ID   VERN_CLORO              Reviewed;         287 AA.
AC   A0A1U9YI04;
DT   17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2017, sequence version 1.
DT   03-AUG-2022, entry version 10.
DE   RecName: Full=N-methyltransferase verN {ECO:0000250|UniProtKB:Q4WMJ1};
DE            EC=2.1.1.- {ECO:0000250|UniProtKB:Q4WMJ1};
DE   AltName: Full=Verticillin biosynthesis cluster protein N {ECO:0000303|PubMed:28376389};
GN   Name=vern {ECO:0000303|PubMed:28376389};
OS   Clonostachys rogersoniana.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Hypocreomycetidae; Hypocreales; Bionectriaceae; Clonostachys.
OX   NCBI_TaxID=122658;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, DISRUPTION PHENOTYPE, AND
RP   PATHWAY.
RC   STRAIN=XZC04-CC-302;
RX   PubMed=28376389; DOI=10.1016/j.fgb.2017.03.007;
RA   Wang Y., Hu P., Pan Y., Zhu Y., Liu X., Che Y., Liu G.;
RT   "Identification and characterization of the verticillin biosynthetic gene
RT   cluster in Clonostachys rogersoniana.";
RL   Fungal Genet. Biol. 103:25-33(2017).
RN   [2]
RP   INDUCTION.
RX   PubMed=29058652; DOI=10.1099/mic.0.000557;
RA   Guo Z., Hao T., Wang Y., Pan Y., Ren F., Liu X., Che Y., Liu G.;
RT   "VerZ, a Zn(II)2Cys6 DNA-binding protein, regulates the biosynthesis of
RT   verticillin in Clonostachys rogersoniana.";
RL   Microbiology 163:1654-1663(2017).
CC   -!- FUNCTION: N-methyltransferase; part of the gene cluster that mediates
CC       the biosynthesis of 11'-deoxyverticillin A, one of the dimeric
CC       epipolythiodioxopiperazines (ETPs) from the verticillin family that act
CC       as mycotoxins (PubMed:28376389). 11'-deoxyverticillin A is required for
CC       normal conidiation (PubMed:28376389). The nonribosomal peptide
CC       synthetase verP is speculated to be responsible for condensation of
CC       amino acids to form the carbon skeleton of verticillin, whereas the
CC       cluster-specific tailoring enzymes are involved in further
CC       modifications leading to the production of 11'-deoxyverticillin A
CC       (Probable). {ECO:0000269|PubMed:28376389, ECO:0000305|PubMed:28376389}.
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000269|PubMed:28376389}.
CC   -!- INDUCTION: Expression is regulated by the cluster-specific regulator
CC       verZ. {ECO:0000269|PubMed:29058652}.
CC   -!- DISRUPTION PHENOTYPE: Completely abolishes the verticillin production.
CC       {ECO:0000269|PubMed:28376389}.
CC   -!- SIMILARITY: Belongs to the methyltransferase superfamily. LaeA
CC       methyltransferase family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; KY359203; AQZ42160.1; -; Genomic_DNA.
DR   AlphaFoldDB; A0A1U9YI04; -.
DR   SMR; A0A1U9YI04; -.
DR   GO; GO:0008168; F:methyltransferase activity; IEA:UniProtKB-KW.
DR   GO; GO:0032259; P:methylation; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.50.150; -; 1.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   SUPFAM; SSF53335; SSF53335; 1.
PE   2: Evidence at transcript level;
KW   Methyltransferase; S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..287
FT                   /note="N-methyltransferase verN"
FT                   /id="PRO_0000450168"
SQ   SEQUENCE   287 AA;  32745 MW;  8222F548F3F05651 CRC64;
     MTEHTVSHAN GSDLENAKKT YMLANNQKEI ERMKNQHEWI KGSFGGLVKA PIDFDKKNQS
     ILDSATADGT WLMDVRSLFP PETELIGFDI APELYPPEGT RPRNVELVTA DLLQGLPAQW
     IGRFDLVHQR FVFPNFETEV IREVLGRLMQ CVKPGGWIQL VEPCAGENVS GPEPKWFLLL
     HKLANQFMRS AVPRDAILAI LHEEGFVNIN IESLDIVIGK HQRNKEMDAR GRRSMRDSVS
     NMYPMITAEQ LGMPKEEARA VLDKFEADMQ KYRTAVRHVI IWAQRPE