ID   VF199_ASFB7             Reviewed;         199 AA.
AC   Q65198;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1996, sequence version 1.
DT   03-AUG-2022, entry version 60.
DE   RecName: Full=Inner membrane protein E199L {ECO:0000305};
DE            Short=pE199L;
GN   OrderedLocusNames=Ba71V-130; ORFNames=E199L;
OS   African swine fever virus (strain Badajoz 1971 Vero-adapted) (Ba71V)
OS   (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10498;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=11831707; DOI=10.1006/viro.1995.1149;
RA   Yanez R.J., Rodriguez J.M., Nogal M.L., Yuste L., Enriquez C.,
RA   Rodriguez J.F., Vinuela E.;
RT   "Analysis of the complete nucleotide sequence of African swine fever
RT   virus.";
RL   Virology 208:249-278(1995).
RN   [2]
RP   SUBCELLULAR LOCATION.
RX   PubMed=30185597; DOI=10.1128/jvi.01293-18;
RA   Alejo A., Matamoros T., Guerra M., Andres G.;
RT   "A Proteomic Atlas of the African Swine Fever Virus Particle.";
RL   J. Virol. 92:0-0(2018).
RN   [3]
RP   INDUCTION.
RX   PubMed=32075923; DOI=10.1128/jvi.00119-20;
RA   Cackett G., Matelska D., Sykora M., Portugal R., Malecki M., Baehler J.,
RA   Dixon L., Werner F.;
RT   "The African Swine Fever Virus Transcriptome.";
RL   J. Virol. 94:0-0(2020).
RN   [4]
RP   FUNCTION, SUBCELLULAR LOCATION, AND DISULFIDE BOND.
RX   PubMed=32788374; DOI=10.1128/mbio.00789-20;
RA   Matamoros T., Alejo A., Rodriguez J.M., Hernaez B., Guerra M.,
RA   Fraile-Ramos A., Andres G.;
RT   "African Swine Fever Virus Protein pE199L Mediates Virus Entry by Enabling
RT   Membrane Fusion and Core Penetration.";
RL   MBio 11:0-0(2020).
RN   [5]
RP   FUNCTION, AND INTERACTION WITH HOST PYCR2.
RX   PubMed=33830435; DOI=10.1007/s12250-021-00375-x;
RA   Chen S., Zhang X., Nie Y., Li H., Chen W., Lin W., Chen F., Xie Q.;
RT   "African Swine Fever Virus Protein E199L Promotes Cell Autophagy through
RT   the Interaction of PYCR2.";
RL   Virol. Sin. 36:196-206(2021).
CC   -!- FUNCTION: Essential for viral fusion with host endosomal membrane and
CC       core release (PubMed:32788374). Not required for virus morphogenesis
CC       and egress (PubMed:32788374). Induces complete autophagy through the
CC       interaction with and down-regulation of host PYCR2 (PubMed:33830435).
CC       {ECO:0000269|PubMed:32788374, ECO:0000269|PubMed:33830435}.
CC   -!- SUBUNIT: Interacts with host PYCR2; this interaction results in
CC       autophagy activation. {ECO:0000269|PubMed:33830435}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000269|PubMed:32788374,
CC       ECO:0000305|PubMed:30185597}. Host membrane {ECO:0000305}; Single-pass
CC       membrane protein {ECO:0000269|PubMed:32788374}. Note=Found in the
CC       perinuclear cytoplasmic viral factories during assembly
CC       (PubMed:32788374). Part of the virion inner membrane (PubMed:32788374).
CC       {ECO:0000269|PubMed:32788374}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:32075923}.
CC   -!- PTM: Contains intramolecular disulfide bonds.
CC       {ECO:0000269|PubMed:32788374}.
CC   -!- SIMILARITY: Belongs to the asfivirus E199L family. {ECO:0000305}.
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DR   EMBL; U18466; AAA65358.1; -; Genomic_DNA.
DR   RefSeq; NP_042822.1; NC_001659.2.
DR   GeneID; 22220358; -.
DR   KEGG; vg:22220358; -.
DR   Proteomes; UP000000624; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0039520; P:induction by virus of host autophagy; IEA:UniProtKB-KW.
PE   1: Evidence at protein level;
KW   Activation of host autophagy by virus; Disulfide bond; Host membrane;
KW   Host-virus interaction; Late protein; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Virion.
FT   CHAIN           1..199
FT                   /note="Inner membrane protein E199L"
FT                   /id="PRO_0000373581"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   199 AA;  22042 MW;  8343C9CD645B357B CRC64;
     MSCMPVSTKC NDIWVDFSCT GPSISELQKK EPKAWAAILR SHTNQQTAED DNIIGSICDK
     QGLCSKDEYA YSQYCACVNS GTLWAECAFA PCNGNKNAYK TTEHRNILTN KQCPSGLTIC
     QNIAEYGGSG NISDLYQNFN CNSVINTFLI NVMNHPFLTL ILIILILIII YRLMPSSGGK
     HNDDKLPPPS LIFSNLNNF