FT                   /note="O-(pantetheine 4'-phosphoryl)serine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00258"
FT   CONFLICT        115
FT                   /note="G -> D (in Ref. 1; AAB18150)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        189
FT                   /note="E -> Q (in Ref. 1; AAB18150)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   295 AA;  33205 MW;  2062BA63CD8CB0CC CRC64;
     MGIPKIAGYP LPTPAEFPDN RTGWTIDPDQ AVLLIHDMQE YFVNYYQPDS SPVVDIIQHI
     QRLKAAAKKA GIPVIYTAQP ANQHPTDRAL LTDFWGPGLN GDHVPIVEAL SPEEGDIEYV
     KWRYSAFKKT PLLEFMRAQG KSQLIISGIY GHIGILSTTL DAFMLDIQPF VIGDAIADFT
     REDHLRTLEY VASRSGSVKR LDEALDEIRS QKPLTLEQIQ QDVATSLGIQ PDEVDLDEDL
     MFVGLDSMRA MVLVEKWHQQ GENISFGQLM EAASLREWWL VIEQARNEEQ TMAVA