ID   VERK_CLORO              Reviewed;         179 AA.
AC   A0A1U9YI11;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2017, sequence version 1.
DT   25-MAY-2022, entry version 12.
DE   RecName: Full=Gamma-glutamyl cyclotransferase verK {ECO:0000250|UniProtKB:E9R9Y3};
DE            Short=GGCT verK {ECO:0000250|UniProtKB:E9R9Y3};
DE            EC=4.3.2.9 {ECO:0000250|UniProtKB:E9R9Y3};
DE   AltName: Full=Verticillin biosynthesis cluster protein K {ECO:0000303|PubMed:28376389};
GN   Name=verK {ECO:0000303|PubMed:28376389};
OS   Clonostachys rogersoniana.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Bionectriaceae; Clonostachys.
OX   NCBI_TaxID=122658;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   PATHWAY.
RC   STRAIN=XZC04-CC-302;
RX   PubMed=28376389; DOI=10.1016/j.fgb.2017.03.007;
RA   Wang Y., Hu P., Pan Y., Zhu Y., Liu X., Che Y., Liu G.;
RT   "Identification and characterization of the verticillin biosynthetic gene
RT   cluster in Clonostachys rogersoniana.";
RL   Fungal Genet. Biol. 103:25-33(2017).
RN   [2]
RP   INDUCTION.
RX   PubMed=29058652; DOI=10.1099/mic.0.000557;
RA   Guo Z., Hao T., Wang Y., Pan Y., Ren F., Liu X., Che Y., Liu G.;
RT   "VerZ, a Zn(II)2Cys6 DNA-binding protein, regulates the biosynthesis of
RT   verticillin in Clonostachys rogersoniana.";
RL   Microbiology 163:1654-1663(2017).
CC   -!- FUNCTION: Gamma-glutamyl cyclotransferase; part of the gene cluster
CC       that mediates the biosynthesis of 11'-deoxyverticillin A, one of the
CC       dimeric epipolythiodioxopiperazines (ETPs) from the verticillin family
CC       that act as mycotoxins (PubMed:28376389). 11'-deoxyverticillin A is
CC       required for normal conidiation (PubMed:28376389). The nonribosomal
CC       peptide synthetase verP is speculated to be responsible for
CC       condensation of amino acids to form the carbon skeleton of verticillin,
CC       whereas the cluster-specific tailoring enzymes are involved in further
CC       modifications leading to the production of 11'-deoxyverticillin A
CC       (Probable). {ECO:0000269|PubMed:28376389, ECO:0000305|PubMed:28376389}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alpha-(gamma-L-glutamyl)-L-amino acid = 5-oxo-L-proline +
CC         an L-alpha-amino acid; Xref=Rhea:RHEA:20505, ChEBI:CHEBI:58402,
CC         ChEBI:CHEBI:59869, ChEBI:CHEBI:71304; EC=4.3.2.9;
CC         Evidence={ECO:0000250|UniProtKB:E9R9Y3};
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000269|PubMed:28376389}.
CC   -!- INDUCTION: Expression is regulated by the cluster-specific regulator
CC       verZ. {ECO:0000269|PubMed:29058652}.
CC   -!- DISRUPTION PHENOTYPE: Dicreases the production of 11'-deoxyverticillin
CC       A. {ECO:0000269|PubMed:28376389}.
CC   -!- SIMILARITY: Belongs to the class-I pyridoxal-phosphate-dependent
CC       aminotransferase family. {ECO:0000305}.
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DR   EMBL; KY359203; AQZ42164.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1U9YI11; -.
DR   SMR; A0A1U9YI11; -.
DR   GO; GO:0003839; F:gamma-glutamylcyclotransferase activity; IEA:UniProtKB-EC.
DR   CDD; cd06661; GGCT_like; 1.
DR   InterPro; IPR017939; G-Glutamylcylcotransferase.
DR   InterPro; IPR013024; GGCT-like.
DR   PANTHER; PTHR12935; PTHR12935; 1.
PE   2: Evidence at transcript level;
KW   Lyase.
FT   CHAIN           1..179
FT                   /note="Gamma-glutamyl cyclotransferase verK"
FT                   /id="PRO_0000450166"
SQ   SEQUENCE   179 AA;  19953 MW;  7889AA475FE5357D CRC64;
     MTNTERSSSW KASSIPDQPM WYFGYGSNMK ASSMADRKVT PLSTKIVTVP THFVTFDIFG
     IPYSEPSYAS LEQFPDGGTG KLDLVHHISR TQVLPACGVA HLLSPNDFHR LLVTEGSGVV
     YNLVEVQAYE MNKDGQPIPA PFTVHTLKAK WPQRPNGTPS ARYMVRFLGL LSSSTYYQY