ID   VERF_PENPO              Reviewed;         730 AA.
AC   A0A1V6NWD3;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2017, sequence version 1.
DT   25-MAY-2022, entry version 22.
DE   RecName: Full=Transcription factor verF {ECO:0000303|PubMed:34093475};
DE   AltName: Full=Cluster 4 protein F {ECO:0000303|PubMed:34093475};
DE   AltName: Full=Verrucosidin biosynthesis cluster protein F {ECO:0000303|PubMed:34093475};
GN   Name=verF {ECO:0000303|PubMed:34093475};
GN   Synonyms=cl4F {ECO:0000303|PubMed:34093475}; ORFNames=PENPOL_c002G07024;
OS   Penicillium polonicum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=60169;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IBT 4502;
RX   PubMed=28368369; DOI=10.1038/nmicrobiol.2017.44;
RA   Nielsen J.C., Grijseels S., Prigent S., Ji B., Dainat J., Nielsen K.F.,
RA   Frisvad J.C., Workman M., Nielsen J.;
RT   "Global analysis of biosynthetic gene clusters reveals vast potential of
RT   secondary metabolite production in Penicillium species.";
RL   Nat. Microbiol. 2:17044-17044(2017).
RN   [2]
RP   FUNCTION.
RX   PubMed=34093475; DOI=10.3389/fmicb.2021.660871;
RA   Valente S., Piombo E., Schroeckh V., Meloni G.R., Heinekamp T.,
RA   Brakhage A.A., Spadaro D.;
RT   "CRISPR-Cas9-Based Discovery of the Verrucosidin Biosynthesis Gene Cluster
RT   in Penicillium polonicum.";
RL   Front. Microbiol. 12:660871-660871(2021).
CC   -!- FUNCTION: Transcription factor; part of the gene cluster that mediates
CC       the biosynthesis of the neurotoxin verrucosidin, a methylated alpha-
CC       pyrone polyketide that inhibits oxidative phosphorylation in
CC       mitochondria and thereby causes neurological diseases.
CC       {ECO:0000269|PubMed:34093475}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00227}.
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DR   EMBL; MDYM01000002; OQD68940.1; -; Genomic_DNA.
DR   Proteomes; UP000191408; Unassembled WGS sequence.
DR   GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR   GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR   GO; GO:0006351; P:transcription, DNA-templated; IEA:InterPro.
DR   CDD; cd00067; GAL4; 1.
DR   Gene3D; 4.10.240.10; -; 1.
DR   InterPro; IPR007219; Transcription_factor_dom_fun.
DR   InterPro; IPR001138; Zn2-C6_fun-type_DNA-bd.
DR   InterPro; IPR036864; Zn2-C6_fun-type_DNA-bd_sf.
DR   InterPro; IPR036236; Znf_C2H2_sf.
DR   InterPro; IPR013087; Znf_C2H2_type.
DR   Pfam; PF04082; Fungal_trans; 1.
DR   Pfam; PF00172; Zn_clus; 1.
DR   SMART; SM00066; GAL4; 1.
DR   SMART; SM00355; ZnF_C2H2; 2.
DR   SUPFAM; SSF57667; SSF57667; 1.
DR   SUPFAM; SSF57701; SSF57701; 1.
DR   PROSITE; PS00028; ZINC_FINGER_C2H2_1; 1.
DR   PROSITE; PS50157; ZINC_FINGER_C2H2_2; 2.
DR   PROSITE; PS50048; ZN2_CY6_FUNGAL_2; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Metal-binding; Nucleus; Reference proteome; Repeat;
KW   Transcription; Transcription regulation; Zinc; Zinc-finger.
FT   CHAIN           1..730
FT                   /note="Transcription factor verF"
FT                   /id="PRO_0000455373"
FT   ZN_FING         19..41
FT                   /note="C2H2-type 1"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   ZN_FING         47..69
FT                   /note="C2H2-type 2; atypical"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00042"
FT   DNA_BIND        83..109
FT                   /note="Zn(2)-C6 fungal-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00227"
FT   REGION          120..151
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        120..139
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   730 AA;  83492 MW;  9EE583A696A41278 CRC64;
     MSTEYPLVGS PETSEYPKYE CSLCLKRYKR REHLFRHIGS HTSQRPYQCN SCDGAFQRAD
     VLKRHLRTCD GGASRASTRR RACDRCVRQK KACSSHQPCH SCAKKGAQCL YSTDTGYSSR
     LSQHSSTNHT PKDQELSTQF TNPPPPPSTS TFNTMMPWGL TMEDLQNFGT SPTNTFFDPA
     SMQYTSPTLP DFSVLPFEGP ATFETPVSSD PPRKSLHFLD KFTSNTGLVS SFDCGTHEQR
     EQIAARLDQQ ILSELQQRIM SMPALGMDIP SPLIMENNSD TSSTSDLPLD WFNDPLSLKT
     HEILLLVEEV VTIKPRNSSV ALDWSSTLRD ACLQFFSPSN IRRFLGFYWA IWHPNVNFVH
     RPTFDMLAAK PTLLAAMALI GACVSPDMPD NEDARTWFNC VEEMVFIDDD FNSDLTYQYS
     GNIAIQRRKI QAVQAAYIVC LYQNWEGADA SKSRIRRYRF ATLVSTARDI GITAARHLNY
     SELGRHEFEW KEYEAREELI RLFTWIFLLD SAFVIFNNLP PRMVIKEIRM HMATPEACFQ
     ATTADECHHQ LHLFLPARTL YWTTSFRGSF ESLCKDDLSV NIRHLLATLG PLNLFALTSA
     IHSQIFQFRS AVGSFQLRAP IQNALSNWRD IWQLFSSTFP QGITPHMTIE DPHIQPEELW
     KRMGFFRYAP EYWLLAHLMA DRLAVLGTSK PENELEPLDE GPLDPILNRY DQTSMRQVND
     LIMGFQTFQI