ID   VF199_ASFM2             Reviewed;         199 AA.
AC   Q65242;
DT   05-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-MAY-2009, sequence version 2.
DT   03-AUG-2022, entry version 56.
DE   RecName: Full=Inner membrane protein E199L {ECO:0000305};
DE            Short=pE199L;
GN   OrderedLocusNames=Mal-138; ORFNames=j18L;
OS   African swine fever virus (isolate Tick/Malawi/Lil 20-1/1983) (ASFV).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Asfuvirales; Asfarviridae; Asfivirus.
OX   NCBI_TaxID=10500;
OH   NCBI_TaxID=6937; Ornithodoros (relapsing fever ticks).
OH   NCBI_TaxID=85517; Phacochoerus aethiopicus (Warthog).
OH   NCBI_TaxID=41426; Phacochoerus africanus (Warthog).
OH   NCBI_TaxID=273792; Potamochoerus larvatus (Bushpig).
OH   NCBI_TaxID=9823; Sus scrofa (Pig).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=8021596; DOI=10.1099/0022-1317-75-7-1655;
RA   Dixon L.K., Twigg S.R.F., Baylis S.A., Vydelingum S., Bristow C.,
RA   Hammond J.M., Smith G.L.;
RT   "Nucleotide sequence of a 55 kbp region from the right end of the genome of
RT   a pathogenic African swine fever virus isolate (Malawi LIL20/1).";
RL   J. Gen. Virol. 75:1655-1684(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Kutish G.F., Rock D.L.;
RT   "African swine fever virus genomes.";
RL   Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   SUBCELLULAR LOCATION.
RX   PubMed=8609490; DOI=10.1099/0022-1317-77-5-941;
RA   Sun H., Jenson J., Dixon L.K., Parkhouse R.M.E.;
RT   "Characterization of the African swine fever virion protein j18L.";
RL   J. Gen. Virol. 77:941-946(1996).
CC   -!- FUNCTION: Essential for viral fusion with host endosomal membrane and
CC       core release (By similarity). Not required for virus morphogenesis and
CC       egress (By similarity). Induces complete autophagy through the
CC       interaction with and down-regulation of host PYCR2 (By similarity).
CC       {ECO:0000250|UniProtKB:Q65198}.
CC   -!- SUBUNIT: Interacts with host PYCR2; this interaction results in
CC       autophagy activation. {ECO:0000250|UniProtKB:Q65198}.
CC   -!- SUBCELLULAR LOCATION: Virion membrane {ECO:0000250|UniProtKB:Q65198}.
CC       Host membrane {ECO:0000305}; Single-pass membrane protein
CC       {ECO:0000250|UniProtKB:Q65198}. Note=Found in the perinuclear
CC       cytoplasmic viral factories during assembly (By similarity). Part of
CC       the virion inner membrane (By similarity).
CC       {ECO:0000250|UniProtKB:Q65198}.
CC   -!- INDUCTION: Expressed in the late phase of the viral replicative cycle.
CC       {ECO:0000305}.
CC   -!- PTM: Contains intramolecular disulfide bonds.
CC       {ECO:0000250|UniProtKB:Q65198}.
CC   -!- SIMILARITY: Belongs to the asfivirus E199L family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CAA50838.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; X71982; CAA50838.1; ALT_FRAME; Genomic_DNA.
DR   EMBL; AY261361; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   TCDB; 1.G.23.1.1; african swine fever virus (asfv) fusion protein pe199l (asfv-fp) family.
DR   PRIDE; Q65242; -.
DR   Proteomes; UP000000860; Genome.
DR   GO; GO:0033644; C:host cell membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0055036; C:virion membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0039520; P:induction by virus of host autophagy; IEA:UniProtKB-KW.
PE   3: Inferred from homology;
KW   Activation of host autophagy by virus; Disulfide bond; Glycoprotein;
KW   Host membrane; Host-virus interaction; Late protein; Membrane;
KW   Transmembrane; Transmembrane helix; Virion.
FT   CHAIN           1..199
FT                   /note="Inner membrane protein E199L"
FT                   /id="PRO_0000373583"
FT   TRANSMEM        150..170
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        131
FT                   /note="N-linked (GlcNAc...) asparagine; by host"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        50
FT                   /note="D -> N (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        59
FT                   /note="D -> A (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        73
FT                   /note="Q -> H (in Ref. 1)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        79..82
FT                   /note="NSGT -> KLRL (in Ref. 1)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   199 AA;  22065 MW;  B74691723E43E42D CRC64;
     MSCMPISTKC NDIWVDFSCT GPSLSELQKK EPKAWAAILR SQRSQQTAED DTIIGSICDK
     QGLCSKNEYA YSQYCACVNS GTLWAECAFA PCNGNKNAYK TTEHRNILTN KQCPSGLTIC
     QNIAEYGGTG NISDLYQNFN CNSVINTFLI NVMNHPFLTL ILIILILVII YRLMSSSGGK
     HNEDKLPPPS LIFSNLNNF