ID   VERB_PENPO              Reviewed;         228 AA.
AC   A0A1V6NVX3;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2017, sequence version 1.
DT   03-AUG-2022, entry version 10.
DE   RecName: Full=Methyltransferase verB {ECO:0000303|PubMed:34093475};
DE            EC=2.1.1.- {ECO:0000305|PubMed:34093475};
DE   AltName: Full=Cluster 4 protein B {ECO:0000303|PubMed:34093475};
DE   AltName: Full=Verrucosidin biosynthesis cluster protein B {ECO:0000303|PubMed:34093475};
GN   Name=verB {ECO:0000303|PubMed:34093475};
GN   Synonyms=cl4B {ECO:0000303|PubMed:34093475}; ORFNames=PENPOL_c002G01780;
OS   Penicillium polonicum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=60169;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IBT 4502;
RX   PubMed=28368369; DOI=10.1038/nmicrobiol.2017.44;
RA   Nielsen J.C., Grijseels S., Prigent S., Ji B., Dainat J., Nielsen K.F.,
RA   Frisvad J.C., Workman M., Nielsen J.;
RT   "Global analysis of biosynthetic gene clusters reveals vast potential of
RT   secondary metabolite production in Penicillium species.";
RL   Nat. Microbiol. 2:17044-17044(2017).
RN   [2]
RP   FUNCTION.
RX   PubMed=34093475; DOI=10.3389/fmicb.2021.660871;
RA   Valente S., Piombo E., Schroeckh V., Meloni G.R., Heinekamp T.,
RA   Brakhage A.A., Spadaro D.;
RT   "CRISPR-Cas9-Based Discovery of the Verrucosidin Biosynthesis Gene Cluster
RT   in Penicillium polonicum.";
RL   Front. Microbiol. 12:660871-660871(2021).
CC   -!- FUNCTION: Methyltransferase; part of the gene cluster that mediates the
CC       biosynthesis of the neurotoxin verrucosidin, a methylated alpha-pyrone
CC       polyketide that inhibits oxidative phosphorylation in mitochondria and
CC       thereby causes neurological diseases (PubMed:34093475). The carbon
CC       backbone of verrucosidin is synthesized by the HR-PKS verA, and further
CC       modified by the other verrucodidin cluster enzymes (Probable).
CC       {ECO:0000269|PubMed:34093475, ECO:0000305|PubMed:34093475}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000305|PubMed:34093475}.
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000305|PubMed:34093475}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC       {ECO:0000255}.
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DR   EMBL; MDYM01000002; OQD68888.1; -; Genomic_DNA.
DR   UniPathway; UPA00213; -.
DR   Proteomes; UP000191408; Unassembled WGS sequence.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR016584; MeTrfase_VrtF.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   PIRSF; PIRSF011491; Mtase_YbcY_prd; 1.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   3: Inferred from homology;
KW   Methyltransferase; Reference proteome; Transferase.
FT   CHAIN           1..228
FT                   /note="Methyltransferase verB"
FT                   /id="PRO_0000455372"
SQ   SEQUENCE   228 AA;  25431 MW;  4FF2FB288FE4BEE1 CRC64;
     MHPSARLYNN IALWFYDYLV HSIFMVYGWH CPTKSVGLPF FSSHVGPKHM DVGVGTGYFP
     VAVRKSSRKR NGQLKPQWPQ KLMLVDLNPN CTAMAATRVG APDCTETLTA DVLQPIPAAG
     KHETFDSLSL MNVLHCLPGT SESKAQAFGH LKPFLKEDGV LFGSTILGRG VEHNRFGRLV
     MWLSNSLGIF HNYGDHPDDF VRALQKDFNQ VEHVVVGRVL LFTAKEPN