VERL_HALRU
ID VERL_HALRU Reviewed; 3722 AA.
AC Q8WR62; Q9BKI0; Q9TZY2; Q9TZY3;
DT 27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 02-JUN-2021, entry version 37.
DE RecName: Full=Vitelline envelope sperm lysin receptor {ECO:0000303|PubMed:12034500};
DE Flags: Precursor;
GN Name=VERL {ECO:0000303|PubMed:28622512, ECO:0000312|EMBL:AAL50827.1};
OS Haliotis rufescens (California red abalone).
OC Eukaryota; Metazoa; Spiralia; Lophotrochozoa; Mollusca; Gastropoda;
OC Vetigastropoda; Lepetellida; Haliotoidea; Haliotidae; Haliotis.
OX NCBI_TaxID=6454 {ECO:0000312|EMBL:AAL50827.1};
RN [1] {ECO:0000312|EMBL:AAL50827.1}
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=12034500; DOI=10.1016/s0378-1119(02)00459-6;
RA Galindo B.E., Moy G.W., Swanson W.J., Vacquier V.D.;
RT "Full-length sequence of VERL, the egg vitelline envelope receptor for
RT abalone sperm lysin.";
RL Gene 288:111-117(2002).
RN [2] {ECO:0000312|EMBL:AAC31410.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 2673-2825 AND 2979-3131.
RX PubMed=9685267; DOI=10.1126/science.281.5377.710;
RA Swanson W.J., Vacquier V.D.;
RT "Concerted evolution in an egg receptor for a rapidly evolving abalone
RT sperm protein.";
RL Science 281:710-712(1998).
RN [3] {ECO:0000312|EMBL:AAK20409.1}
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 3243-3483.
RX PubMed=11230538; DOI=10.1093/oxfordjournals.molbev.a003813;
RA Swanson W.J., Aquadro C.F., Vacquier V.D.;
RT "Polymorphism in abalone fertilization proteins is consistent with the
RT neutral evolution of the egg's receptor for lysin (VERL) and positive
RT darwinian selection of sperm lysin.";
RL Mol. Biol. Evol. 18:376-383(2001).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH LYSIN, SUBUNIT,
RP GLYCOSYLATION, AND DEVELOPMENTAL STAGE.
RX PubMed=9192632; DOI=10.1073/pnas.94.13.6724;
RA Swanson W.J., Vacquier V.D.;
RT "The abalone egg vitelline envelope receptor for sperm lysin is a giant
RT multivalent molecule.";
RL Proc. Natl. Acad. Sci. U.S.A. 94:6724-6729(1997).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.64 ANGSTROMS) OF 38-453 IN COMPLEXES WITH LYSIN,
RP FUNCTION, INTERACTION WITH LYSIN, SUBUNIT, DOMAIN, DISULFIDE BOND,
RP GLYCOSYLATION AT ASN-254; ASN-373 AND ASN-417, AND MUTAGENESIS OF PHE-181;
RP LEU-228; GLU-384; ASN-388 AND MET-389.
RX PubMed=28622512; DOI=10.1016/j.cell.2017.05.033;
RA Raj I., Sadat Al Hosseini H., Dioguardi E., Nishimura K., Han L., Villa A.,
RA de Sanctis D., Jovine L.;
RT "Structural basis of egg coat-sperm recognition at fertilization.";
RL Cell 169:1315-1326(2017).
CC -!- FUNCTION: Structural component of the egg vitelline envelope; forms
CC long filaments. Functions as species-specific receptor for the sperm
CC protein lysin; prevents fertilization by sperm from other species. Each
CC VERL chain can bind multiple copies of the sperm protein lysin; this
CC creates a 3 um hole in the egg vitelline envelope through which the
CC sperm passes. {ECO:0000269|PubMed:28622512,
CC ECO:0000269|PubMed:9192632}.
CC -!- SUBUNIT: May form disulfide-linked homodimers (PubMed:9192632,
CC PubMed:28622512). Interacts (via VERL repeats) with sperm lysin
CC (PubMed:9192632, PubMed:28622512). Each VERL chain can bind numerous
CC lysin molecules (PubMed:9192632, PubMed:28622512).
CC {ECO:0000269|PubMed:28622512, ECO:0000269|PubMed:9192632}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305|PubMed:9192632};
CC Single-pass membrane protein {ECO:0000255}. Secreted, extracellular
CC space, extracellular matrix {ECO:0000305|PubMed:9192632}.
CC -!- DEVELOPMENTAL STAGE: Detected on the egg vitelline envelope (at protein
CC level). {ECO:0000269|PubMed:9192632}.
CC -!- DOMAIN: VERL repeat 2 is important for species-specific lysin binding,
CC even if it has lower affinity for lysin than VERL repeat 3. VERL
CC repeats 3 to 22 have high sequence similarity, indicating they all can
CC bind lysin equally well, but they do not play a role in species-
CC specific sperm selection. VERL repeat 1 does not bind lysin.
CC {ECO:0000269|PubMed:28622512}.
CC -!- PTM: N-glycosylated (PubMed:9192632, PubMed:28622512). About half of
CC the glycoprotein mass corresponds to carbohydrate chains
CC (PubMed:9192632). N-glycosylation is not required for lysin binding
CC (PubMed:28622512). {ECO:0000269|PubMed:28622512,
CC ECO:0000269|PubMed:9192632}.
CC -!- PTM: O-glycosylated. O-glycosylation is not required for lysin binding.
CC {ECO:0000269|PubMed:28622512}.
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DR EMBL; AF453553; AAL50827.1; -; mRNA.
DR EMBL; AF053665; AAC31408.1; -; Genomic_DNA.
DR EMBL; AF053667; AAC31410.1; -; Genomic_DNA.
DR EMBL; AF250899; AAK20409.1; -; Genomic_DNA.
DR PDB; 5II4; X-ray; 2.00 A; A=38-175.
DR PDB; 5II5; X-ray; 1.80 A; A=38-151.
DR PDB; 5IIA; X-ray; 1.70 A; B/D/F/H=340-453.
DR PDB; 5IIB; X-ray; 1.64 A; B=340-453.
DR PDB; 5IIC; X-ray; 2.90 A; A/B=340-453.
DR PDB; 5MR2; X-ray; 2.50 A; A/B/C=176-298.
DR PDB; 5MR3; X-ray; 1.80 A; B/D/F/H=176-298.
DR PDBsum; 5II4; -.
DR PDBsum; 5II5; -.
DR PDBsum; 5IIA; -.
DR PDBsum; 5IIB; -.
DR PDBsum; 5IIC; -.
DR PDBsum; 5MR2; -.
DR PDBsum; 5MR3; -.
DR SMR; Q8WR62; -.
DR iPTMnet; Q8WR62; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-KW.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0060388; C:vitelline envelope; IDA:UniProtKB.
DR GO; GO:0035036; P:sperm-egg recognition; IDA:UniProtKB.
DR InterPro; IPR021526; Vitelline_env_lysin-recpt.
DR InterPro; IPR001507; ZP_dom.
DR Pfam; PF11386; VERL; 22.
DR PROSITE; PS51034; ZP_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell membrane; Disulfide bond; Extracellular matrix;
KW Fertilization; Glycoprotein; Membrane; Receptor; Repeat; Secreted; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..3722
FT /note="Vitelline envelope sperm lysin receptor"
FT /id="PRO_5004317630"
FT TOPO_DOM 25..3698
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 3699..3719
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 3720..3722
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT REPEAT 77..155
FT /note="VERL 1"
FT /evidence="ECO:0000305"
FT REPEAT 216..293
FT /note="VERL 2"
FT /evidence="ECO:0000305"
FT REPEAT 379..455
FT /note="VERL 3"
FT /evidence="ECO:0000305"
FT REPEAT 532..608
FT /note="VERL 4"
FT /evidence="ECO:0000305"
FT REPEAT 685..761
FT /note="VERL 5"
FT /evidence="ECO:0000305"
FT REPEAT 838..914
FT /note="VERL 6"
FT /evidence="ECO:0000305"
FT REPEAT 991..1067
FT /note="VERL 7"
FT /evidence="ECO:0000305"
FT REPEAT 1144..1220
FT /note="VERL 8"
FT /evidence="ECO:0000305"
FT REPEAT 1297..1373
FT /note="VERL 9"
FT /evidence="ECO:0000305"
FT REPEAT 1449..1525
FT /note="VERL 10"
FT /evidence="ECO:0000305"
FT REPEAT 1602..1678
FT /note="VERL 11"
FT /evidence="ECO:0000305"
FT REPEAT 1755..1831
FT /note="VERL 12"
FT /evidence="ECO:0000305"
FT REPEAT 1908..1984
FT /note="VERL 13"
FT /evidence="ECO:0000305"
FT REPEAT 2061..2137
FT /note="VERL 14"
FT /evidence="ECO:0000305"
FT REPEAT 2214..2290
FT /note="VERL 15"
FT /evidence="ECO:0000305"
FT REPEAT 2367..2443
FT /note="VERL 16"
FT /evidence="ECO:0000305"
FT REPEAT 2520..2596
FT /note="VERL 17"
FT /evidence="ECO:0000305"
FT REPEAT 2673..2749
FT /note="VERL 18"
FT /evidence="ECO:0000305"
FT REPEAT 2826..2902
FT /note="VERL 19"
FT /evidence="ECO:0000305"
FT REPEAT 2979..3055
FT /note="VERL 20"
FT /evidence="ECO:0000305"
FT REPEAT 3132..3208
FT /note="VERL 21"
FT /evidence="ECO:0000305"
FT REPEAT 3274..3351
FT /note="VERL 22"
FT /evidence="ECO:0000305"
FT DOMAIN 3408..3670
FT /note="ZP"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00375"
FT REGION 146..169
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 279..335
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 443..488
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 603..641
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 756..794
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 909..947
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1062..1100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1215..1253
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1368..1406
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1519..1556
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1672..1711
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1826..1864
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1979..2017
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2132..2170
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2285..2323
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2438..2476
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2590..2629
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2744..2782
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2897..2935
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3050..3088
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3205..3230
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3345..3407
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 292..306
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..335
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 460..488
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 613..641
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 766..794
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 919..947
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1072..1100
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1225..1253
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1378..1406
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1530..1556
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1683..1711
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1836..1864
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1989..2017
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2142..2170
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2295..2323
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2448..2476
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2601..2629
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2754..2782
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2907..2935
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3060..3088
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3213..3230
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3355..3407
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 115
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 122
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 142
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 171
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 254
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:28622512"
FT CARBOHYD 334
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 373
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:28622512"
FT CARBOHYD 417
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:28622512"
FT CARBOHYD 438
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 487
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 501
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 526
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 570
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 591
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 640
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 654
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 679
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 723
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 744
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 793
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 807
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 832
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 876
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 897
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 946
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 960
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 985
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1029
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1050
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1099
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1113
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1138
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1182
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1203
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1252
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1266
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1291
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1335
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1356
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1405
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1419
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1443
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1487
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1557
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1571
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1596
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1640
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1661
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1710
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1724
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1749
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1793
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1814
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1863
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1877
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1902
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1946
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 1967
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2016
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2030
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2055
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2099
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2120
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2169
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2208
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2252
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2273
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2322
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2336
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2361
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2405
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2426
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2475
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2489
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2514
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2558
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2579
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2628
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2642
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2667
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2711
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2732
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2781
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2795
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2820
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2864
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2885
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2934
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2948
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 2973
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3017
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3038
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3087
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3101
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3126
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3170
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3191
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3229
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3243
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3268
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3312
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3333
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3388
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3401
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3449
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3456
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3559
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT CARBOHYD 3650
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT DISULFID 43..141
FT /evidence="ECO:0000269|PubMed:28622512,
FT ECO:0007744|PDB:5II4, ECO:0007744|PDB:5II5"
FT DISULFID 72..104
FT /evidence="ECO:0000269|PubMed:28622512,
FT ECO:0007744|PDB:5II4, ECO:0007744|PDB:5II5"
FT DISULFID 182..280
FT /evidence="ECO:0000269|PubMed:28622512,
FT ECO:0007744|PDB:5MR2, ECO:0007744|PDB:5MR3"
FT DISULFID 201
FT /note="Interchain (with C-294)"
FT /evidence="ECO:0000305|PubMed:28622512,
FT ECO:0007744|PDB:5MR3"
FT DISULFID 211..243
FT /evidence="ECO:0000269|PubMed:28622512,
FT ECO:0007744|PDB:5MR2, ECO:0007744|PDB:5MR3"
FT DISULFID 294
FT /note="Interchain (with C-201)"
FT /evidence="ECO:0000305|PubMed:28622512,
FT ECO:0007744|PDB:5MR3"
FT DISULFID 345..443
FT /evidence="ECO:0000269|PubMed:28622512,
FT ECO:0007744|PDB:5IIA, ECO:0007744|PDB:5IIB,
FT ECO:0007744|PDB:5IIC"
FT DISULFID 374..406
FT /evidence="ECO:0000269|PubMed:28622512,
FT ECO:0007744|PDB:5IIA, ECO:0007744|PDB:5IIB,
FT ECO:0007744|PDB:5IIC"
FT MUTAGEN 181
FT /note="F->Y: Does not abolish species-specific lysin
FT binding; when associated with P-228."
FT /evidence="ECO:0000269|PubMed:28622512"
FT MUTAGEN 228
FT /note="L->P: Does not abolish species-specific lysin
FT binding; when associated with Y-181."
FT /evidence="ECO:0000269|PubMed:28622512"
FT MUTAGEN 384
FT /note="E->G: No effect on lysin binding."
FT /evidence="ECO:0000269|PubMed:28622512"
FT MUTAGEN 388
FT /note="N->A: Reduces lysin binding."
FT /evidence="ECO:0000269|PubMed:28622512"
FT MUTAGEN 389
FT /note="M->K: Reduces lysin binding. Abolishes lysin
FT binding; when associated with A-388."
FT /evidence="ECO:0000269|PubMed:28622512"
FT CONFLICT 2760
FT /note="E -> Q (in Ref. 2; AAC31408)"
FT /evidence="ECO:0000305"
FT CONFLICT 3471
FT /note="F -> V (in Ref. 3; AAK20409)"
FT /evidence="ECO:0000305"
FT STRAND 39..42
FT /evidence="ECO:0007829|PDB:5II5"
FT STRAND 51..55
FT /evidence="ECO:0007829|PDB:5II5"
FT STRAND 61..63
FT /evidence="ECO:0007829|PDB:5II5"
FT STRAND 65..72
FT /evidence="ECO:0007829|PDB:5II5"
FT STRAND 75..78
FT /evidence="ECO:0007829|PDB:5II5"
FT STRAND 86..91
FT /evidence="ECO:0007829|PDB:5II5"
FT STRAND 96..98
FT /evidence="ECO:0007829|PDB:5II5"
FT STRAND 107..111
FT /evidence="ECO:0007829|PDB:5II5"
FT STRAND 118..129
FT /evidence="ECO:0007829|PDB:5II5"
FT STRAND 134..144
FT /evidence="ECO:0007829|PDB:5II5"
FT STRAND 176..181
FT /evidence="ECO:0007829|PDB:5MR3"
FT STRAND 190..196
FT /evidence="ECO:0007829|PDB:5MR3"
FT STRAND 206..211
FT /evidence="ECO:0007829|PDB:5MR3"
FT STRAND 214..218
FT /evidence="ECO:0007829|PDB:5MR3"
FT STRAND 222..230
FT /evidence="ECO:0007829|PDB:5MR3"
FT STRAND 246..251
FT /evidence="ECO:0007829|PDB:5MR3"
FT STRAND 254..267
FT /evidence="ECO:0007829|PDB:5MR3"
FT STRAND 269..271
FT /evidence="ECO:0007829|PDB:5MR3"
FT STRAND 274..283
FT /evidence="ECO:0007829|PDB:5MR3"
FT STRAND 340..344
FT /evidence="ECO:0007829|PDB:5IIB"
FT STRAND 347..351
FT /evidence="ECO:0007829|PDB:5IIC"
FT STRAND 353..359
FT /evidence="ECO:0007829|PDB:5IIB"
FT STRAND 367..374
FT /evidence="ECO:0007829|PDB:5IIB"
FT STRAND 377..381
FT /evidence="ECO:0007829|PDB:5IIB"
FT STRAND 385..395
FT /evidence="ECO:0007829|PDB:5IIB"
FT HELIX 396..398
FT /evidence="ECO:0007829|PDB:5IIB"
FT STRAND 409..413
FT /evidence="ECO:0007829|PDB:5IIB"
FT STRAND 418..435
FT /evidence="ECO:0007829|PDB:5IIB"
FT STRAND 437..446
FT /evidence="ECO:0007829|PDB:5IIB"
SQ SEQUENCE 3722 AA; 410581 MW; 10207F8261ABD258 CRC64;
MSGMQWSFGF SCLFFLKTVW ICQAFDADTP DPRVLSLDLT LVCSDDKSKQ ATLISYPVTF
KGHVIKDMQI FCKNGWMQMT RGRGINMIRI HYPQTYTSVV PGACVFRGPY SVPTNDSIEM
YNVSVALLWS DGTPTYESLE CNVTKSQASN APEPKASPTS STPQPEAASH NQSKLIDWDV
FCSQNENIPA KFISRLVAPK CLAVEKMDVD CSNGLVPITH EHGFNMMLIQ YTRNKLLDSP
GMCVFWGPYS VPKNDTVVLY TVTARLKWSE GPPTDLSIQC YMPKSPDAPK PESCLSSPPE
PEASPSSNAP EPETYPTSSA PEKVSSDQPA PSHNQSKLID WDVYCSQDES IPAKFISRLV
TSKDQALEKT EINCSNGLVP ITQEFGINMM LIQYTRNELL DSPGMCVFWG PYSVPKNDTV
VLYTVTARLK WSEGPPTNLS IQCYMPKSPV APKPETGPTS NAPEPETYPT SSAPEKVSSD
QPAPSHNQSK LIDWDVYCSQ NESIPAKFIS RLVTSKDQAL EKTEINCSNG LVPITQEFGI
NMMLIQYTRN ELLDSPGMCV FWGPYSVPKN DTVVLYTVTA RLKWSEGPPT NLSIECYMPK
SPVAPKPETG PTSNAPEPET YPTSSAPEKV SSDQPAPSHN QSKLIDWDVY CSQNESIPAK
FISRLVTSKD QALEKTEINC SNGLVPITQE FGINMMLIQY TRNELLDSPG MCVFWGPYSV
PKNDTVVLYT VTARLKWSEG PPTNLSIECY MPKSPVAPKP ETGPSSNAPE PETYPTSSAP
EKVSSDQPAP SHNQSKLIDW DVYCSQNESI PAKFISRLVT SKDQALEKTE INCSNGLVPI
TQEFGINMML IQYTRNELLD SPGMCVFWGP YSVPKNDTVV LYTVTARLKW SEGPPTNLSI
ECYMPKSPVA PKPETGPTSN APEPETYPTS SAPEKVSSDQ PAPSHNQSKL IDWDVYCSQN
ESIPAKFISR LVTSKDQALE KTEINCSNGL VPITHEFGIN MMLIQYTRNE LLDSPGMCVF
WGPYSVPKND TVVLYTVTAR LKWSEGPPTN LSIECYMPKS PVAPKPETGP TSNAPEPETY
PTSSAPEKVS SDQPAPSHNQ SKLIDWDVYC SQNESIPAKF ISRLVTSKDQ ALEKTEINCS
NGLVPITQEF GINMMLIQYT RNELLDSPGM CVFWGPYSVP KNDTVVLYTV TARLKWSEGP
PTNLSIECYM PKSPVAPKPE TGPTSNAPEP ETYPTSSAPE KVSSDQPAPS HNQSKLIDWD
VYCSQNESIP AKFISRLVTS KDQALEKTEI NCSNGLVPIT HKFGINMMLI QYTRNELLDS
PGMCVFWGPY SVPKNDTVVL YTVTARLKWS EGPPTNLSIE CYMPKSPVAH KPETGPTSNA
PEPETYPTSS APEKVSSDQP APSHNQSKLI DWDVYCSQNE SIPAKFISLV TSKDQALEKT
EINCSNGLVP ITHEFGINMM LIQYTRNELL DSPGMCVFWG PYSVPKNDTV VLYTVTARLK
WSEGPPTNLP IECYMPKSPV APKPETGPSS NAPEPETYPT SSAPEKVYSD QPAPSHNQSK
LIDWDVYCSQ NESIPAKFIS RLVTSKDQAL EKTEINCSNG LVPITHEFGI NMMLIQYTRN
ELLDSPGMCV FWGPYSVPKN DTVVLYTVTA RLKWSEGPPT NLSIECYMPK SPVAPKPETG
PTSNAPEPQT YPTSSAPEKV SSDQPAPSHN QSKLIDWDVY CSQNESIPAK FISRLVTSKD
QALEKTEINC SNGLVPITQE FGINMMLIQY TRNELLDSPG MCVFWGPYSV PKNDTVVLYT
VTARLKWSEG PPTNLSIECY MPKSPVAPKP ETGPTSNAPE PETYPTSSAP EKVSSDQPAP
SHNQSKLIDW DVYCSQNESI PAKFISRLVT SKDQALEKTE INCSNGLVPI THEFGINMML
IQYTRNELLD SPGMCVFWGP YSVPKNDTVV LYTVTARLKW SEGPPTNLSI ECYMPKSPVA
PKPETGPTSN APEPETYPTS SAPEKVSSDQ PAPSHNQSKL IDWDVYCSQN ESFPAKFISR
LVTSKDQALE KTEINCSNGL VPITQEFGIN MMLIQYTRNE LLDSPGMCVF WGPYSVPKND
TVVLYTVTAR LKWSEGPPTN LSIECYMPKS PVAPKPETGP TSNAPEPETY PTSSAPEKVS
SDQPAPSHNQ SKLIDWDVYC SQNESIPAKF ISRLVTSKDQ ALEKTEINCS NGLVPITQEF
GINMMLIQYT RNELLDSPGM CVFWGPYSVP KNDTVVLYTV TARLKWSEGP PTNLSIECYM
PKSPVAPKPE TGPTSNAPEP ETYPTSSAPE KVSSDQPAPS HNQSKLIDWD VYCSQNESIP
AKFISRLVTS KDQALEKTEI NCSNGLVPIT QEFGINMMLI QYTRNELLDS PGMCVFWGPY
SVPKNDTVVL YTVTARLKWS EGPPTNLSIE CYMPKSPVAP KPETGPTSNA PEPETYPTSS
APEKVSSDQP APSHNQSKLI DWDVYCSQNE SFPAKFISRL VTSKDQALEK TEINCSNGLV
PITQEFGINM MLIQYTRNEL LDSPGMCVFW GPYSVPKNDT VVLYTVTARL KWSEGPPTNL
SIECYMPKSP VAPKPETGPT SNAPEPQTYP TSSAPEKVSS DQPAPSHNQS KLIDWDVYCS
QNESIPAKFI SRLVTSKDQA LEKTEINCSN GLVPITQEFG INMMLIQYTR NELLDSPGMC
VFWGPYSVPK NDTVVLYTVT ARLKWSEGPP TNLSIECYMP KSPVAPKPET GPTSNAPEPE
TYPTSSAPEK VSSDQPAPSH NQSKLIDWDV YCSQNESFPA KFISRLVTSK DQALEKTEIN
CSNGLVPITH EFGINMMLIQ YTRNELLDSP GMCVFWGPYS VPKNDTVVLY TVTARLKWSE
GPPTNLSIEC YMPKSPVAPK PETGPTSNAP EPQTYPTSSA PEKVSSDQPA PSHNQSKLID
WDVYCSQNES FPAKFISRLV TSKDQALEKT EINCSNGLVP ITQEFGINMM LIQYTRNELL
DSPGMCVFWG PYSVPKNDTV VLYTVTARLK WSEGPPTNLS IECYMPKSPV APKPETGPTS
NAPEPETYPT SSAPEKVSSD QPAPSHNQSK LIDWDVYCSQ NESIPAKFIS RLVTSKDQAL
EKTEINCSNG LVPITQEFGI NMMLIQYTRN ELLDSPGMCV FWGPYSVPKN DTVVLYTVTA
RLKWSEGPPT NLSIECYMPK SPVAPKPETY PTSSAPEKVS SDQPAPSHNQ SKLIDWDVYC
SQNESIPAKF ISRLVTSKDQ ALEKTEINCS NGLVPITHEF GINMMLIQYT RNELLDSPGM
CVFWGPYSVP KNDTVVLYTV TARLKWSEGP PTNLSIECYM PKSPVAPKPE ASPTSNAPEP
QTYPTSSAPG TSPEGSATAA PGTSPEGNTT AARNAYPRKS NQTTSTEDVL DDTSNYIIKV
IPHCRTRGDV ALIEIITDVD LSAVAVCSNG SRHHFNSTDF VHFYLPVSYN FTPSVCAFTR
SKANLFKLHI GVSWKDRLHD VTTQKKDFLI TCTFDPHKSH RGPTSASSEP LIAAKEIQSH
QGPQSDAEEV FLKLVDIRNE TLAAAVPLSK KVRLVGEVHG SSLESGLKPV ACDAVGVQQG
QRYTILRDGC GDGIVFAKDI GFITEGNKAF SPVFEVFKLH GNLHLTFMCN FTLCSHSCDG
SSCSNQRRTR RSMAWQDIPH VADFDSSATP STDMATVQVA LLVAVALLIT QLAGLAIYVN
IN
