ID   VERU1_PENPO             Reviewed;         367 AA.
AC   A0A1V6NYL5;
DT   25-MAY-2022, integrated into UniProtKB/Swiss-Prot.
DT   07-JUN-2017, sequence version 1.
DT   03-AUG-2022, entry version 9.
DE   RecName: Full=Terpene cyclase verU1 {ECO:0000250|UniProtKB:A0A455R4Z0};
DE            EC=5.4.99.- {ECO:0000250|UniProtKB:A0A455R4Z0};
DE   AltName: Full=Cluster 4 protein U1 {ECO:0000303|PubMed:34093475};
DE   AltName: Full=Verrucosidin biosynthesis cluster protein U1 {ECO:0000303|PubMed:34093475};
GN   Name=verU1 {ECO:0000303|PubMed:34093475};
GN   Synonyms=cl4U1 {ECO:0000303|PubMed:34093475}; ORFNames=PENPOL_c002G01858;
OS   Penicillium polonicum.
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes;
OC   Eurotiomycetidae; Eurotiales; Aspergillaceae; Penicillium.
OX   NCBI_TaxID=60169;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IBT 4502;
RX   PubMed=28368369; DOI=10.1038/nmicrobiol.2017.44;
RA   Nielsen J.C., Grijseels S., Prigent S., Ji B., Dainat J., Nielsen K.F.,
RA   Frisvad J.C., Workman M., Nielsen J.;
RT   "Global analysis of biosynthetic gene clusters reveals vast potential of
RT   secondary metabolite production in Penicillium species.";
RL   Nat. Microbiol. 2:17044-17044(2017).
RN   [2]
RP   FUNCTION.
RX   PubMed=34093475; DOI=10.3389/fmicb.2021.660871;
RA   Valente S., Piombo E., Schroeckh V., Meloni G.R., Heinekamp T.,
RA   Brakhage A.A., Spadaro D.;
RT   "CRISPR-Cas9-Based Discovery of the Verrucosidin Biosynthesis Gene Cluster
RT   in Penicillium polonicum.";
RL   Front. Microbiol. 12:660871-660871(2021).
CC   -!- FUNCTION: Terpene cyclase; part of the gene cluster that mediates the
CC       biosynthesis of the neurotoxin verrucosidin, a methylated alpha-pyrone
CC       polyketide that inhibits oxidative phosphorylation in mitochondria and
CC       thereby causes neurological diseases (PubMed:34093475). The carbon
CC       backbone of verrucosidin is synthesized by the HR-PKS verA, and further
CC       modified by the other verrucodidin cluster enzymes (Probable).
CC       {ECO:0000269|PubMed:34093475, ECO:0000305|PubMed:34093475}.
CC   -!- PATHWAY: Secondary metabolite biosynthesis; terpenoid biosynthesis.
CC       {ECO:0000305|PubMed:34093475}.
CC   -!- PATHWAY: Mycotoxin biosynthesis. {ECO:0000305|PubMed:34093475}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the membrane-bound ascI terpene cyclase family.
CC       {ECO:0000305}.
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DR   EMBL; MDYM01000002; OQD69720.1; -; Genomic_DNA.
DR   UniPathway; UPA00213; -.
DR   Proteomes; UP000191408; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016853; F:isomerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016114; P:terpenoid biosynthetic process; IEA:UniProtKB-UniPathway.
PE   3: Inferred from homology;
KW   Glycoprotein; Isomerase; Membrane; Reference proteome; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..367
FT                   /note="Terpene cyclase verU1"
FT                   /id="PRO_0000455374"
FT   TRANSMEM        8..28
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        57..77
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        85..105
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        120..140
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        169..189
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        197..217
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        239..259
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        292..312
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        327..347
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        50
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        352
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   367 AA;  39830 MW;  3056888A745FE6A9 CRC64;
     MSTAVNTIRC SLLLLGLVGI YTVWISSFRN GLFLRNEEFA GKGQLPGTPN ATLRTHFTGI
     DTLDKALGIF VVFYWPVCQG NLRSLSLIAF PAAVGVGEMW ILFALQFSQS NSPTRAMGKM
     AMFGMGLMLV GPGIFLPIYC SLDLSSTHRL DDSPSSAAEG HLCRNLRSCL LGGYYILVIL
     LALPSPAVVS YGSKQGIIAL LQGWPLLVSA MLWLTHLCGK DRTADFQATL STARTSIYIS
     AMACATISHL VPLLISLLAD SSDICPGKVF VPHLAWPSRR VTSVEEGLLR FFQWDYGLGS
     LALLLWAVGL HIQRRQQISQ GINYLRLIPE ALFLSVMMSP CGAAALYLYR HNSTNCASKT
     GDKSGSG