ID   VF199_ASFK5             Reviewed;         199 AA.
AC   P0CA94;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 1.
DT   03-AUG-2022, entry version 22.
DE   RecName: Full=Inner membrane protein E199L;
DE            Short=pE199L;
GN   OrderedLocusNames=Ken-142;
OS   African swine fever virus (isolate Pig/Kenya/KEN-50/1950) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=561445;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Essential for viral fusion with host endosomal membrane and
CC       core release (By similarity). Not required for virus morphogenesis and
CC       egress (By similarity). Induces complete autophagy through the
CC       interaction with and down-regulation of host PYCR2 (By similarity).
CC       {ECO:0000250|UniProtKB:Q65198}.
CC   -!- SUBUNIT: Interacts with host PYCR2; this interaction results in
CC       autophagy activation. {ECO:0000250|UniProtKB:Q65198}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250|UniProtKB:Q65198}.
CC       Host membrane {ECO:0000305}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q65198}. Note=Found in the perinuclear
CC       cytoplasmic viral factories during assembly (By similarity). Part of
CC       the virion inner membrane (By similarity).
CC       {ECO:0000250|UniProtKB:Q65198}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the asfivirus E199L family. {ECO:0000305}.
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DR   EMBL; AY261360; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   Proteomes; UP000000861; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
PE   3: Inferred from homology;
KW   Glycoprotein; Host membrane; Late protein; Membrane; Transmembrane;
KW   Transmembrane helix; Virion.
FT   CHAIN           1..199
FT                   /note="Inner membrane protein E199L"
FT                   /id="PRO_0000373582"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        131
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   199 AA;  21945 MW;  4145B38E9BCD42A6 CRC64;
     MSCTPVSTKC NDIWIDFSCT GPSVSELQKK EPNAWAAILR SQKNQQTAED DTIIGSICDK
     QGLCSKDEYA YSQYCACVNS GTLWAECAFA PCNGNKNAYK TTEQRNILTN KQCPSGLTIC
     QNIAEYGGTG NISDLYQNFN CNSVINTFLI NVMNHPFLTL ILIILILVII YRLMSSSAAK
     QNGDKLPPPS LIFSNLNNF