VENT1_XENLA
ID VENT1_XENLA Reviewed; 264 AA.
AC Q91926;
DT 06-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Homeobox protein vent1;
DE Short=Xvent-1;
GN Name=vent1; Synonyms=vent-1 {ECO:0000303|PubMed:8557046};
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1] {ECO:0000305, ECO:0000312|EMBL:CAA63437.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL
RP STAGE, AND INDUCTION.
RC TISSUE=Embryo {ECO:0000269|PubMed:8557046};
RX PubMed=8557046; DOI=10.1002/j.1460-2075.1995.tb00317.x;
RA Gawantka V., Delius H., Hirschfeld K., Blumenstock C., Niehrs C.;
RT "Antagonizing the Spemann organizer: role of the homeobox gene Xvent-1.";
RL EMBO J. 14:6268-6279(1995).
RN [2] {ECO:0000305}
RP FUNCTION.
RX PubMed=9502725; DOI=10.1242/dev.125.8.1447;
RA Onichtchouk D., Glinka A., Niehrs C.;
RT "Requirement for Xvent-1 and Xvent-2 gene function in dorsoventral
RT patterning of Xenopus mesoderm.";
RL Development 125:1447-1456(1998).
RN [3] {ECO:0000305}
RP FUNCTION, DNA-BINDING, AND DOMAIN.
RX PubMed=9545242; DOI=10.1093/emboj/17.8.2298;
RA Friedle H., Rastegar S., Paul H., Kaufmann E., Knoechel W.;
RT "Xvent-1 mediates BMP-4-induced suppression of the dorsal-lip-specific
RT early response gene XFD-1' in Xenopus embryos.";
RL EMBO J. 17:2298-2307(1998).
RN [4] {ECO:0000305}
RP FUNCTION, AND DNA-BINDING.
RX PubMed=12050139; DOI=10.1242/dev.129.12.2917;
RA Polli M., Amaya E.;
RT "A study of mesoderm patterning through the analysis of the regulation of
RT Xmyf-5 expression.";
RL Development 129:2917-2927(2002).
CC -!- FUNCTION: Transcriptional repressor. Cooperates with vent2 in a ventral
CC signaling pathway downstream of bmp4, which antagonizes the Spemann
CC organizer and dorsal mesoderm formation, and leads to ventral mesoderm
CC formation. Acts downstream of bmp4 to repress transcription of foxa4-
CC B/XFD-1'. Binds to DNA with preference for the target sequence 5'-
CC CTATT[T/C]G-3'. Also binds 5'-TGCATTTTG-3' at a lower frequency, and
CC occasionally 5'-TTGATC-3'. Binds to the homeobox 2 (HBX2) repressor
CC element in the promoter of the myf5 gene and represses myf5
CC transcription in the ventral domain. {ECO:0000269|PubMed:12050139,
CC ECO:0000269|PubMed:8557046, ECO:0000269|PubMed:9502725,
CC ECO:0000269|PubMed:9545242}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in the ventral marginal zone of
CC gastrulae. At stage 11.5, also expressed in the ventral region of the
CC animal cap (ectoderm). At the end of gastrulation, predominantly
CC localized to the ventral and lateral regions of the closing slit
CC blastopore. At early tail bud stage, expression is maintained only in
CC the forming proctodeum. {ECO:0000269|PubMed:8557046}.
CC -!- DEVELOPMENTAL STAGE: Expression begins in the late blastula, peaks at
CC state 11 (early gastrula) and decreases thereafter.
CC {ECO:0000269|PubMed:8557046}.
CC -!- INDUCTION: By bmp4. Suppressed by gsc, acting in a negative cross-
CC regulatory loop. {ECO:0000269|PubMed:8557046}.
CC -!- DOMAIN: The N-terminal region is required for repressor function.
CC {ECO:0000269|PubMed:9545242}.
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DR EMBL; X92851; CAA63437.1; -; mRNA.
DR PIR; S66151; S66151.
DR RefSeq; NP_001091390.1; NM_001097921.1.
DR AlphaFoldDB; Q91926; -.
DR SMR; Q91926; -.
DR GeneID; 100037246; -.
DR KEGG; xla:100037246; -.
DR CTD; 100037246; -.
DR Xenbase; XB-GENE-920881; ventx1.2.S.
DR OrthoDB; 1369499at2759; -.
DR Proteomes; UP000186698; Chromosome 7S.
DR Bgee; 100037246; Expressed in gastrula and 2 other tissues.
DR GO; GO:0005634; C:nucleus; IC:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR GO; GO:0030509; P:BMP signaling pathway; IMP:UniProtKB.
DR GO; GO:0048264; P:determination of ventral identity; IMP:UniProtKB.
DR GO; GO:0001707; P:mesoderm formation; IMP:UniProtKB.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IMP:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..264
FT /note="Homeobox protein vent1"
FT /id="PRO_0000279722"
FT DNA_BIND 129..188
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 16..140
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 59..106
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 107..129
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 264 AA; 29978 MW; 356853895AC555B3 CRC64;
MVQQGFSIDL ILARSKEEAT DGKDSMSSRP HIPCAPQPLP PTKYAKEMPR KKDGQDVQEH
TTSFQCSLGE QVINRPSANP SLAAMHRSSG SSDEFSPPGS EDDSTESSGR SSQENDTEQR
EKSPKSDLQR RLRTAFTPQQ ISKLEQAFNK QRYLGAPERK KLATSLQLSE IQVKTWFQNR
RMKLKRQIQD KQHSLVPPPV CYPQTFPYYP GGFPVPLNSG SFYQPRALPF QAPQHSYIPQ
PLHHHVRMSS HQEQYPPLFG AQYM
