ID   VERG_PENPO              Reviewed;         433 AA.
AC   A0A1V6NXN7;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2017, sequence version 1.
DT   03-AUG-2022, entry version 9.
DE   RecName: Full=Acyl-CoA N-acyltransferase verG {ECO:0000303|PubMed:34093475};
DE            EC=2.3.1.- {ECO:0000305|PubMed:34093475};
DE   AltName: Full=Cluster 4 protein G {ECO:0000303|PubMed:34093475};
DE   AltName: Full=Verrucosidin biosynthesis cluster protein G {ECO:0000303|PubMed:34093475};
GN   Name=verG {ECO:0000303|PubMed:34093475};
GN   Synonyms=cl4G {ECO:0000303|PubMed:34093475}; ORFNames=PENPOL_c002G05667;
OS   Penicillium polonicum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=60169;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IBT 4502;
RX   PubMed=28368369; DOI=10.1038/nmicrobiol.2017.44;
RA   Nielsen J.C., Grijseels S., Prigent S., Ji B., Dainat J., Nielsen K.F.,
RA   Frisvad J.C., Workman M., Nielsen J.;
RT   "Global analysis of biosynthetic gene clusters reveals vast potential of
RT   secondary metabolite production in Penicillium species.";
RL   Nat. Microbiol. 2:17044-17044(2017).
RN   [2]
RP   FUNCTION, AND PATHWAY.
RX   PubMed=34093475; DOI=10.3389/fmicb.2021.660871;
RA   Valente S., Piombo E., Schroeckh V., Meloni G.R., Heinekamp T.,
RA   Brakhage A.A., Spadaro D.;
RT   "CRISPR-Cas9-Based Discovery of the Verrucosidin Biosynthesis Gene Cluster
RT   in Penicillium polonicum.";
RL   Front. Microbiol. 12:660871-660871(2021).
CC   -!- FUNCTION: Acyl-CoA N-acyltransferase; part of the gene cluster that
CC       mediates the biosynthesis of the neurotoxin verrucosidin, a methylated
CC       alpha-pyrone polyketide that inhibits oxidative phosphorylation in
CC       mitochondria and thereby causes neurological diseases
CC       (PubMed:34093475). The carbon backbone of verrucosidin is synthesized
CC       by the HR-PKS verA, and further modified by the other verrucodidin
CC       cluster enzymes (Probable). {ECO:0000269|PubMed:34093475,
CC       ECO:0000305|PubMed:34093475}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000305|PubMed:34093475}.
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000305|PubMed:34093475}.
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DR   EMBL; MDYM01000002; OQD69357.1; -; Genomic_DNA.
DR   UniPathway; UPA00213; -.
DR   Proteomes; UP000191408; Unassembled WGS sequence.
DR   GO; GO:0016740; F:transferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
DR   InterPro; IPR016181; Acyl_CoA_acyltransferase.
DR   SUPFAM; SSF55729; SSF55729; 1.
PE   4: Predicted;
KW   Reference proteome; Transferase.
FT   CHAIN           1..433
FT                   /note="Acyl-CoA N-acyltransferase verG"
FT                   /id="PRO_0000455367"
SQ   SEQUENCE   433 AA;  47180 MW;  EEFED0983E78984A CRC64;
     MAICIYEHDA AKIIPTLVSE LPYSTTLLRR IQHGIAYPYE TAHILATFPP GLTPEPGQPW
     LAARVDLFAG RTTQMIIYSS LEAEHTSIPP IVTVSDSTEI LNVDSTSNNS KNNDTNDADN
     PDINSITKFI VSTFSASPPV LALARAQLLA LLEYVKTNML PFYLSYLAKS AQATSVAAET
     PPVSTSSVTS NNTSPTSVPL IPAPDPQAFL IGSLHTGLFS LLQQSGSYTL SNPIPNIRVH
     RFDDPPYYKY FFRRSEFSPE AVCGRSADLP FADLSLPSGY RFHDREGREG VLSKHLDLVQ
     SRTHIPRPRA QLSKIPGMAV YLDSTSSDAD EMPIAWGFLG VDGALVTLHV EPEHRGRGLA
     VPLSKAIMRR GMAVDGVFGA GSVNSEDSQT RERVGAWAHT EVAGYNNASR RVMEKIGGQV
     LTTVTWTVIE LLD